Detection, in silico analysis and molecular diversity of phytoplasmas from solanaceous crops in Turkey

https://doi.org/10.17221/115/2021-PPSCitation:

Usta M., Güller A., Sipahioğlu H.M. (2022): Detection, in silico analysis and molecular diversity of phytoplasmas from solanaceous crops in Turkey. Plant Protect. Sci., 58:31-39.

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Phytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves and fruit malformation in pepper (Capsicum annuum L.), and aerial tuber formation in potato (S. tuberosum L.) during the survey performed in the late season (August to September) of 2015 and 2016 in Van province (Turkey). A total of 100 samples were tested by nested-PCR using universal primer pairs to assess the sanitary status of the solanaceous crops and to characterise the phytoplasma isolates. Among them, seven samples resulted in a 1.25 kb DNA fragment, and five (two eggplants, two peppers, and one potato) were molecularly characterised (Accession No.: KY579357, KT595210, MF564267, MF564266, and MH683601). BLAST and the virtual restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes revealed the presence of two distinct phytoplasma infections in solanaceous crops: ‘Candidatus Phytoplasma trifolii’ a member of the clover proliferation group (16SrVI) and subgroup A and ‘Candidatus P. solani’ a member of the stolbur group (16SrXII) and subgroup A. The virtual RFLP analysis and calculated coefficients of RFLP pattern similarities further revealed a remarkable genetic diversity among the ‘Candidatus P. solani’ isolates infecting pepper (similarity coefficient of 0.90) and eggplant (similarity coefficients of 0.98 and 1.00) at the same geographical area. This is the first report of the natural occurrence of ‘Candidadtus P. trifolii’ in potato from the Eastern Anatolia region, Turkey.

References:
Acosta-Pérez K.I., Piñol-Pérez B.E., Zamora-Gutierrez L., Quiñones-Pantoja M.L., Miranda-Cabrera I., Leyva-López N.E., Arocha-Rosete Y. (2017): A phytoplasma representative of a new subgroup 16SrI-Z associated with Bunchy Top Symptoms (BTS) on papaya in Cuba. La Revista de Protección Vegetal, 32: 52–59.
 
Alma A., Tedeschi R., Lessio F., Picciau L., Gonella E., Ferracini C. (2015): Insect vectors of plant pathogenic Mollicutes in the Euro-Mediterranean region. Phytopathogenic Mollicutes, 5: 53–73.  https://doi.org/10.5958/2249-4677.2015.00063.8
 
Alp Ş., Usta M., Sipahioğlu H.M., Güller A. (2016): First report of “Candidatus Phytoplasma solani” on a new host marigold (Tagetes erecta L.). Turkish Journal of Agriculture and Forestry, 40: 311–318. https://doi.org/10.3906/tar-1506-58
 
Arocha-Rosete Y., Zunnoon-Khan S., Krukovets I., Crosby W., Scott J., Bertaccini A., Michelutti R. (2011): Identification and molecular characterization of the phytoplasma associated with peach rosette-like disease at the Canadian Clonal Genebank based on the 16S rRNA gene analysis. Canadian Journal of Plant Pathology, 33: 127–134. https://doi.org/10.1080/07060661.2011.558854
 
Bertaccini A., Duduk B. (2010): Phytoplasma and phytoplasma diseases: A review of recent research. Phytopathologia Mediterranea, 48: 355–378.
 
Bertaccini A., Duduk B., Paltrinieri S., Contaldo N. (2014): Phytoplasmas and phytoplasma diseases: A severe treat to agriculture. American Journal of Plant Sciences, 5: 1763–1788.  https://doi.org/10.4236/ajps.2014.512191
 
Çağlar B.K., Elbeaıno T., Küsek M., Pehlivan D., Fidan H., Portakaldalı M. (2010): Stolbur phytoplasma infections in potato and tomato plants from different locations in Turkey. Journal of Turkish Phytopathology, 39: 1–8.
 
Choueiri E., Salar P., Jreijiri F., El Zammar S., Massaad R., Abdul-Nour H., Bové J.M., Danet J.L., Foissac X. (2007): Occurrence and distribution of ‘Candidatus Phytoplasma trifolii’ associated with diseases of solanaceous crops in Lebanon. European Journal of Plant Pathology, 118: 411–416.  https://doi.org/10.1007/s10658-007-9142-8
 
Cvrković T., Jović J., Mitrović M., Krstić O., Toševski I. (2013): Experimental and molecular evidence of Reptalus panzeri as a natural vector of bois noir. Plant Pathology, 63: 42–53. https://doi.org/10.1111/ppa.12080
 
Delić D., Contaldo N., Lolić B., Moravčević D., Bertaccini A. (2016): First report of ‘Candidatus Phytoplasma solani’ in pepper and celery in Bosnia and Herzegovina. The Journal of Turkish Phytopathology, 98: 184.
 
Doi Y., Teratiaka M., Yora K., Asuyama H. (1967): Mycoplasma- or PLT group-like micro-organisms found in the phloem elements of plants infected with mulberry dwarf, potato witches’ broom, aster yellows, or Paulownia witches’ broom. Annals of the Phytopathological Society of Japan, 33: 259–266. Japanese with English abstract. https://doi.org/10.3186/jjphytopath.33.259
 
Duduk B., Bertaccini A. (2011): Phytoplasma classification: Taxonomy based on 16S ribosomal gene. Is it enough? Phytopathogenic Mollicutes, 1: 3–13. https://doi.org/10.5958/j.2249-4669.1.1.001
 
Ember I., Acs Z., Munyaneza J.E., Crosslin J.M., Kolber M. (2011): Survey and molecular detection of phytoplasmas associated with potato in Romania and southern Russia. European Journal of Plant Pathology, 130: 367–377.  https://doi.org/10.1007/s10658-011-9759-5
 
Gundersen D.E., Lee I.M. (1996): Ultrasensitive detection of phytoplasmas by nested-PCR assay using two universal primer pairs. Phytopathologia Mediterranea, 35: 144–151.
 
Holeva M.C., Glynos P.E, Karafla C.D., Koutsioumari E.M. (2014): First report of ‘Candidatus Phytoplasma solani’ associated with potato plants in Greece. Plant Disease, 98: 1739. https://doi.org/10.1094/PDIS-05-14-0488-PDN
 
IRPCM Phytoplasma/Spiroplasma Working Team – Phytoplasma Taxonomy Group (2004): ‘Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonise plant phloem and insects. International Journal of Systematic and Evolutionary Microbiology, 54: 1243–1255. https://doi.org/10.1099/ijs.0.02854-0
 
Johannesen J., Foissac X., Kehrli P., Maixner M. (2012): Impact of vector dispersal and host-plant fidelity on the dissemination of an emerging plant pathogen. PLoS One, 7: e51809. doi: 10.1371/journal.pone.0051809 https://doi.org/10.1371/journal.pone.0051809
 
Kra K.D., Toualy M.N.Y., Kouamé A.E.P., Séka K., Kwadjo K.E., Diallo H.A., Bertaccini A., Arocha-Rosete Y. (2017): New phytoplasma subgroup identified from Arecaceae palm species in Grand-Lahou, Côte d’Ivoire. Canadian Journal of Plant Pathology, 39: 297–306. https://doi.org/10.1080/07060661.2017.1354331
 
Langer M., Darimont H., Maixner M. (2003): Control of phytoplasma vectors in organic viticulture. IOBC/wprs Bulletin, 26: 197–203.
 
Lee I.M., Gundersen D.E., Hammond R.W., Davis R.E. (1994): Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology, 84: 559–566.  https://doi.org/10.1094/Phyto-84-559
 
Lee I.M., Gundersen-Rindal D.E., Davis R.E., Bartoszyk I.M. (1998): Revised classification scheme of phytoplasmas based on RFLP analysis of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic and Evolutionary Microbiology, 48: 1153–1169.
 
Maejima K., Oshima K., Namba S. (2014): Exploring the phytoplasmas, plant pathogenic bacteria. Journal of General Plant Pathology, 80: 210–221.  https://doi.org/10.1007/s10327-014-0512-8
 
Marcone C. (2014): Molecular biology and pathogenicity of phytoplasmas. Annals of Applied Biology, 165: 199–221.  https://doi.org/10.1111/aab.12151
 
Mitrović M., Jakovljević M., Jović J., Krstić O., Kosovac A., Toševski I., Cvrković T. (2015): ‘Candidatus Phytoplasma solani’ genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (Hemiptera, Cixiidae) as natural vectors. European Journal of Plant Pathology, 144: 619–630.  https://doi.org/10.1007/s10658-015-0800-y
 
Mori N., Pavan F., Bondavalli R., Reggiani N., Paltrinieri S., Bertaccini A. (2008): Factors affecting the spread of “Bois Noir” disease in north Italy vineyards. Vitis, 47: 65–72.
 
Naderali N., Nejat N., Vadamalai G., Davis R.E., Wei W., Harrison N.A., Kong L., Kadir J., Tan Y.H., Zhao Y. (2017): ‘Candidatus Phytoplasma wodyetiae’, a new taxon associated with yellow decline disease of foxtail palm (Wodyetia bifurcata) in Malaysia. International Journal of Systematic and Evolutionary Microbiology, 67: 3765–3772.  https://doi.org/10.1099/ijsem.0.002187
 
Nejat N., Vadamalai G. (2013): Diagnostic techniques for detection of phytoplasma diseases: Past and present. Journal of Plant Diseases and Protection, 120: 16–25. https://doi.org/10.1007/BF03356449
 
Oksal H.D., Apak F.K., Oksal E., Tursun N., Sipahioğlu H.M. (2017): Detection and molecular characterization of two ‘Candidatus Phytoplasma trifolii’ isolates infecting peppers at the same ecological niche. International Journal of Agriculture and Biology, 19: 1372–1378.
 
Pracros P., Renaudin J., Eveillard S., Mouras A., Hernould M. (2006): Tomato flower abnormalities induced by stolbur phytoplasma infection are associated with changes of expression of floral development genes. Molecular Plant-Microbe Interactions, 19: 62–68. https://doi.org/10.1094/MPMI-19-0062
 
Přibylová J., Spak J. (2013): Dodder transmission of phytoplasmas. Phytoplasma, 938: 41–46.
 
Rao G.P., Mishra M.K., Mishra A., Rao A., Goel S. (2018): Identification and characterization of ‘Candidatus Phytoplasma trifolii’ (16SrVI-D) inducing shoot proliferation disease of potato in India. Indian Phytopathology, 71: 75–78.  https://doi.org/10.1007/s42360-018-0011-5
 
Sertkaya G., Martini M., Musetti R., Osler R. (2007): Detection and molecular characterization of phytoplasmas infecting sesame and solanaceous crops in Turkey. Bulletin of Insectology, 60: 141–142.
 
Shah V.V., Shah N.D., Patrekar P.V. (2013): Medicinal plants from Solanaceae family. Research Journal of Pharmacy and Technology, 6: 143–151.
 
Sugio A., Kingdom H.N., MacLean A.M., Grieve V.M., Hogenhout S.A. (2011): Phytoplasma protein effector SAP11 enhances insect vector reproduction by manipulating plant development and defense hormone biosynthesis. Proceedings of the National Academy of Sciences, 108: 1254–1263. https://doi.org/10.1073/pnas.1105664108
 
Usta M., Güller A., Sipahioğlu H.M. (2017): Detection and characterization of two phytoplasma lineages on cucumber (Cucumis sativus L.) with same symptomatology based on virtual RFLP and nucleotide sequence analysis of 16S rDNA. Yuzuncu Yil University Journal of Agricultural Sciences, 27: 299–308.
 
Usta M., Güller A., Sipahioğlu H.M. (2018): Molecular analysis of ‘Candidatus Phytoplasma trifolii’ and ‘Candidatus Phytoplasma solani’ associated with phytoplasma diseases of tomato (PDT) in Turkey. International Journal of Agriculture and Biology, 20: 1991–1996.
 
Usta M., Güller A., Sipahioğlu H.M. (2021): First report of ‘Candidatus Phytoplasma trifolii’ associated with leaf reddening and upright growth in pears (Pyrus communis L.). Plant Protection Science, 57: 188–195.  https://doi.org/10.17221/163/2020-PPS
 
Venkataravanappa V., Prasanna H.C., Lakshminarayana C.N., Reddy M.K. (2018): Molecular detection and characterization of phytoplasma in association with begomovirus in eggplant. Acta Virologica, 62: 246–258.  https://doi.org/10.4149/av_2018_218
 
Wei W., Davis R.E., Leep I.M., Zhao Y. (2007): Computer-simulated RFLP analysis of 16S rRNA genes: Identification of ten new phytoplasma groups. International Journal of Systematic and Evolutionary Microbiology, 57: 1855–1867.  https://doi.org/10.1099/ijs.0.65000-0
 
Weintraub P.G., Beanland L. (2006): Insect vectors of phytoplasmas. Annual Review of Entomology, 51: 91–111.  https://doi.org/10.1146/annurev.ento.51.110104.151039
 
Zhao Y., Sun Q., Wei W., Davis R.E., Wu W., Liu Q. (2009): ‘Candidatus Phytoplasma tamaricis’, a novel taxon discovered in witches’-broom-diseased salt cedar (Tamarix chinensis Lour.). International Journal of Systematic and Evolutionary Microbiology, 59: 2496–2504. https://doi.org/10.1099/ijs.0.010413-0
 
Zibadoost S., Rastgou M., Asghari-Tazehkand S. (2016): Detection and molecular identification of ‘Candidatus phytoplasma trifoli’ infecting some cultivated crops and vegetables in West Azarbaijan province. Australasian Plant Disease Notes, 11: 3. doi: 10.1007/s13314-015-0188-8 https://doi.org/10.1007/s13314-015-0188-8
 
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