Virulence of new strain of Heterorhabditis bacteriophora from Croatia against Lasioptera rubi

https://doi.org/10.17221/119/2018-PPSCitation:Majić I., Sarajlić A., Lakatos T., Tóth T., Raspudić E., Puškadija Z., Kanižai Šarić G., Laznik Ž. (2019): Virulence of new strain of Heterorhabditis bacteriophora from Croatia against Lasioptera rubi. Plant Protect. Sci., 55: 134-141.
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Soil samples from 100 cultivated and natural sites were assessed for the presence of entomopathogenic nematodes. Heterorhabditid nematodes were recovered from three soil samples during spring months, with the overall positive sample rate of 3%. The isolates of entomopathogenic nematodes were identified as three different strains conspecific with Heterorhabditis bacteriophora (Heterorhabditidae). They were found from natural sites and vineyard, while no recovery occurred from intensively cultivated agricultural fields. The morphometrical characteristics of infective juveniles and males showed differences between all Croatian strains and from the original description. Heterorhabditis bacteriophora ISO9 was bioassayed on Lasioptera rubi (Cecidomyiidae) (the raspberry gall midge) larvae at different nematode concentrations under laboratory conditions. The significantly highest mortality was observed in treatments with 50 and 200 infective juveniles per insect larvae within 8 days after inoculation. This is the first report of entomopathogenic nematodes of the family Heterorhabditidae from Croatia, and susceptibility of L. rubi larvae to entomopathogenic nematodes. The Croatian strain H. bacteriophora ISO9 was proved to possess strong insecticidal properties against L. rubi larvae.

References:
Burnell Ann, Stock S. Patricia (2000): Heterorhabditis, Steinernema and their bacterial symbionts — lethal pathogens of insects. Nematology, 2, 31-42 https://doi.org/10.1163/156854100508872
 
Corlay Favio, Boivin Guy, Bélair Guy (2007): Efficiency of natural enemies against the swede midge Contarinia nasturtii (Diptera: Cecidomyiidae), a new invasive species in North America. Biological Control, 43, 195-201 https://doi.org/10.1016/j.biocontrol.2007.08.002
 
Heriberto Cruz-Martínez, Jaime Ruiz-Vega, Pastor T Matadamas-Ortíz, Carlos I Cortés-Martínez, Jesusita Rosas-Diaz (2017): Formulation of entomopathogenic nematodes for crop pest control – a review. Plant Protection Science, 53, 15-24 https://doi.org/10.17221/35/2016-PPS
 
Denno R.F., Gruner D.S., Kaplan I. (2008): Potential for entomopathogenic nematodes in biological control: a meta-analytical synthesis and insights from trophic cascade theory. Journal of Nematology, 40: 61–72.
 
Evans B. G., Jordan K. S., Brownbridge M., Hallett R. H. (2015): Effect of Temperature and Host Life Stage on Efficacy of Soil Entomopathogens Against the Swede Midge (Diptera: Cecidomyiidae). Journal of Economic Entomology, 108, 473-483 https://doi.org/10.1093/jee/tov050
 
Glazer I. (2002): Survival biology. In: Gaugler R. (ed.): Entomopathogenic Nematology. Wallingford, CAB International: 169–187.
 
Glazer I., Kozodoi E., Salame L., Nestel D. (1996): Spatial and Temporal Occurrence of Natural Populations ofHeterorhabditisspp. (Nematoda:Rhabditida) in a Semiarid Region. Biological Control, 6, 130-136 https://doi.org/10.1006/bcon.1996.0016
 
Hazir Selçuk, Stock S.Patricia, Kaya Harry K, Koppenhöfer Albrecht M, Keskin Nevin (2001): Developmental Temperature Effects on Five Geographic Isolates of the Entomopathogenic Nematode Steinernema feltiae (Nematoda: Steinernematidae). Journal of Invertebrate Pathology, 77, 243-250 https://doi.org/10.1006/jipa.2001.5029
 
Hazir S., Keskin N., Stock S.P., Kaya H.K., Özcan S. (2003): Diversity and distribution of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Turkey. Biodiversity and Conservation, 12: 375–386.https://doi.org/10.1023/A:1021915903822
 
Hominick W.M., Briscoe B.R., del Pino F.G., Heng Jian, Hunt D.J., Kozodoy E., Mracek Z., Nguyen K.B., Reid A.P., Spiridonov S., Stock P., Sturhan D., Waturu C., Yoshida M. (1997): Biosystematics of entomopathogenic nematodes: current status, protocols and definitions. Journal of Helminthology, 71, 271- https://doi.org/10.1017/S0022149X00016096
 
Hunt D.J., Nguyen K.B. (2016): Advances in Entomopathogenic Nematode Taxonomy and Phylogeny. Series: Nematology Monographs and Perspectives. Vol. 12. Leiden, Brill Academic Pub.
 
Iqbal Mudassir, Ehlers Ralf-Udo, Waeyenberge Lieven (2016): Molecular characterisation of novel isolates of entomopathogenic nematodes. Nematology, 18, 277-291 https://doi.org/10.1163/15685411-00002959
 
Kaya H.K., Stock P. (1997): Techniques in insect nematology. In: Lacey L.A. (ed.): Manual of Techniques in Insect Pathology. London, Academic Press: 281–324.
 
Koppenhöfer A.M. (2007): Nematodes. In: Lacey L.A., Kaya H.K. (eds): Field Manual Techniques in Invertebrate Pathology: Application and Evaluation of Pathogens for Control of Insects and other Invertebrate Pests. Dordrecht, Springer: 249–264.
 
Koppenhöfer Albrecht M., Fuzy Eugene M. (2006): Effect of soil type on infectivity and persistence of the entomopathogenic nematodes Steinernema scarabaei, Steinernema glaseri, Heterorhabditis zealandica, and Heterorhabditis bacteriophora. Journal of Invertebrate Pathology, 92, 11-22 https://doi.org/10.1016/j.jip.2006.02.003
 
Koppenhofer Albrecht M., Kaya Harry K. (1995): Density-Dependent Effects on Steinernema glaseri (Nematoda: Steinernematidae) within an Insect Host. The Journal of Parasitology, 81, 797- https://doi.org/10.2307/3283982
 
Lacey L.A., Georgis R. (2012): Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production. Journal of Nematology, 44: 218–225.
 
Laznik Ź., Tóth T., Lakatos T., Trdan S. (2009): Heterorhabditis bacteriophora (Poinar) – the first member from Heterorhabditidae family in Slovenia. Acta Agriculturae Slovenica, 93: 181–187.
 
Laznik Ž., Tóth T., Lakatos T., Vidrih M., Trdan S. (2010): Control of the Colorado potato beetle (Leptinotarsa decemlineata [Say]) on potato under field conditions: a comparison of the efficacy of foliar application of two strains of Steinernema feltiae (Filipjev) and spraying with thiametoxam. Journal of Plant Diseases and Protection, 117, 129-135 https://doi.org/10.1007/BF03356348
 
Majić I., Sarajlić A., Lakatos T., Tóth T., Raspudić E., Zebec V., Šarić G. Kanižai, Kovačić M., Laznik Ž. (2018): First report of entomopathogenic nematode Steinernema feltiae (Rhabditida: Steinernematidae) from Croatia. Helminthologia, 55, 256-260 https://doi.org/10.2478/helm-2018-0024
 
Mráček Zdeněk, Bečvář Stanislav, Kindlmann Pavel, Jersáková Jana (2005): Habitat preference for entomopathogenic nematodes, their insect hosts and new faunistic records for the Czech Republic. Biological Control, 34, 27-37 https://doi.org/10.1016/j.biocontrol.2005.03.023
 
Mukuka J., Strauch O., Hoppe C., Ehlers R.U. (2010): Improvement of heat and desiccation tolerance in Heterorhabditis bacteriophora through cross-breeding of tolerant strains and successive genetic selection. Biological Control, 55: 511–521.
 
Nguyen K.B., Smart G.C. Jr (1995): Morphometrics of infective juveniles of Steinernema spp. and Heterorhabditis bacteriophora (Nemata: Rhabditida). Journal of Nematology, 27: 206–212.
 
Mbata George, Shapiro-Ilan David, Nguyen Khuong (2008): Heterorhabditis georgiana n. sp. (Rhabditida: Heterorhabditidae) from Georgia, USA. Nematology, 10, 433-448 https://doi.org/10.1163/156854108783900276
 
Nielsen O., Philipsen H. (2005): Susceptibility of Meligethes spp. and Dasyneura brassicae to entomopathogenic nematodes during pupation in soil. Biological Control, 50: 623–634.
 
Poinar G.O. Jr (1990): Taxonomy and biology of Steinernematidae and Heterorhabditidae. In: Gaugler R., Kaya H.K. (eds): Entomopathogenic Nematodes in Biological Control. Boca Raton, CRC Press: 23–61.
 
Powell Jeff R, Webster John M (2004): Interguild antagonism between biological controls: impact of entomopathogenic nematode application on an aphid predator, Aphidoletes aphidimyza (Diptera: Cecidomyiidae). Biological Control, 30, 110-118 https://doi.org/10.1016/j.biocontrol.2003.09.006
 
Půža Vladimír, Mráček Zdeněk (2005): Seasonal dynamics of entomopathogenic nematodes of the genera Steinernema and Heterorhabditis as a response to abiotic factors and abundance of insect hosts. Journal of Invertebrate Pathology, 89, 116-122 https://doi.org/10.1016/j.jip.2005.04.001
 
Richardson P.N. (1987): Nematode parasites of mushroom flies: their use as biological control agents. In: Wuest P., Royse D., Beelaman R. (eds): Developments in Crop Science. Braunschweig, Elsevier, 10: 385–394.
 
Rohfritsch Odette (2008): Plants, gall midges, and fungi: a three-component system. Entomologia Experimentalis et Applicata, 128, 208-216 https://doi.org/10.1111/j.1570-7458.2008.00726.x
 
Saitou N., Nei M. (1987): The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406–425.
 
Shapiro-Ilan D.I., Han H., Dolinksi C. (2012): Entomopathogenic nematode production and application technology. Journal of Nematology, 44: 206–217.
 
Tanasković S., Milenković S. (2011): Open field surveys to evaluate the susceptibility of red raspberry genotypes to raspberry gall midge, Lasioptera rubi Schrank (Diptera, Cecidomyiidae). Integrated Plant Protection in Soft Fruits. IOBC/WPRS Bulletin, 70: 159–163. 
 
Tarasco E., Clausi M., Rappazzo G., Panzavolta T., Curto G., Sorino R., Oreste M., Longo A., Leone D., Tiberi R., Vinciguerra M.T., Triggiani O. (2015): Biodiversity of entomopathogenic nematodes in Italy. Journal of Helminthology, 89, 359-366 https://doi.org/10.1017/S0022149X14000194
 
Tóth T. (2006): Collection of entomopathogenic nematodes for biological control of insect pests. Journal of Fruit Ornamental and Plant Research, 14: 225–230.
 
Yegorenkova E., Yefremova Z. (2016): Notes on Lasioptera rubi (Schrank) (Diptera: Cecidomyiidae) and its larval parasitoids (Hymenoptera) on raspberries in Russia. Entomologica Fennica, 27: 15–22.
 
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