Erwinia mallotivora is the causal agent of papaya bacterial crown rot disease in Lampung Timur, Indonesia

Suharjo R., Oktaviana H.A., Aeny T.N., Ginting C., Wardhana R.A., Nugroho A., Ratdiana R. (2021): Erwinia mallotivora is the causal agent of papaya bacterial crown rot disease in Lampung Timur, Indonesia. Plant Protect. Sci., 57: 122–133.

download PDF

Sixteen bacterial strains showing oval, convex with a white colony colour were obtained from the water-soaked lesions on the petioles and leaves of infected papaya (cv. calina) collected from a papaya field in Lampung Timur, Indonesia. The pathogenicity test showed that all the strains produced the same symptoms with those found in the field. Four representative strains were then chosen for further investigation. The phenotypic characteristics revealed that the strains resembled Erwinia mallotivora. Two representative strains were further identified using a 16SrDNA sequence analysis. The result showed that the strains were placed within the group of the type strain and the reference strains of E. mallotivora. To the best of our knowledge, this is the first finding of E. mallotivora as the causal agent of papaya crown rot disease in Indonesia. Among the sixteen plants used for the host range test, the symptom was only observed on eggplants, but not on the other fifteen plant species.

Aeny T.N., Suharjo R., Ginting C., Hapsoro D., Niswati A. (2020): Characterization and host range assessment of Dickeya zeae associated with pineapple soft rot disease in East Lampung, Indonesia. Biodiversitas, 21: 587–595.
Amin N.M., Bunawan H., Redzuan R.A., Jaganath I.B.S. (2011): Erwinia mallotivora sp., a new pathogen of papaya (Carica papaya) in Peninsular Malaysia. International Journal of Molecular Sciences, 12: 39–45.
Campillo T., Luna E., Portier P., Fischer-LeSaux M., Lapitan N., Tisserat N.A., Leach J.E. (2015): Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia). International Journal of Systematic and Evolutionary Microbiology, 65: 3625–3633.
Cueva F.M.D., Waje A.F., Magdalita P.M., Justo V.P., Pathania N., Vawdrey L. (2017): Evaluation of inoculation techniques to screen for bacterial crown rot resistance in different breeding lines of Carica papaya. Journal of Plant Pathology, 99: 355–360.
Dickey R.S. (1979): Erwinia chrysanthemi: A comparative study of phenotypic properties of strains from several hosts and other Erwinia species. Phytopathology, 69: 324–329.
Dye D.W. (1968): A taxonomic study of the genus Erwinia. I. The "amylovora" group. New Zealand Journal of Science, 11: 590–607.
Fullerton R.A., Taufa L., Vanneste J.L., Yu J., Cornish D.A., Park D. (2011): First record of bacterial crown rot of papaya (Carica papaya) caused by an Erwinia papayae-like bacterium in the Kingdom of Tonga. Plant Disease, 95: 70. doi: 10.1094/PDIS-06-10-0455
Gardan L., Christen R., Achouak W., Prior P. (2004): Erwinia papayae sp. nov., a Pathogen of Papaya (Carica papaya). International Journal of Systematic and Evolutionary Microbiology, 54: 107–113.
Goto M. (1976): Erwinia mallotivora sp. nov., the causal organism of bacterial leaf spot of Mallotus japonicus. International Journal of Systematic Bacteriology, 26: 467–473.
Hauben L., Moore E.R.B., Vauterin L., Steenackers M., Mergaert J., Verdonck L., Swings J. (1998): Phylogenetic Position of Phytopathogens within the Enterobacteriaceae. Systematic and Applied Microbiology, 21: 384–397.
Hugh R., Leifson E. (1953): The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. Journal of Bacteriology, 66: 24–26.
Hall T.A. (1999): BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41:95–98.
Kim W.S., Gardan L., Rhim S.L., Geider K. (1999): Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). International Journal of Systematic Bacteriology, 49: 899–906.
King E.O., Ward M.K., Raney D.E. (1954): Two simple media for the demonstration of pyocyanin and fluorescin. The Journal of Laboratory and Clinical Medicine, 44: 301–307.
Kube M., Migdoll A.M, Müller I., Kuhl H., Beck A., ReinhardtR., Geider K. (2008): The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia. Environmental Microbiology, 10: 2211–2222.
Kumar S., Tamura K., Stecher G. (2016): MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870–1874.
Kwon S.W, Go S.J., Kang H.W., Ryu J.C., Jo J.K. (1997): Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences. International Journal of Systematic Bacteriology, 47: 1061–1067.
Lelliot R.A., Billing E., Hayward A.C. (1966): A determinative scheme for the fluorescent plant pathogenic pseudomonads. Journal of Applied Bacteriology, 29: 470–489.
Liu B., Luo J., Li W., Long X.F., Zhang Y.Q., Zeng Z.G., Tian Y.Q. (2016): Erwinia teleogrylli sp. nov., a bacterial isolate associated with a Chinese cricket. PLoS ONE, 11: e0146596. doi: 10.1371/journal.pone.0146596.t003
López M.M., Roselló M., Llop P., Ferrer S., Christen R., Gardan L. (2011): Erwinia piriflorinigrans sp. nov., a novel pathogen that causes necrosis of pear blossoms. International Journal of Systematic and Evolutionary Microbiology, 61: 561–567.
Magrou J. (1937): Dictionnaire des Bacteries Pathogenes pour l'Homme, les Animaux et les Plantes. In: Hauduroy P., Ehrdinger G., Urbain A., Guillot G., Magrou J. (eds): Paris, Masson & Cie.
Maktar N.H., Kamis S., Mohd Yusof F.Z., Hussain N.H. (2008): Erwinia papayae causing papaya dieback in Malaysia. Plant Pathology, 57: 774. doi: 10.1111/j.1365-3059.2008.01877.x
Matsuura T., Mizuno A., Tsukamoto T., Shimizu Y., Saito N., Sato S., Kikuchi S., Uzuki T., Azegami K., Sawada H. (2012): Erwinia uzenensis sp. nov., a novel pathogen that affects European pear trees (Pyrus communis L.). International Journal of Systematic and Evolutionary Microbiology, 62: 1799–1803.
Moore E.R.B., Mau M., Arnscheidt A., Bottger E.C., Hutson R.A., Collins M.D., van de Peer Y., de Wachter R., Timmis K.N. (1997): The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Systematic and Applied Microbiology, 19: 478–492.
Moretti C., Hosni T., Vandemeulebroecke K., Brady C., De Vos P., Buonaurio R., Cleenwerck I. (2011): Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi. International Journal of Systematic and Evolutionary Microbiology, 61: 2745–2752.
Nelson M.N., Alvarez A.M. (1980): Purple stain of Carica papaya. Plant Disease 64: 93–95.
Nhung P.H., Ohkusu K., Mishima N., Noda M., Shah M.M., Sun X., Hayashi M, Ezaki T. (2007): Phylogeny and species identification of the family Enterobacteriaceae based on dnaJ sequences. Diagnostic Microbiology and Infectious Disease, 58: 153–161.
Nishiyama K. (1978): The tentative plan of simple identification method of plant pathogenic bacteria. Shokubutsu Boeki, 32: 283–288.
Olabiyi A.M. (2010): First report of Erwinia stem canker of papaya (Carica papaya L.) in Nigeria. European Journal of Scientific Research, 46: 422–430.
Ramirez-Bahena M.H, Salazar S., Cuesta M.J., Tejedor C., Igual J.M., Fernandez-Pascual M., Peix A. (2016): Erwinia endophytica sp. nov., isolated from potato (Solanum tuberosum L.) stems. International Journal of Systematic and Evolutionary Microbiology, 66: 975–981.
Rezzonico F., Smits T.H.M., Born Y., Blom J., Frey J.E., Goesmann A., Cleenwerck I., de Vos P., Bonaterra A., Duffy B., Montesinos E. (2016): Erwinia gerundensis sp. nov., a cosmopolitan epiphyte originally isolated from pome fruit trees. International Journal of Systematic and Evolutionary Microbiology, 66: 1583–1592.
Rojas A.M., de los Rios J.E.G, Fischer-Le Saux M., Jimenez P., Reche P., Bonneau S., Sutra L., Mathieu-Daude F., McClelland M. (2004): Erwinia toletana sp. nov., associated with Pseudomonas savastanoi-induced tree knots. International Journal of Systematic and Evolutionary Microbiology, 54: 2217–2222.
Ryu E. (1940): A simple method of differentiation between grampositive and gram-negative organism without staining. Kitasato Archives of Experimental Medicine, 17: 58–63.
Schaad N.W., Jones J.B., Chun W. (2001): Laboratory Guide for Identification of Plant Pathogenic Bacteria. Minnesota, APS Press.
Semangun H. (2007): Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Yogyakarta, Gadjah Mada University Press.
Skrodenyte-Arbaciauskiene V., Radziute S., Stunzenas V., Buda V. (2012): Erwinia typographi sp. nov., isolated from bark beetle (Ips typographus) gut. International Journal of Systematic and Evolutionary Microbiology, 62: 942–948.
Society of American Bacteriologists (1957): Manual of Microbiological Methods. Conn H.J. (ed.). New York, McGraw-Hill Book Co.
Sproer C., Mendrock U., Swiderski J., Lang E., Stackebrandt E. (1999): The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae. International Journal of Systematic Bacteriology, 49: 1433–1438.
Suharjo R., Sawada H., Takikawa Y. (2014): Phylogenetic study of Japanese Dickeya spp. and development of new rapid identification methods using PCR-RFLP. Journal of General Plant Pathology, 80: 230–254.
Trujillo E.E., Schroth M.N. (1982): Two bacterial diseases of papaya trees caused by Erwinia species in the northern Mariana Islands. Plant Disease, 66: 116–120.
von Rant A. (1931): Uber eine Bakterienkrankheit bei dem Melonenbaume (Carica papaya L.) auf Java. Zentralblatt fürBakteriologie Parasitenkunde Infektionskrankheiten und Hygiene, 84: 481–487.
Webb R. (1985): Epidemiology and control of bacterial canker of papaya caused by an Erwinia sp. on St. Croix, US Virgin Islands. Plant Disease, 69: 305–309.
Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. (1991): 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697–703.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti