Effect of MgSO4 nutrition on Theobroma cacao L. susceptibility to Phytophthora megakarya infection

https://doi.org/10.17221/124/2016-PPSCitation:Emile M., Madina Banen C.V., Kusznierewicz B., Doungous O., Haouni S., Hawadak J., Niemenak N., Omokolo D.N. (2018): Effect of MgSO4 nutrition on Theobroma cacao L. susceptibility to Phytophthora megakarya infection. Plant Protect. Sci., 54: 74-82.
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A new strategy to reduce the severity of black pod disease (BPD) in T. cacao plants using MgSO4 nutrition was investigated. The dynamics of the tolerance to BPD of 18 susceptible T. cacao plantlets coming from the cross (♀SNK64 × ♂UPA14) was monitored during weekly (8 weeks) supply of MgSO4 into the soil. Prior to MgSO4 application, disease scores of the 18 plantlets (in six sets of three plantlets per set) were varying between 3.5 (susceptible) and 5 (highly susceptible). After MgSO4 application, a substantial decrease in disease scores was observed compared to the control. The percentage of disease tolerance gain of plantlets versus MgSO4 supplied (0–2.96 g) presented a quasi-hyperbolic curve with asymptotic line corresponding to 60% (day 28) and 70% (day 56). Cysteine content was not significantly different between the six triplets before MgSO4 nutrition. On days 28 and 56 of MgSO4 supplementation, cysteine content presented a pattern similar to the tolerance gain of plantlet sets. The monitoring of glutathione content versus MgSO4 supplementation (compared to day 0) showed sigmoid (day 28) and hyperbolic (day 56) curves which were associated with defined mathematical laws determined by MALAB software. Negative and highly significant correlations were observed between disease scores, cysteine and glutathione contents in leaves while positive and highly significant correlations were observed between cysteine and glutathione contents in leaves. These data might mean that MgSO4 nutrition significantly improved the tolerance of T. cacao. The mechanism of tolerance improvement might be associated with the synthesis of sulphur-containing compounds (cysteine and glutathione) which might be directly or indirectly used by T. cacao against P. megakarya.

References:
Bloem E. (2004): Sulphur supply and infection with Pyrenopeziza brassicae influence L-cysteine desulphydrase activity in Brassica napus L.. Journal of Experimental Botany, 55, 2305-2312  https://doi.org/10.1093/jxb/erh236
 
Bloem E., Haneklaus S., Salac I., Wickenhäuser P., Schnug E. (2007): Facts and Fiction about Sulfur Metabolism in Relation to Plant-Pathogen Interactions. Plant Biology, 9, 596-607  https://doi.org/10.1055/s-2007-965420
 
Cooper R. M. (2004): Elemental sulphur as an induced antifungal substance in plant defence. Journal of Experimental Botany, 55, 1947-1953  https://doi.org/10.1093/jxb/erh179
 
Driver J.A., Kuniyuki A.H. (1984): In vitro propagation of paradox walnut rootstock. Horticulture Science, 19: 507–509.
 
Dubuis P.-H., Marazzi C., Stadler E., Mauch F. (2005): Sulphur Deficiency Causes a Reduction in Antimicrobial Potential and Leads to Increased Disease Susceptibility of Oilseed Rape. Journal of Phytopathology, 153, 27-36  https://doi.org/10.1111/j.1439-0434.2004.00923.x
 
Ellman George L. (1959): Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82, 70-77  https://doi.org/10.1016/0003-9861(59)90090-6
 
Gaitonde MK (1967): A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochemical Journal, 104, 627-633  https://doi.org/10.1042/bj1040627
 
Kataoka T. (2004): Root-to-Shoot Transport of Sulfate in Arabidopsis. Evidence for the Role of SULTR3;5 as a Component of Low-Affinity Sulfate Transport System in the Root Vasculature. PLANT PHYSIOLOGY, 136, 4198-4204  https://doi.org/10.1104/pp.104.045625
 
Minyaka E., Niemenak N., Issali E.A., Sangaré A., Omokolo N.D. (2010): Sulphur deplection altered somatic embryogenesis in Theobroma cacao L. Biochemical difference related to sulphur metabolism between embryogeneic and non embryogenic calli. African Journal of Biotechnology, 9: 5665–5675.
 
Minyaka Emile, Niemenak Nicolas, Fotso , Sangare Abdourahamane, Ndoumou Omokolo Denis (2008): Effect of MgSO4 and K2SO4 on somatic embryo differentiation in Theobroma cacao L.. Plant Cell, Tissue and Organ Culture, 94, 149-160  https://doi.org/10.1007/s11240-008-9398-5
 
Mou Zhonglin, Fan Weihua, Dong Xinnian (2003): Inducers of Plant Systemic Acquired Resistance Regulate NPR1 Function through Redox Changes. Cell, 113, 935-944  https://doi.org/10.1016/S0092-8674(03)00429-X
 
NfInn T. (2005): Cocoa production in Cameroon. In: Brooks K.N., Ffolliott P.F. (eds): Moving Agroforestry into the Mainstream. 9th North American Agroforestry Conference Proceedings, June 12–15, 2005, St. Paul, USA.
 
Nyassé S., Cilas C., Herail C., Blaha G. (1995): Leaf inoculation as an early screening test for cocoa (Theobroma cacao L.) resistance to Phytophthora black pod disease. Crop Protection, 14, 657-663  https://doi.org/10.1016/0261-2194(95)00054-2
 
Nyassé S., Efombagn M.I.B., Kébé B.I., Tahi M., Despréaux D., Cilas C. (2007): Integrated management of Phytophthora diseases on cocoa (Theobroma cacao L): Impact of plant breeding on pod rot incidence. Crop Protection, 26, 40-45  https://doi.org/10.1016/j.cropro.2006.03.015
 
Nyassé S, Efombagn Mousseni IB, Bouambi E, Ndoumbe-Nkeng M, Eskes AB (2003): Early selection for resistance toPhytophthora megakarya in local and introduced cocoa varieties in Cameroon. Tropical Science, 43, 96-102  https://doi.org/10.1002/ts.97
 
Pokou N.D., N’Goran J.A.K., Kébé I., Eskes A., Tahi M., Sangaré A. (2008): Levels of resistance to Phytophthora pod rot in cocoa accessions selected on-farm in Côte d’Ivoire. Crop Protection, 27, 302-309  https://doi.org/10.1016/j.cropro.2007.07.012
 
Rausch Thomas, Wachter Andreas (2005): Sulfur metabolism: a versatile platform for launching defence operations. Trends in Plant Science, 10, 503-509  https://doi.org/10.1016/j.tplants.2005.08.006
 
Rausch T., Gromes R., Liedschulte V., Müller I., Bogs J., Galovic V., Wachter A. (2007): Novel Insight into the Regulation of GSH Biosynthesis in Higher Plants. Plant Biology, 9, 565-572  https://doi.org/10.1055/s-2007-965580
 
Saito K. (2004): Sulfur Assimilatory Metabolism. The Long and Smelling Road. PLANT PHYSIOLOGY, 136, 2443-2450  https://doi.org/10.1104/pp.104.046755
 
Schnug E., Haneklaus S., Booth E., Walker K.C. (1995): Sulphur supply and stress resistance in oilseed rape. In: Rapeseed Today and Tomorrow. Proceedings 9th International Rapeseed Congress, July 4–7, 1995, Cambridge, UK: 229–231.
 
Ströher E., Dietz K.-J. (2006): Concepts and Approaches Towards Understanding the Cellular Redox Proteome. Plant Biology, 8, 407-418  https://doi.org/10.1055/s-2006-923961
 
Williams J. S., Cooper R. M. (2004): The oldest fungicide and newest phytoalexin - a reappraisal of the fungitoxicity of elemental sulphur. Plant Pathology, 53, 263-279  https://doi.org/10.1111/j.0032-0862.2004.01010.x
 
Williams J. S., Hall S. A., Hawkesford M. J., Beale M. H., Cooper R. M. (2002): Elemental Sulfur and Thiol Accumulation in Tomato and Defense against a Fungal Vascular Pathogen. PLANT PHYSIOLOGY, 128, 150-159  https://doi.org/10.1104/pp.010687
 
Zook M., Hammerschmidt R. (1997): Origin of the Thiazole Ring of Camalexin, a Phytoalexin from Arabidopsis thaliana. Plant Physiology, 113, 463-468  https://doi.org/10.1104/pp.113.2.463
 
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