Varied expression pattern of the small heat shock protein gene encoding HSP17.7 against UVA, UVB, Cu2+ and Zn2+ stresses in sunflower

https://doi.org/10.17221/125/2015-PPSCitation:Büyük İ., Aras S., Cansaran-Duman D. (2016): Varied expression pattern of the small heat shock protein gene encoding HSP17.7 against UVA, UVB, Cu2+ and Zn2+ stresses in sunflower. Plant Protect. Sci., 52: 99-106.
download PDF
Today, one of the main objectives of agricultural biotechnology area is to find the responsible genes involved in stress response and engineering these genes to improve the plant response mechanisms. Therefore the current study was conducted to gain an insight on the role of HSP17.7 gene, which is a member of sHsps family, in defence mechanism of sunflower (Helianthus annuus L. cv. Confeta –Turkish cultivar) treated with different doses of UVA and UVB (4, 8, 12 and 20 kJ/m2) and concentrations of copper (Cu2+) and zinc (Zn2+) (80, 160, 320, 640, and 1280 µM) heavy metals. Based on our data, it was observed that different doses of UVA and UVB irradiation resulted in increased levels of HSP17.7 mRNA in sunflower plants. The highest levels of these increases (8 and 12 kJ/m2 of UVA) were seen under UVA stress. In contrast to UV stress, only the Cu2+concentration of 1280 µM led to higher expression levels of HSP17.7 gene compared to the control. Besides this, the 1280 µM concentration of Zn2+ treatment was the peak point of increased HSP17.7 mRNA levels for all stress conditions with nearly 8 times more than in the control sample. Negative correlations were found between malondialdehyde (MDA) levels and expression levels of HSP17.7 gene in sunflower plants subjected to current abiotic stress conditions. This correlation might indicate that an effective defence mechanism was in action and it might be concluded that the HSP17.7 gene can be used for identification of cultivars tolerant to UV and high doses of Cu2+ and Zn2+ for molecular breeding studies in the near future. These findings provide evidence of the HSP17.7 gene contribution to abiotic stress response in sunflower and will be helpful for the next studies about stress tolerance improvement in sunflower plants.
References:
AHN YEH-JIN, ZIMMERMAN J. LYNN (2006): Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro. Plant, Cell and Environment, 29, 95-104 https://doi.org/10.1111/j.1365-3040.2005.01403.x
 
Al-Whaibi Mohamed H. (2011): Plant heat-shock proteins: A mini review. Journal of King Saud University - Science, 23, 139-150 https://doi.org/10.1016/j.jksus.2010.06.022
 
Boyer J. S. (1982): Plant Productivity and Environment. Science, 218, 443-448 https://doi.org/10.1126/science.218.4571.443
 
Casati Paula, Campi Mabel, Morrow Darren J, Fernandes John F, Walbot Virginia (2011): Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize. BMC Genomics, 12, - https://doi.org/10.1186/1471-2164-12-321
 
Chamseddine Mediouni, Wided Ben Ammar, Guy Houlné, Marie-Edith Chabouté, Fatma Jemal (2009): Cadmium and copper induction of oxidative stress and antioxidative response in tomato (Solanum lycopersicon) leaves. Plant Growth Regulation, 57, 89-99 https://doi.org/10.1007/s10725-008-9324-1
 
Cho Un-Haing, Seo Nam-Ho (2005): Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation. Plant Science, 168, 113-120 https://doi.org/10.1016/j.plantsci.2004.07.021
 
Conte C., Mutti I., Puglisi P., Ferrarini A., Regina G., Maestri E., Manmiroli N. (1998): DNA fingerprinting analysis by a PCR based method for monitoring the genotoxic effects of heavy metals pollution. Chemosphere, 37, 2739-2749 https://doi.org/10.1016/S0045-6535(98)00317-8
 
Cottee Nicola S., Wilson Iain W., Tan Daniel K. Y., Bange Michael P. (2014): Understanding the molecular events underpinning cultivar differences in the physiological performance and heat tolerance of cotton (Gossypium hirsutum). Functional Plant Biology, 41, 56- https://doi.org/10.1071/FP13140
 
Ding Dong, Zhang Lifang, Wang Hang, Liu Zhijie, Zhang Zuxin, Zheng Yonglian (2009): Differential expression of miRNAs in response to salt stress in maize roots. Annals of Botany, 103, 29-38 https://doi.org/10.1093/aob/mcn205
 
Ferguson D. L., Guikema J. A., Paulsen G. M. (1990): Ubiquitin Pool Modulation and Protein Degradation in Wheat Roots during High Temperature Stress. PLANT PHYSIOLOGY, 92, 740-746 https://doi.org/10.1104/pp.92.3.740
 
Falcone Ferreyra Maria Lorena, Rius Sebastian, Emiliani Julia, Pourcel Lucille, Feller Antje, Morohashi Kengo, Casati Paula, Grotewold Erich (2010): Cloning and characterization of a UV-B-inducible maize flavonol synthase. The Plant Journal, 62, 77-91 https://doi.org/10.1111/j.1365-313X.2010.04133.x
 
Henle K J, Jethmalani S M, Nagle W A (1998): Stress proteins and glycoproteins (Review).. International Journal of Molecular Medicine, , - https://doi.org/10.3892/ijmm.1.1.25
 
Hodges D. Mark, DeLong John M., Forney Charles F., Prange Robert K. (1999): Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207, 604-611 https://doi.org/10.1007/s004250050524
 
Kim Nak Hyun, Hwang Byung Kook (2015): Pepper Heat Shock Protein 70a Interacts with the Type III Effector AvrBsT and Triggers Plant Cell Death and Immunity. Plant Physiology, 167, 307-322 https://doi.org/10.1104/pp.114.253898
 
Koo Hyun Jo, Park Soo Min, Kim Keun Pill, Suh Mi Chung, Lee Mi Ok, Lee Seong-Kon, Xinli Xia, Hong Choo Bong (2015): Small Heat Shock Proteins Can Release Light Dependence of Tobacco Seed during Germination. Plant Physiology, 167, 1030-1038 https://doi.org/10.1104/pp.114.252841
 
Kravets Elena A., Zelena Liubov B., Zabara Elena P., Blume Ya. B. (2012): Adaptation strategy of barley plants to UV-B radiation. Emirates Journal of Food and Agriculture, 24, 632-645 https://doi.org/10.9755/ejfa.v24i6.632645
 
Larkindale J., Vierling E. (2007): Core Genome Responses Involved in Acclimation to High Temperature. PLANT PHYSIOLOGY, 146, 748-761 https://doi.org/10.1104/pp.107.112060
 
Li H., Luo H., Li D., Hu T., Fu J. (2012): Antioxidant enzyme activity and gene expression in response to lead stress in perennial ryegrass. Journal of the American Society for Horticultural Science, 137: 80–85.
 
Liu Yunguo, Wang Xin, Zeng Guangming, Qu Dan, Gu Jiajia, Zhou Ming, Chai Liyuan (2007): Cadmium-induced oxidative stress and response of the ascorbate–glutathione cycle in Bechmeria nivea (L.) Gaud. Chemosphere, 69, 99-107 https://doi.org/10.1016/j.chemosphere.2007.04.040
 
Livak Kenneth J., Schmittgen Thomas D. (2001): Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods, 25, 402-408 https://doi.org/10.1006/meth.2001.1262
 
Marschner H. (1995): Mineral Nutrition of Higher Plants. 2nd Ed. San Diego, Academic Press.
 
Martínez-Lüscher J., Morales F., Delrot S., Sánchez-Díaz M., Gomés E., Aguirreolea J., Pascual I. (2013): Short- and long-term physiological responses of grapevine leaves to UV-B radiation. Plant Science, 213, 114-122 https://doi.org/10.1016/j.plantsci.2013.08.010
 
Millaleo R, Reyes- Diaz M, Ivanov A.G, Mora M.L, Alberdi M (2010): MANGANESE AS ESSENTIAL AND TOXIC ELEMENT FOR PLANTS: TRANSPORT, ACCUMULATION AND RESISTANCE MECHANISMS. Journal of soil science and plant nutrition, 10, 470-481 https://doi.org/10.4067/S0718-95162010000200008
 
Müller-Xing Ralf, Xing Qian, Goodrich Justin (2014): Footprints of the sun: memory of UV and light stress in plants. Frontiers in Plant Science, 5, - https://doi.org/10.3389/fpls.2014.00474
 
Pareek A., Sopory S.K., Bohnert H.J., Govindjee. (2010): Abiotic Stress Adaptation in Plants. Physiological, Molecular and Genomic Foundation. 1st Ed. Dordrecht, Springer.
 
Piraino F., Aina R., Palin L., Prato N., Sgorbati S., Santagostino A., Citterio S. (2006): Air quality biomonitoring: Assessment of air pollution genotoxicity in the Province of Novara (North Italy) by using Trifolium repens L. and molecular markers. Science of The Total Environment, 372, 350-359 https://doi.org/10.1016/j.scitotenv.2006.09.009
 
Pontin Mariela A, Piccoli Patricia N, Francisco Rita, Bottini Ruben, Martinez-Zapater Jose M, Lijavetzky Diego (2010): Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation. BMC Plant Biology, 10, 224- https://doi.org/10.1186/1471-2229-10-224
 
Pratt William B, Krishna Priti, Olsen Laura J (2001): Hsp90-binding immunophilins in plants: the protein movers. Trends in Plant Science, 6, 54-58 https://doi.org/10.1016/S1360-1385(00)01843-4
 
Rao G. M., Sumita P, Roshni M, Ashtagimatt M. N. (2005): Plasma antioxidant vitamins and lipid peroxidation products in pregnancy induced hypertension. Indian Journal of Clinical Biochemistry, 20, 198-200 https://doi.org/10.1007/BF02893070
 
Rizhsky Ludmila, Davletova Sholpan, Liang Hongjian, Mittler Ron (2004): The Zinc Finger Protein Zat12 Is Required for Cytosolic Ascorbate Peroxidase 1 Expression during Oxidative Stress in Arabidopsis. Journal of Biological Chemistry, 279, 11736-11743 https://doi.org/10.1074/jbc.M313350200
 
Ronde J.A. de, Mescht A., van der Cress W.A. (1993): Heat-shock protein synthesis in cotton is cultivar dependent. South African Journal of Plant and Soil, 10: 95–97.
 
Sairam R.K., Tyagi A. (2004): Physiology and molecular biology of salinity stress tolerance in plants. Current Science, 86: 407–421.
 
Sato Yutaka, Yokoya Sakiko (2008): Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17.7. Plant Cell Reports, 27, 329-334 https://doi.org/10.1007/s00299-007-0470-0
 
Savva Demetris (1998): Use of DNA Fingerprinting to Detect Genotoxic Effects. Ecotoxicology and Environmental Safety, 41, 103-106 https://doi.org/10.1006/eesa.1998.1674
 
Seo Jung Soo, Lee Young-Mi, Park Heum Gi, Lee Jae-Seong (2006): The intertidal copepod Tigriopus japonicus small heat shock protein 20 gene (Hsp20) enhances thermotolerance of transformed Escherichia coli. Biochemical and Biophysical Research Communications, 340, 901-908 https://doi.org/10.1016/j.bbrc.2005.12.086
 
Shinkle James R., Edwards Meredith C., Koenig Annalise, Shaltz Abigail, Barnes Paul W. (2010): Photomorphogenic regulation of increases in UV-absorbing pigments in cucumber (Cucumis sativus) and Arabidopsis thaliana seedlings induced by different UV-B and UV-C wavebands. Physiologia Plantarum, 138, 113-121 https://doi.org/10.1111/j.1399-3054.2009.01298.x
 
Soydam Aydin S., Büyük İ., Aras S. (2013): Relationships among lipid peroxidation, SOD enzyme activity, and SOD gene expression profile in Lycopersicum esculentum L. exposed to cold stress. Genetics and Molecular Research, 12, 3220-3229 https://doi.org/10.4238/2013.August.29.6
 
Sun Weining, Van Montagu Marc, Verbruggen Nathalie (2002): Small heat shock proteins and stress tolerance in plants. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1577, 1-9 https://doi.org/10.1016/S0167-4781(02)00417-7
 
Volkov Roman A., Panchuk Irina I., Mullineaux Phillip M., Schöffl Friedrich (2006): Heat stress-induced H2O2 is required for effective expression of heat shock genes in Arabidopsis. Plant Molecular Biology, 61, 733-746 https://doi.org/10.1007/s11103-006-0045-4
 
Wang Lili, Jacquet Michel, Renault Georges, Garreau Hervé (2004): Stress induces depletion of Cdc25p and decreases the cAMP producing capability in Saccharomyces cerevisiae. Microbiology, 150, 3383-3391 https://doi.org/10.1099/mic.0.27162-0
 
Wu H.-C., Hsu S.-F., Luo D.-L., Chen S.-J., Huang W.-D., Lur H.-S., Jinn T.-L. (): Recovery of heat shock-triggered released apoplastic Ca2+ accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings. Journal of Experimental Botany, 61, 2843-2852 https://doi.org/10.1093/jxb/erq121
 
Zahur Muzna, Maqbool Asma, Irfan Muhammad, Barozai Muhammad Younas Khan, Qaiser Uzma, Rashid Bushra, Husnain Tayyab, Riazuddin Shiekh (2009): Functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using Agrobacterium-mediated transient assay. Molecular Biology Reports, 36, 1915-1921 https://doi.org/10.1007/s11033-008-9399-9
 
download PDF

© 2019 Czech Academy of Agricultural Sciences