Trichoderma atroviride enhances phenolic synthesis and cucumber protection against Rhizoctonia solani

https://doi.org/10.17221/126/2016-PPSCitation:Nawrocka J., Szczech M., Małolepsza U. (2018): Trichoderma atroviride enhances phenolic synthesis and cucumber protection against Rhizoctonia solani. Plant Protect. Sci., 54: 17-23.
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The treatment of cucumber plants with Trichoderma atroviride TRS25 (TRS25) provided protection against infection by Rhizoctonia solani. In plants inoculated with the pathogen, nontreated with Trichoderma, disease symptoms were observed on the roots, shoots, and leaves while in plants treated with TRS25 the spread of the disease was limited. The induction of systemic defence response in cucumber against R. solani infection seemed to be strongly related to the enhanced synthesis of phenolic compounds in plants. HPLC analysis indicated remarkable increases in the concentrations of 23 phenolics belonging to hydroxybenzoic acids, cinnamic acids, catechins, flavonols, flavons, and flavanons in the plants without systemic disease symptoms. We suggest that the accumulation of phenolic acids, flavonoids and de novo synthesis of catechins may strongly contribute to cucumber protection against R. solani.
References:
Anees Muhammad, Edel-Hermann Véronique, Steinberg Christian (2010): Build up of patches caused by Rhizoctonia solani. Soil Biology and Biochemistry, 42, 1661-1672 https://doi.org/10.1016/j.soilbio.2010.05.013
 
Asad S.A., Ali N., Hameed A., Khan S.A., Ahmad R., Bilal M., Shahzad M., Tabassum A. (2014): Biocontrol efficacy of different isolates of Trichoderma against soil borne pathogen Rhizoctonia solani. Polish Journal of Microbiology, 63 (1): 95–103.
 
Castellano G., Tena J., Torres F. (2012): Classification of phenolic compounds by chemical structural indicators and its relation to antioxidant properties of Posidonia oceanica (L.) Delile. MATCH Communications in Mathematical and in Computer Chemisty, 67: 231–250.
 
Contreras-Cornejo H.A., Macías-Rodríguez L., Beltrán-Peña E., Herrera-Estrella A., López-Bucio J. (2011). Trichoderma-induced plant immunity likely involves both hormonal- and camalexin-dependent mechanisms in Arabidopsis thaliana and confers resistance against necrotrophic fungus Botrytis cinerea. Plant Signaling & Behavior, 6: 1554–1563.
 
Gams W., Bissett J. (1998): Morphology and identification of Trichoderma. In: Kubicek C.P., Harman G.E. (eds): Trichoderma and Gliocladium. Basic Biology, Taxonomy and Genetics. London, Taylor & Francis: 3–34.
 
Ghassempour Alireza, Mollayi Saeed, Farzaneh Mohsen, Sharifi-Tehrani Abbas, Aboul-Enein Hassan Y. (2011): Variation of Catechin, epicatechin and their enantiomers concentrations before and after wheat cultivar-Puccinia triticina infection. Food Chemistry, 125, 1287-1290 https://doi.org/10.1016/j.foodchem.2010.10.028
 
GIRARDI FELIPE A., TONIAL FABIANA, CHINI SILVIA O., SOBOTTKA ANDRÉA M., SCHEFFER-BASSO SIMONE M., BERTOL CHARISE D. (2014): Phytochemical profile and antimicrobial properties of Lotus spp. (Fabaceae). Anais da Academia Brasileira de Ciências, 86, 1295-1302 https://doi.org/10.1590/0001-3765201420130220
 
Hermosa R., Viterbo A., Chet I., Monte E. (2011): Plant-beneficial effects of Trichoderma and of its genes. Microbiology, 158, 17-25 https://doi.org/10.1099/mic.0.052274-0
 
Kelebek Hasim, Selli Serkan, Canbas Ahmet, Cabaroglu Turgut (2009): HPLC determination of organic acids, sugars, phenolic compositions and antioxidant capacity of orange juice and orange wine made from a Turkish cv. Kozan. Microchemical Journal, 91, 187-192 https://doi.org/10.1016/j.microc.2008.10.008
 
Keski-Saari Sarita, Julkunen-Tiitto Riitta (2003): Resource allocation in different parts of juvenile mountain birch plants: effect of nitrogen supply on seedling phenolics and growth. Physiologia Plantarum, 118, 114-126 https://doi.org/10.1034/j.1399-3054.2003.00077.x
 
López-Bucio José, Pelagio-Flores Ramón, Herrera-Estrella Alfredo (2015): Trichoderma as biostimulant: exploiting the multilevel properties of a plant beneficial fungus. Scientia Horticulturae, 196, 109-123 https://doi.org/10.1016/j.scienta.2015.08.043
 
Mandal S.M., Chakraborty D., Dey S. (2010): Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signaling & Behavior, 5: 359–368.
 
Mierziak Justyna, Kostyn Kamil, Kulma Anna (2014): Flavonoids as Important Molecules of Plant Interactions with the Environment. Molecules, 19, 16240-16265 https://doi.org/10.3390/molecules191016240
 
Molina Anabel, Bueno Pablo, Marin Maria Carmen, Rodriguez-Rosales Maria Pilar, Belver Andres, Venema Kees, Donaire Juan Pedro (2002): Involvement of endogenous salicylic acid content, lipoxygenase and antioxidant enzyme activities in the response of tomato cell suspension cultures to NaCl. New Phytologist, 156, 409-415 https://doi.org/10.1046/j.1469-8137.2002.00527.x
 
Pannecoucque J., Van Beneden S., Höfte M. (2008): Characterization and pathogenicity of Rhizoctonia isolates associated with cauliflower in Belgium. Plant Pathology, 57, 737-746 https://doi.org/10.1111/j.1365-3059.2007.01823.x
 
POURCEL L, ROUTABOUL J, CHEYNIER V, LEPINIEC L, DEBEAUJON I (2007): Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends in Plant Science, 12, 29-36 https://doi.org/10.1016/j.tplants.2006.11.006
 
Rao G.S., Reddy N.N.R., Surekha C. (2015): Induction of plant systemic resistance in Legumes Cajanus cajan, Vigna radiata, Vigna mungo against plant pathogens Fusarium oxysporum and Alternaria alternata – a Trichoderma viride mediated reprogramming of plant defense mechanism. International Journal of Science and Research, 6: 4270–4280.
 
Samuels Gary J., Dodd Sarah L., Gams Walter, Castlebury Lisa A., Petrini Orlando (2017): Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94, 146-170 https://doi.org/10.1080/15572536.2003.11833257
 
Skoneczny Dominik, Oskiera Michał, Szczech Magdalena, Bartoszewski Grzegorz (2015): Genetic diversity of Trichoderma atroviride strains collected in Poland and identification of loci useful in detection of within-species diversity. Folia Microbiologica, 60, 297-307 https://doi.org/10.1007/s12223-015-0385-z
 
Szczech M., Witkowska D., Piegza M., Kancelista A., Małolepsza U., Gajewska E., Kowalska B. (2014): Selection system for beneficial microorganisms following Trichoderma example. In: 11th Conference of the European Foundation for Plant Pathology Healthy Plants – Healthy People. Sep 8–13, 2014, Kraków, Poland: 301.
 
Taheri Parissa, Tarighi Saeed (2011): A survey on basal resistance and riboflavin-induced defense responses of sugar beet against Rhizoctonia solani. Journal of Plant Physiology, 168, 1114-1122 https://doi.org/10.1016/j.jplph.2011.01.001
 
Treutter Dieter (2006): Significance of flavonoids in plant resistance: a review. Environmental Chemistry Letters, 4, 147-157 https://doi.org/10.1007/s10311-006-0068-8
 
Wu Ting, Zang Xixi, He Mengying, Pan Siyi, Xu Xiaoyun (2013): Structure–Activity Relationship of Flavonoids on Their Anti-Escherichia coli Activity and Inhibition of DNA Gyrase. Journal of Agricultural and Food Chemistry, 61, 8185-8190 https://doi.org/10.1021/jf402222v
 
Vogt Thomas (2010): Phenylpropanoid Biosynthesis. Molecular Plant, 3, 2-20 https://doi.org/10.1093/mp/ssp106
 
Yamamoto M., Nakatsuka S., Otani H., Kohmoto K., Nishimura S. (2000): (+)-Catechin Acts as an Infection-Inhibiting Factor in Strawberry Leaf. Phytopathology, 90, 595-600 https://doi.org/10.1094/PHYTO.2000.90.6.595
 
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