In vitro compatibility of entomopathogenic fungus, Cladosporium cladosporioides with three plant extracts

https://doi.org/10.17221/135/2021-PPSCitation:

Islam M.T., Haque M.A., Mahmud N.U., Gupta D.R., Islam T. (2022): In vitro compatibility of entomopathogenic fungus, Cladosporium cladosporioides with three plant extracts. Plant Protect. Sci., 58: 213–219.

download PDF

The in vitro compatibility of the entomopathogenic fungus, Cladosporium cladosporioides BOU1, with the crude extract of three plants viz. Calotropis gigantea, Vitex negundo and Carissa macrocarpa at a concentration of 1% and 2% was investigated. The compatibility of the fungal isolate with the plant extracts was calculated using the biological index formula from the germination percentage, radial growth and spore production of BOU1. The compatibility of the plant extracts with the fungal isolate was also assessed based on the protease and lipase activities of the treated fungus. Both concentrations of plant extracts did not significantly inhibit the germination percentage, radial growth and spore production of BOU1 in a dose-depended manner. The average mean values of the biological index for the three plant extracts with two concentrations were more than 98%. The analyses of the data with the compatibility index formula suggest that irrespective of concentration, all three plant extracts were compatible with BOU1. When the plant extracts were added to the fungal isolate, the protease and lipase activities of the fungal isolate were not significantly inhibited compared to the untreated control. The reduction in all the variables of fungal growth and the enzymatic activity was less than 10% and 20%, respectively. From these findings, we can conclude that the fungal biocontrol agent C. cladosporioides BOU1 is compatible with the investigated plant extracts in terms of the fungal growth and enzymatic activities.

References:
Alam M.A., Habib M.R., Nikkon F., Khalequzzaman M., Karim M.R. (2009): Insecticidal activity of root bark of Calotropis gigantea L. against Tribolium castaneum (Herbst). World Journal of Zoology, 4: 90–95.
 
Ali S., Farooqi M.A., Sajjad A., Ullah M.I., Qureshi A.K., Siddique B., Waheed W., Sarfraz M., Asghar A. (2018): Compatibility of entomopathogenic fungi and botanical extracts against the wheat aphid, Sitobion avenae (Fab.) (Hemiptera: Aphididae). Egyptian Journal of Biological Pest Control, 28: 97. doi: 10.1186/s41938-018-0101-9 https://doi.org/10.1186/s41938-018-0101-9
 
Alves S.B., Moino A. Jr., Almeida J.E.M. (1998): Produtos fitossanitarios entomopatogenos. In: Alves S.B. (ed.). Controle Microbiano de Insetos. Piracicaba, FEALQ: 217–238. Portuguese.
 
Asi M.R., Bashir M.H., Afzal M., Ashfaq M., Sah S.T. (2010): Compatibility of entomopathogenic fungi, Metarhizium anisopliae and Paecilomyces fumosoroseus with selective insecticides. Pakistan Journal of Botany, 42: 4207–4214.
 
Baky N.F.A., Salam A.H.A. (2003): Natural incidence of Cladosporium spp. as a biocontrol agent against whiteflies and aphids in Egypt. Journal of Applied Entomology, 127: 228–235. https://doi.org/10.1046/j.1439-0418.2003.00662.x
 
Baltazar M.T., Dinis-Oliveira R.J., Bastos M.D.L., Tsatsakis A.M., Duarte J.A., Carvalho F. (2014): Pesticides exposure as etiological factors of Parkinson’s disease and other neurodegenerative disease – A mechanistic approach. Toxicology Letters, 230: 85–103. https://doi.org/10.1016/j.toxlet.2014.01.039
 
Bedini S., Sarrocco S., Baroncelli R., Vannacci G., Conti B. (2018): Pathogenic potential of Beauveria pseudobassiana as bioinsecticide in protein baits for the control of the medfly Ceratitis capitata. Bulletin of Insectology, 71: 31–38.
 
Castellanos-Moguel J., González-Barajas M., Mier T., Reyes-Montes Mdel R., Aranda E., Toriello C. (2007): Virulence testing and extracellular subtilisin-like (Pr1) and tripsina-like (Pr2) activity during propagule production of Paecilomyces fumosoroseus isolates from whiteflies (Homoptera: Aeyrodidae). Revista Iberoamericana de Micología, 24: 62–68. https://doi.org/10.1016/S1130-1406(07)70016-5
 
Castiglioni E., Vendramin J.D., Alves S.B. (2003): Compatibility between Beauveria bassiana and Metarhizium anisopliae with Nimkol-L in the control of Heterotermes tenuis. Manejo Integrado de Plagas y Agroecología, 69: 38–44.
 
Chandrasekaran T., Thyagarajan A., Santhakumari P.G., Pillai A.K.B., Krishnan U.M. (2019): Larvicidal activity of essential oil from Vitex negundo and Vitex trifoliaon dengue vector mosquito Aedes aegypti. Journal of the Brazilian Society of Tropical Medicine, 52: e20180459. doi: 10.1590/0037-8682-0459-2018 https://doi.org/10.1590/0037-8682-0459-2018
 
Chidawanyika F., Mudavanhu P., Nyamukondiwa C. (2012): Biologically based methods for pest management in agriculture under changing climates: Challenges and future directions. Insects, 3: 1171–1189. https://doi.org/10.3390/insects3041171
 
Gao T., Wang Z., Huang Y., Keyhani N.O., Huang Z. (2017): Lack of resistance development in Bemisia tabaci to Isaria fumosorosea after multiple generations of selection. Scientific Reports, 7: 42727. doi: 10.1038/srep42727 https://doi.org/10.1038/srep42727
 
Goettel M.S., Johnson D.L. (1994): Environmental impact and safety of fungal biocontrol agents. In: Lomer C.J., Prior C. (eds). Biological Control of Locusts and Grasshoppers. Wallingford, CAB International/IITA: 356–361.
 
Habib M.R., Karim M.R. (2016): Chemical characterization and insecticidal activity of Calotropis gigantea L. flower extract against Tribolium castaneum (Herbst). Asian Pacific Journal of Tropical Disease, 6: 996–999. https://doi.org/10.1016/S2222-1808(16)61171-4
 
Haridasan P., Gokuldas M., Ajaykumar A.P. (2017): Antifeedant effects of Vitex negundo L. leaf extracts on the stored product pest, Tribolium castaneum H. (Coleoptera: Tenebrionidae). International Journal of Pharmacy and Pharmaceutical Sciences, 9: 17–22. https://doi.org/10.22159/ijpps.2017v9i3.15600
 
Hernandez-Trejo A., Estrada-Drouaillet B., Lopez-Santillan J.A., Rios-Velasco C., Varela-Fuentes S.E., Rodriguez-Herrera R., Osorio-Hernandez E. (2019): In vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperda. International Journal of Experimental Botany, 88: 47–54.
 
Islam T., Gupta D.R., Surovy M.Z., Mahmud N.U., Mazlan N., Islam T. (2019): Identification and application of a fungal biocontrol agent Cladosporium cladosporioides against Bemisia tabaci. Biotechnology and Biotechnological Equipment, 33: 1698–1705.  https://doi.org/10.1080/13102818.2019.1695541
 
Isman M.B. (2006): Botanical insecticides, deterrents, and repellents in modern agricultural and an increasingly regulated world. Annual Review of Entomology, 51: 45–56. https://doi.org/10.1146/annurev.ento.51.110104.151146
 
Jaber L.R., Araj S.E., Qasem J.R. (2018): Compatibility of endophytic fungal entomopathogens with plant extracts for the management of sweetpotato whitefly Bemesia tabaci Gennadius (Homoptera: Aleyrodidae). Biological Control, 17: 164–171. https://doi.org/10.1016/j.biocontrol.2017.11.009
 
Kogan M. (1998): Integrated pest management: Historical perspectives and contemporary developments. Annual Review of Entomology, 43: 243–270. https://doi.org/10.1146/annurev.ento.43.1.243
 
Mandepudi D., Ravuru B.K., Mandepudi B. (2012): Experimental investigation for pest resistant properties of Calotropis gigantea. International Journal of Pharmaceuticals Application, 3: 380–386.
 
Mohan M.C., Narasimha P., Reddy N.P., Devi U.K., Kongara R., Sharma H.C. (2007): Growth and insect assays of Beauveria bassiana with neem to test their compatibility and synergism. Biocontrol Science and Technology, 17: 1059–1069.  https://doi.org/10.1080/09583150701714551
 
Mohmed A.S. (2019): Evaluation of the crude phenolic and terpenoid extracts of Carissa macrocarpa against Aphis fabae Scopoli (Hemiptera: Aphididae) in-vitro. Journal of Biopestiside, 12: 72–75.
 
Nana P., Ekesi S., Nchu F., Maniania N.K. (2016): Compatibility of Metarhizium anisopliae with Calpurnia aurea leaf extracts and virulence against Rhipicephalus pulchellus. Journal of Applied Entomology, 140: 590–597.  https://doi.org/10.1111/jen.12289
 
Purwar J.P., Sachan G.C. (2006): Synergistic effect of entomogenous fungi on some insecticides against Bihar hairy caterpillar Spilarctia oblique (Lepidoptera: Arctiidae). Microbiological Research, 161: 38–42.  https://doi.org/10.1016/j.micres.2005.04.006
 
Rajendran S., Sriranjini V. (2008): Plant products as fumigants for stored-product insect control. Journal of Stored Product Research, 44: 126–135.  https://doi.org/10.1016/j.jspr.2007.08.003
 
Regnault-Roger C., Philogène B.J.R. (2008): Past and current prospects for the use of botanicals and plant allelochemicals in integrated pest management. Pharmaceutical Biology, 46: 41–52. https://doi.org/10.1080/13880200701729794
 
Ribeiro L.P., Blume E., Bogorni P.C., Dequech S.T.B., Brand S.C., Junges E. (2012): Compatibility of Beauveria bassiana commercial isolate with botanical insecticides utilized in organic crops in southern Brazil. Biological Agriculture and Horticulture, 28: 1–18.  https://doi.org/10.1080/01448765.2012.735088
 
Ribeiro L.D.P., Mota L.H.C., D’alessandro C.P., Vendramim J.D., Delalibera Júnior I. (2014): In vitro compatibility of an acetogenin-based bioinsecticide with three species of entomopathogenic fungi. Florida Entomologist, 97: 1395–1403. https://doi.org/10.1653/024.097.0414
 
Rossi-Zalaf L.S., Alves S.B., Lopes R.B., Silveira-Neto S., Tanzini M.R. (2008): Interacao de microrganismos com outros agentes de controle de pragas e doencas. In: Alves S.B., Lopes R.B. (eds). Controle Microbiano de Pragasna America Latina: Avancos e Desafios. Piracicaba, FEALQ: 279–302. Portuguese.
 
Roubos C.R., Rodriguez-Saona C., Isaacs R. (2014): Mitigating the effects of insecticides on arthropod biological control at field and landscape scales. Biological Control, 75: 28–38. https://doi.org/10.1016/j.biocontrol.2014.01.006
 
Roy H.E., Pell J.K. (2000): Interactions between entomopathogenic fungi and other natural enemies: Implications for biological control. Biocontrol Science and Technology, 10: 737–752. https://doi.org/10.1080/09583150020011708
 
Sahayaraj K., Namasivayam S.K.R., Rathi J.M. (2011): Compatibility of entomopathogenic fungi with extracts of plants and commercial botanicals. African Journal of Biotechnology, 10: 933–938.
 
Schumacher V., Poehling H.M. (2012): In vitro effect of pesticides on the germination, vegetative growth, and conidial production of two strains of Metarhizium anisopliae. Fungal Biology, 116: 121–132. https://doi.org/10.1016/j.funbio.2011.10.007
 
Serebrov V.V., Khodyrev V.P., Gerber O.N., Tsvetkova V.P. (2005): Perspectives of combined use of entomopathogenic fungi and chemical insecticides against Colorado beetle (Leptinotarsa decemlineata). Mikologiya i Fitopatologiya, 39: 89–98.
 
Shahid A.A., Rao A.Q., Bakhsh A., Husnain T. (2012): Entomopathogenic fungi as biological controllers: New insights into their virulence and pathogenicity. Archive of Biological Sciences, 64: 21–42.  https://doi.org/10.2298/ABS1201021S
 
Srivastava S.K., Attri B.L., Hema P. (2006): Indigenous wisdom for the use of giant weed in disease and pest management. Indian Journal of Traditional Knowledge, 5: 83–86.
 
Wisuda N.L., Harjaka T., Prasetyaningrum I.D. (2019): Compatibility between Beauveria bassiana (Bals.) and neem extract against brown planthopper (Nilaparvata lugens Stal.). Ilmu Pertanian, 4: 9–14.
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti