Amino acid changes during the early stages of tomato wilt disease (Verticillium albo-atrum)

https://doi.org/10.17221/136/2020-PPSCitation:

Dixon G.R. (2021): Amino acid changes during early stages of tomato wilt disease (Verticillium albo-atrum). Plant Protect. Sci., 57: 140–147.

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Soil-borne pathogens such as Verticillium species, invade into the roots of many herbaceous and woody hosts.  The xylem environment supplies these pathogens with a continuous flow of nitrogen-rich nutrition. Detailed quantitative increases in amino acids in the stems, petioles, leaflets and roots of young tomato plants infected with Verticillium. albo-atrum the causal agent of wilt disease, are described in this paper for the first time. Results focus in particular on the vascular environment prior to the emergence of visual symptoms. Total amino acid concentrations in infected stems and petioles increased substantially at 144 and 216 h after inoculation. This effect was evident in leaflets at 216 h after inoculation. By 216 h most amino acid concentrations were substantially increased in stems, petioles and leaflets of infected plants relative to healthy controls. Earlier at 144 h in stems substantial increases were recorded for aspartic acid, threonine, serine, glutamic acid, glycine and ethanolamine. A similar picture emerged for petioles with the addition of increases in proline but not glycine. Amino acids increasing substantially in infected leaflets at 216 h were aspartic acid, glutamic acid and ethanolamine. In the infected roots there was relatively little difference in amino acid concentrations relative to healthy controls with the particular exceptions of proline and ethanolamine. By 18 days (432h), when visual symptoms were well advanced marked increases in amino acid concentrations were found for threonine, serine, α-alanine, valine, methionine, iso-leucine, leucine, tyrosine, ethanolamine, ornithine, lysine, histidine and arginine.

References:
Courtinho B.G., Mevers E., Schaefer A.L., Pelletier D.A., Harwood C.S., Clardy J., Greenberg E.P. (2018): A plant-responsive bacterial-signalling system senses an ethanolamine derivative. Proceedings of the National Academy of Sciences, 115: 9785–9790. https://doi.org/10.1073/pnas.1809611115
 
Dixon G.R. (1971a): Effect of root disturbance on the amino acid concentrations of young tomato leaflets. Plant and Soil, 34: 531–534. https://doi.org/10.1007/BF01372809
 
Dixon G.R. (1971b): Automated analysis of the amino acid content of tomatoes infected with Verticillium albo-atrum. Laboratory Practice, 20: 940–942.
 
Dixon G.R., Pegg G.F. (1972): Changes in amino acid content of tomato xylem sap following infection with strains of Verticillium albo-atrum. Annals of Botany, 36: 147–154. https://doi.org/10.1093/oxfordjournals.aob.a084567
 
Duncan D.R., Himelick E.B. (1987): The effects of amino acids on the growth and sporulation of Verticillium dahlia. Canadian Journal of Botany, 65: 1299–1302.  https://doi.org/10.1139/b87-182
 
Jiang Y.R., Fang P.W., Zhu S.J., Ji D.F. (2005): Relationship of Verticillium wilt resistance with plant anatomical structure and biochemical metabolism in upland cotton. Acta Agronomica Sinica, 31: 337–341.
 
Kawchuk L.M. (2010): Plant defense responses to pests and pathogens. Acta Horticulturae, 849: 259–268. https://doi.org/10.17660/ActaHortic.2010.849.30
 
Klosterman S.J., Atallah Z.K., Vallad G.E., Subbarao K.V. (2009): Diversity, pathogenicity, and management of Verticillium species. Annual Review of Phytopathology, 47: 39–62.  https://doi.org/10.1146/annurev-phyto-080508-081748
 
Koike S.T., Gladders P., Paulus A.O. (2007): Vegetable Diseases – A Colour Handbook. London, Manson Publishing.
 
Lowe-Powell T.M., Khokhani D., Allen C. (2018a): How Ralstonia solanacearum exploits and thrives in the flowing xylem environment. Trends in Microbiology, 26: 1–14. https://doi.org/10.1016/j.tim.2018.06.002
 
Lowe-Powell T.M., Hendrich C.G., Roepenack-Lahaye E., von Li B., Wu D., Mitra R., Dalsing B.L., Ricca P., Naidoo J., Cook D., Jancewicz A., Masson P., Thomma B., Lahaye T., Michael A.J., Allen C. (2018b): Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease. Environmental Microbiology, 20: 1330–1349. https://doi.org/10.1111/1462-2920.14020
 
NaNa L., Wang S.Y., Hong P.D., Xiao X.G., Yakun C.P., Guang L.F., Xia H.F. (2018): Mutation of key amino acids in the polygalacturonase-inhibiting proteins CkPGIP1 and GhPGIP1 improves resistance to Verticillium wilt in cotton. Plant Journal, 96: 546–561. https://doi.org/10.1111/tpj.14048
 
Nelson D.L. (2017): Lehninger, Principles of Biochemistry (7th ed.). New York, Worth Publishing.
 
Pegg G.F., Brady B.L (2002): Verticillium Wilts. Wallingford, CABI Publishing.
 
Peritore-Galve F.C., Matthew A., Tancos M.A., Smart C.D. (2020): Bacterial canker of tomato: Revisiting a global and economically damaging seedborne pathogen. Plant Disease, October 27. doi: 10.1094/PDIS-08-20-1732-FE https://doi.org/10.1094/PDIS-08-20-1732-FE
 
Qaiser S.H., Alyemeni H., Wani M.N., Pichtel A.S., Aqil Ahmad J. (2012): Role of proline under changing environments: A review. Plant Signaling and Behavior, 7: 1456–1466. https://doi.org/10.4161/psb.21949
 
Raitilack M.R., Minocha Lebar M.D., Rajasekaran K., Long S., Carter-Wientjes C., Mincocha S., Cary J.W. (2019): Contribution of maize polyamine and amino acid metabolism toward resistance against Aspergillus flavus infection and aflatoxin production. Frontiers in Plant Science, 10: 692. doi: 10.3389/fpls.2019.00692 https://doi.org/10.3389/fpls.2019.00692
 
Rontein D., Nishida I., Tashiro G., Yoshioka K., Wu W-I., Voelker D.R., Basset G., Hanson A.D. (2001): Plants synthesize ethanolamine by direct decarboxylation of serine using a pyridoxal phosphate enzyme. Journal of Biological Chemistry, 276: 35523–35529. https://doi.org/10.1074/jbc.M106038200
 
Sarhan A.R.T., Barna B., Kiraly Z. (1982): Effect of nitrogen nutrition on Fusarium wilt of tomato plants. Annals of Applied Biology, 101: 245–250.  https://doi.org/10.1111/j.1744-7348.1982.tb00819.x
 
Simons M., Permentier H.P., Weger L.A., de Wijffelman C.A., Lugtenberg B.J.J. (1997): Amino-acid synthesis is necessary for tomato root colonization by Pseudomonas fluorescens strain WCS365. Molecular Plant-Microbe Interactions, 10: 102–106.  https://doi.org/10.1094/MPMI.1997.10.1.102
 
Verbruggen N., Hermans C. (2008): Proline accumulation in plants: A review. Amino Acids, 35: 753–759.  https://doi.org/10.1007/s00726-008-0061-6
 
Zeier J. (2013): New insights into the regulation of plant immunity by amino acid metabolic pathways. Plant, Cell and Environment, 36: 2085–2103. https://doi.org/10.1111/pce.12122
 
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