Stable isotope labelling of Ceratitis capitata H., Agarwal M., Ren Y. (2019): Stable isotope labelling of Ceratitis capitata. Plant Protect. Sci., 55: 54-60.
download PDF

The use of stable isotopes to label an insect species, the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephidae) (medfly) was investigated. Labelling allows mating and life history characteristics to be investigated experimentally. 13C6-glucose was incorporated into the diet of medflies at various stages of development by adding it to larval media or providing adults with sugar water. Data was collected from egg hatching until the death of adults. The results show that stable isotopes successfully labelled medflies in laboratory conditions. There were significant differences between labelled and unlabelled treatments in terms of eggs hatching rates, larval development, pupae emergence, adult survival, and mating behaviour. Labelling during larval development, and combined labelling at the larval and adult stages, resulted in detectable values. Labelling in the larval stage had no effect on mating behaviour, but that in the adult stage did. This study demonstrates that it is possible to label adult medflies and to detect the label after mating.

Dame David A., Schmidt Claude H. (1964): P32-Labeled Semen for Mosquito Mating Studies2. Journal of Economic Entomology, 57, 669-672
De Groot P.A. (2004): Handbook of Stable Isotope Analytical Techniques. South Africa, Elsevier: 704–719.
Dyck V.A., Hendrichs J., Robinson A.S. (2006): Sterile Insect Technique: Principles and Practice in Area-Wide Integrated Pest Management. Vienna, Springer: 17–22.
Enkerlin Walther, Lopez Liliana, Celedonio Hilario (1996): Increased Accuracy in Discrimination Between Captured Wild Unmarked and Released Dye-Marked Adults in Fruit Fly (Diptera: Tephritidae) Sterile Released Programs. Journal of Economic Entomology, 89, 946-949
Graham H. M., Mangum C. L. (1971): Larval Diets Containing Dyes for Tagging Pink Bollworm Moths Internally123. Journal of Economic Entomology, 64, 376-379
Hagler James R., Jackson Charles G. (2001): M ETHODS FOR M ARKING I NSECTS : Current Techniques and Future Prospects. Annual Review of Entomology, 46, 511-543
Hamer Gabriel L., Donovan Danielle J., Hood-Nowotny Rebecca, Kaufman Michael G., Goldberg Tony L., Walker Edward D. (2012): Evaluation of a Stable Isotope Method to Mark Naturally-Breeding Larval Mosquitoes for Adult Dispersal Studies. Journal of Medical Entomology, 49, 61-70
Hayes J. M. (2001): Fractionation of Carbon and Hydrogen Isotopes in Biosynthetic Processes. Reviews in Mineralogy and Geochemistry, 43, 225-277
Heinrich Katharina, Weaver Robert J, Bell Howard A (2012): Determining the source of house flies (Musca domestica) using stable isotope analysis. Pest Management Science, 68, 31-37
Helinski M. E. H., Hood-Nowotny R., Mayr L., Knols B. G. J. (2007): Stable isotope-mass spectrometric determination of semen transfer in malaria mosquitoes. Journal of Experimental Biology, 210, 1266-1274
Helinski M.E., Hood R.C., Gludovacz D., Mayr L., Knols B.G. (2008): A 15N stable isotope semen label to detect mating in the malaria mosquito Anopheles arabiensis Patton. Parasites & Vectors, 1: 19. doi: 10.1186/1756-3305-1-19
Hendricks D. E., Leal M. P., Robinson S. H., Hernandez N. S. (1971): Oil-Soluble Black Dye in Larval Diet Marks Adults and Eggs of Tobacco Budworm and Pink Bollworm1234. Journal of Economic Entomology, 64, 1399-1401
Hershey Anne E., Pastor John, Peterson Bruce J., Kling George W. (1993): Stable Isotopes Resolve the Drift Paradox for Baetis Mayflies in an Arctic River. Ecology, 74, 2315-2325
Hood-Nowotny Rebecca, Knols Bart G. J. (2007): Stable isotope methods in biological and ecological studies of arthropods. Entomologia Experimentalis et Applicata, 124, 3-16
Hood-Nowotny R., Mayr L., Knols B.G. (2006): Use of carbon-13 as a population marker for Anopheles arabiensis in a sterile insect technique (SIT) context. Malaria Journal, 5: 6. doi: 10.1186/1475-2875-5-6
Hood-Nowotny Rebecca, Watzka Margarete, Mayr Leo, Mekonnen Solomon, Kapitano Berisha, Parker Andrew (2011): Intrinsic and Synthetic Stable Isotope Marking of Tsetse Flies. Journal of Insect Science, 11, 1-14
Hood-Nowotny Rebecca, Harari Ally, Seth Rakesh K., Wee Suk Ling, Conlong Des E., Suckling David M., Woods Bill, Lebdi-Grissa Kaouthar, Simmons Gregory, Carpenter James E. (2016): Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs. Florida Entomologist, 99, 166-176
INGER RICHARD, BEARHOP STUART (2008): Applications of stable isotope analyses to avian ecology. Ibis, 150, 447-461
Jessup A., Dominiak B., Woods B., De Lima C., Tomkins A., Smallridge C. (2007): Area-wide management of fruit flies in Australia. In: Vreysen M.J.B., Robinson A.S., Hendirichs J. (eds): Area-Wide Control of Insect Pests. Dordrecht, Springer: 685–697.
Knols B.G., Njiru B.N., Mathenge E.M., Mukabana W.R., Beier J.C., Killeen G.F. (2002): MalariaSphere: A greenhouse-enclosed simulation of a natural Anopheles gambiae (Diptera: Culicidae) ecosystem in western Kenya. Malaria Journal, 1: 19.
Langellotto Gail A., Rosenheim Jay A., Williams Megan R. (2005): Enhanced Carbon Enrichment in Parasitoids (Hymenoptera): A Stable Isotope Study. Annals of the Entomological Society of America, 98, 205-213[0205:ECEIPH]2.0.CO;2
MACNEALE KATE H., PECKARSKY BARBARA L., LIKENS GENE E. (2005): Stable isotopes identify dispersal patterns of stonefly populations living along stream corridors. Freshwater Biology, 50, 1117-1130
Mahroof Rizana M. (2013): Stable isotopes and elements as biological markers to determine food resource use pattern by Lasioderma serricorne (Coleoptera: Anobiidae). Journal of Stored Products Research, 52, 100-106
Malausa T. (2005): Assortative Mating in Sympatric Host Races of the European Corn Borer. Science, 308, 258-260
Markow T. A., Anwar S., Pfeiler E. (2000): Stable isotope ratios of carbon and nitrogen in natural populations of Drosophila species and their hosts. Functional Ecology, 14, 261-266
Mastrangelo T., Walder J. (2011): Use of radiation and isotopes in insects. In: Singh N. (ed.): Radioisotopes – Applications in Bio-Medical Science. Rijeka, InTech: 67–92.
Merritt R W, Dadd R H, Walker E D (1992): Feeding Behavior, Natural Food, and Nutritional Relationships of Larval Mosquitoes. Annual Review of Entomology, 37, 349-374
Misell L.M., Holochwost D., Boban D., Santi N., Shefi S., Hellerstein M.K., Turek P.J. (2006): A Stable Isotope-Mass Spectrometric Method for Measuring Human Spermatogenesis Kinetics In Vivo. The Journal of Urology, 175, 242-246
Munro Andrew R, Gillanders Bronwyn M, Elsdon Travis S, Crook David A, Sanger Andrew C (2008): Enriched stable isotope marking of juvenile golden perch ( Macquaria ambigua ) otoliths. Canadian Journal of Fisheries and Aquatic Sciences, 65, 276-285
Silva Neto Alberto Moreira da, Santos Tatiana Rodrigues de Oliveira, Dias Vanessa Simões, Joachim-Bravo Iara Sordi, Benevides Leandro de Jesus, Benevides Clicia Maria de Jesus, Silva Mariangela Vieira Lopes, dos Santos Deise Cristina Campos, Virgínio Jair, Oliveira Givanildo Bezerra, Walder Julio Marcos Melges, Paranhos Beatriz Aguiar Jordão, Nascimento Antônio Souza do (2012): Mass-rearing of Mediterranean fruit fly using low-cost yeast products produced in Brazil. Scientia Agricola, 69, 364-369
O'Leary Marion H. (1988): Carbon Isotopes in Photosynthesis. BioScience, 38, 328-336
Pauli J. N., Ben-David M., Buskirk S. W., DePue J. E., Smith W. P. (2009): An isotopic technique to mark mid-sized vertebrates non-invasively. Journal of Zoology, 278, 141-148
Pimentel David (2005): ‘Environmental and Economic Costs of the Application of Pesticides Primarily in the United States’. Environment, Development and Sustainability, 7, 229-252
Ponsard S, Bethenod M -T, Bontemps A, Pélozuelo L, Souqual M -C, Bourguet D (2004): Carbon stable isotopes: a tool for studying the mating, oviposition, and spatial distribution of races of European corn borer, Ostrinia nubilalis , among host plants in the field. Canadian Journal of Zoology, 82, 1177-1185
Schroeder W. J., Mitchell W. C., Miyabara R. Y. (1974): Dye-induced Changes in Melon Fly Behavior13. Environmental Entomology, 3, 571-571
Scott Thomas W., Takken Willem, Knols Bart G. J., Boëte Christophe (2002): The Ecology of Genetically Modified Mosquitoes. Science, 298, 117-119
Silverman J., Selbach H. (1998): Feeding behavior and survival of glucose-averse Blattella germanica (Orthoptera: Blattoidea: Blattellidae) provided glucose as a sole food source. Journal of Insect Behavior, 11: 93–102.
Thomas Donald B., Epsky Nancy D., Serra Colmar A., Hall David G., Kendra Paul E., Heath Robert R. (2008): Ammonia Formulations and Capture of Anastrepha Fruit Flies (Diptera: Tephritidae). Journal of Entomological Science, 43, 76-85
Walker Edward D., Copeland Robert S., Paulson Sally L., Munstermann Leonard E. (1987): Adult Survivorship, Population Density, and Body Size in Sympatric Populations of Aedes triseriatus and Aedes hendersoni (Diptera: Culicidae). Journal of Medical Entomology, 24, 485-493
WILKINS E. E., SMITH S. C., ROBERTS J. M., BENEDICT M. (2007): Rubidium marking of Anopheles mosquitoes detectable by field-capable X-ray spectrometry. Medical and Veterinary Entomology, 21, 196-203
Young A. D. M., Downe A. E. R. (1979): Quantitative Assessment with Radiotracers of Sperm Transfer by Male Aedes Aegypti (Diptera: Culicidae). Journal of Medical Entomology, 15, 259-264
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti