Sensitivity of Sclerotinia sclerotiorum to strobilurin fungicides in Slovakiaóthová M., Hudec K., Tóth P. (2020): Sensitivity of Sclerotinia sclerotiorum to strobilurin fungicides in Slovakia. Plant Protect. Sci., 56: 13-17.
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Rapeseed isolates of Sclerotinia sclerotiorum (Lib.) de Bary 1884 from Nitra Region of Slovakia were investigated for their in vitro sensitivity to azoxystrobin and picoxystrobin; and determining the EC50 value. The growth of S. sclerotiorum was evaluated on PDA amended with the selected fungicide´s active ingredient at 4 different concentration – 0.08, 0.83, 8.33, and 83.30 ppm. The overall mean EC50 values for azoxystrobin and picoxystrobin were 2.73 ppm and 3.12 ppm respectively. Majority of isolates had a resistance factors up to 20, that suggests the shift in S. sclerotiorum population sensitivity towards the resistance.

Adams P.B., Ayers W.A. (1979): Ecology of Sclerotinia species. Phytopathology, 69: 896–899.
Attanayake R.N., Carte, P.A., Jiang D., del Río-Mendoza L., Chen W. (2013): Sclerotinia sclerotiorum populations infecting canola from China and the United States are genetically and phenotypically distinct. Phytopathology, 103: 750–761.
Bečka D., Šimka J., Prokinová E., Cihlář P., Mikšík V., Vašák J., Zukalová H. (2011): Possibilities of improvement of winter rapeseed protection (Brassica napus L.) against Sclerotinia (Sclerotinia sclerotiorum). In: GCIRC: 13th International Rapeseed Congress, abstract book. Prague, International Consultative Research Group on Rapeseed. Prague, Jun 05–09, 2011: 1274–1277.
Bradley C.A., Lamey H.A., Endres G.J., Henson R.A., Hanson B.K., McKay K.R., Halvorson M., LeGare D.G., Porter P.M. (2006): Efficacy of fungicides for control of Sclerotinia stem rot of canola. Plant disease, 90: 1129–1134.
Delp C. J., Dekker J. (1985): Fungicide resistance: definitions and use of terms. EPPO Bulletin, 15: 333–335.
Derbyshire M. C., Denton-Giles M. (2016): The control of sclerotinia stem rot on oilseed rape (Brassica napus): current practices and future opportunities. Plant Pathology, 65: 859–877.
Duan Y., Liu S., Ge C., Feng X., Chen C., Zhou M. (2012): In vitro inhibition of Sclerotinia sclerotiorum by mixtures of azoxystrobin, SHAM, and thiram. Pesticide biochemistry and physiology, 103: 101–107.
FRAC (2013): Fungicide Resistance Action Committee Pathogen Risk List. Fungicide Resistance Action Committee, Brussels, Belgium.
FRAC (2018): FRAC Code List 2013: Fungicides sorted by mode of action. Fungicide Resistance Action Committee. Available at (accessed Oct 17, 2018).
Hailstones D. (2011): Identification and monitoring of resistance in vegetable crops in Australia. [Final Report VG07119.] Sydney: Horticulture Australia Ltd. Available at (accessed November 11, 2018).
Huszár J. (2011): Occurrence of Sclerotinia sclerotiorum (Lib) de Bary and Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al. on Iva xanthiifolia Nutt. in Slovak Republic. Plant Protection Science, 47: 52–54.
Ishii H., Holloman D.W. (2015). Fungicide resistance in plant pathogens. Tokyo, Springer.
Leroux P., Gredt M., Leroch M., Walker A. S. (2010): Exploring mechanisms of resistance to respiratory inhibitors in field strains of Botrytis cinerea, the causal agent of gray mold. Applied and Environmental Microbiology, 76: 6615–6630.
Matysiak R., Jaworska G., Dinter A. (2011): Dupont working for improved disease control strategies in oilseed rape. In: GCIRC: 13th International Rapeseed Congress, abstract book. Prague, International Consultative Research Group on Rapeseed. Prague, Jun 05–09, 2011: 1158–1161.
Munoz C.L. (2016): Sensitivity of Sclerotinia sclerotiorum isolates from North Central US to azoxystrobin and boscalid. [Ph.D. Thesis.] North Dakota, North Dakota State University.
Pandey D.K., Tripathi N.N., Tripathi R.D., Dixit S.N. (1982): Fungitoxic and phytotoxic properties of the essential oil of Hyptis suaveolens/Fungitoxische und phytotoxische Eigenschaften des ätherischen Öis von Hyptis suaveolens. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz/Journal of Plant Diseases and Protection, 1: 344–349.
Spitzer T., Matušinsky P., Klemová Z., Kazda J. (2012): Effect of fungicide application date against Sclerotinia sclerotiorum on yield and greening of winter rape. Plant Protection Science, 48: 105–109.
ÚKSÚP (2019): List of authorized plant protection products and parallel products permitted for parallel trade. Bratislava: Available at
(accessed May 31, 2019).
Williams J. R., Stelfox D. (1980): Influence of farming practices in Alberta on germination and apothecium production of sclerotia of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 2: 169–172.
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