In vitro antibacterial activity of Magnolia tamaulipana against tomato phytopathogenic bacteria

Arredondo-Valdés R., Chacón-Hernández J.C., Reyes-Zepeda F., Hernández-Castillo F.D., Anguiano-Cabello J.C., Heinz-Castro R.T.Q., Mora-Ravelo S.G. (2020): In vitro antibacterial activity of Magnolia tamaulipana against tomato phytopathogenic bacteria. Plant Protect. Sci., 56: 268–274.

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The tomato (Solanum lycopersicum Linnaeus) is one of the most important vegetable crops in the world. Still, there are phytopathogenic bacteria that cause a decrease in the yield or can kill the plant, like Pseudomonas syringae pv. tomato (Pst), Xanthomonas vesicatoria (Xv), Clavibacter michiganensis subsp. michiganensis (Cmm), Ralstonia solanacearum (Rs ) and Agrobacterium tumefeciens (At). Synthetic chemical fungicides are primarily used to control plant pathogenic bacteria, but their rapid growth makes them resistant to control. This research work is aimed at assessing the in vitro antibacterial activity of the ethanolic extract of Magnolia tamaulipana Vazquez leaves against Rs, Pst, Xv, Cmm, and At, as well as obtaining information about this plant species' chemical composition. The extract inhibited the growth of the five phytopathogenic bacteria that were tested. The growth inhibition rate ranged between 8.22 and 100%. The inhibitory concentration, IC50(90), required to inhibit 50 (90%) of Pst, Xv, Cmm, and At bacterial growth, was 34.71 (39.62), 23.09 (441.88), 64.75 (176.73) and 97.72 (535.48) ppm, respectively. The phytochemical analysis detected the presence of phenols, tannins, terpenes, saponins. M. tamaulipana ethanolic extract has antimicrobial properties and it must be considered a new control agent.

Abiodun J., Efe-Imafidon A., Benson-Oluwafemi A., Ajibola-Aluko P. (2017): Efficacy of selected plant extracts in the management of tomato early blight disease caused by Alternaria solani. Asian Journal of Plant Pathology, 11: 48–52.
Ali A., Rakha M., Shaheen F.A., Srinivasan R. (2019): Resistance of certain wild tomato (Solanum spp.) accessions to Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) based on choice and no-choice bioassays. Florida Entomologist, 102: 544–548.
Altemimi A., Lakhssassi N., Baharlouei A., Watson D.G., Lightfoot D.A. (2017): Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6: 1–23.
Balestra G.M., Heydari A., Ceccarelli D., Ovidi, E., Quattrucci A. (2009): Antibacterial effect of Allium sativum and Ficus carica extracts on tomato bacterial pathogens. Crop Protection, 28: 807–811.
Baştaş K.K. (2015): Determination of antibacterial efficacies of plant extracts on tomato bacterial speck disease. The Journal of Turkish Phytopathology, 44: 1–10.
Chacón-Hernández J.C., Arredondo-Valdés R., Anguiano-Cabello J.C., Ordaz-Silva S., Hernández-Juárez A., Reyes-Zepeda F. (2019): Effect of Magnolia tamaulipana extract on egg laying and food intake of Tetranychus urticae (Acari: Tetranychidae). International Journal of Acarology, 46: 108–110.
Devatha C.P., Thalla A.K., Katte S.Y. (2016): Green synthesis of iron nanoparticles using different leaf extracts for treatment of domestic waste water. Journal of Cleaner Production, 46: 108–110.
Dieringer G., Cabrera L., Lara M., Loya L., Reyes-Castillo P. (1999): Beetle pollination and floral thermogenicity in Magnolia tamaulipana (Magnoliaceae). International Journal of Plant Sciences, 160: 64–71.
Escobar M.A., Dandekar, A.M. (2003): Agrobacterium tumefaciens as an agent of disease. Trends in Plant Science, 8: 380–386.
FAO – (2017): FAOSTAT Database on Production, Crops, FAO Statistics Division, Food and Agriculture Organization of the United Nations, Rome. Available at (accessed Jan 9, 2020).
Finney D.J. (1971): Probit Analysis. London: Cambridge University Press.
Jacobo-Salcedo M.R., González-Espindola L.A., Alonso-Castro A.J., González-Martínez M.R., Domínguez F., García-Carranca A. (2011): Antimicrobial activity and cytotoxic effects of Magnolia dealbata and its active compounds. Natural Product Communications, 6: 1121–1124.
Jasso de Rodríguez D., Trejo-González F.A., Rodríguez-García R., Díaz-Jiménez M.L.V., Sáenz-Galindo A., Hernández-Castillo F.D., Villarreal-Quintanilla J.A., Peña-Ramos F.M. (2015): Antifungal activity in vitro of Rhus muelleri against Fusarium oxysporum f. sp. lycopersici. Industrial Crops and Products, 75(part B): 150–158.
Moreno-Limón S., González-Solís L.N., Salcedo-Martínez S.M., Cárdenas-Avila M.L., Perales-Ramírez A. (2011): Efecto antifúngico de extractos de gobernadora (Larrea tridentata L.) sobre la inhibición in vitro de Aspergillus flavus y Penicillium sp. Polibotánica, 32: 193–205.
Ramírez-Reyes T., Flores-Estévez N., Luna-Rodríguez M., Noa-Carrazana J.C., Sánchez-Velásquez L.R., Trigos-Landa A. (2015a): Extractos crudos de Magnolia schiedeana Schltdl. para el control de bacterias fitopatógenas. Madera y Bosques, 21: 159–164.
Ramírez-Reyes T., Luna-Rodríguez M., Noa-Carrazana J.C., Díaz-Fleischera F., Sánchez-Velásquez L.R., Flores-Estévez N. (2015b): Influence of season and organ on antibacterial activity of Magnolia dealbata Zucc. against two phytopathogenic bacteria. Chemistry and Ecology, 31: 47–52.
Ribeiro-Da Luz B. (2006): Attenuated total reflectance spectroscopy of plant leaves: a tool for ecological and botanical studies. New Phytologist, 172: 305–318.
Sahgal G., Ramanathan S., Sasidharan S., Mordi M.N., Ismail S., Mansor S.M. (2009): Phytochemical and antimicrobial activity of Swietenia mahagoni crude methanolic seed extract. Tropical Biomedicine, 26: 274–279.
SAS Institute (2002): SAS/STAT User’s Guide. North Carolina, Cary.
Schaad W., Jones J.B., Chun W. (2001): Laboratory Guide for Identification of Plant Pathogenic Bacteria, 3rd Ed. St Paul, American Phytopathological Society.
Soltani J., Aliabadi A.A. (2013): Antibacterial effects of several plant extracts and essential oils on Xanthomonas arboricola pv. juglandis in vitro. Journal of Essential Oil Bearing Plants, 16: 461–468.
Vázquez-G .J.A. (1994): Magnolia (Magnoliaceae) in Mexico and Central America: a synopsis. Britonia, 46: 1–23.
Vásquez-Morales S.G., Flores-Estévez N., Sánchez-Velásquez L.F., Pineda-López M.R., Viveros-Viveros H., Díaz-Fleischer F. (2015): Bioprospecting of botanical insecticides: the case of ethanol extracts of Magnolia schiedeana Schltl. applied to a tephritid fruit fly Anastrepha ludens Loew. Journal of Entomology and Zoology Studies, 3: 1–5.
Usman H., Abdulrahman F.I., Usman A. (2009): Qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of Ficus thonningii (Moraceae). African Journal of Traditional, Complementary and Alternative Medicines, 6: 289–295.
Watanabe K., Ikegami F, Horie S. (2002): Introduction the genus Magnolia. In: Sarker S.D., Maruyama Y. (eds): Magnolia. The Genus Magnolia. New York, Taylor & Francis.
Ziv C., Zhao Z., Gao Y. G., Xia Y. (2018): Multifunctional roles of plant cuticle during plant-pathogen interactions. Frontier in Plant Science. 9: 1088. doi:
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