PCR-based diagnostic methods for ‘Candidatus Liberibacter solanacearum’ – Review

https://doi.org/10.17221/145/2018-PPSCitation:Mirmajlessi S.M., Sjölund M.J., Mänd M., Loiseau M., Ilardi V., Haesaert G., Karise R., Gottsberger R.A., Sumner-Kalkun J., Bertaccini A. (2019): PCR-based diagnostic methods for ‘Candidatus Liberibacter solanacearum’ – Review. Plant Protect. Sci., 55: 229-242.
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Candidatus Liberibacter solanacearum’ is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several databases were searched from the inception of the relevant literature up to August 2018. This review identified 53 studies that met all the inclusion criteria. The performance of the different methods was also compared, however due to data heterogeneity and insufficient evidence on the sensitivity of all assays used, a meta-analysis of the data was not possible. Nonetheless, the review indicates that the rtPCR designed to the 16S ribosomal RNA gene can be routinely employed as a fast, cost-effective, and reliable detection technique in diagnostic laboratories.

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