Reaction of transgenic plum cv. HoneySweet to the Plum pox virus after a severe infection of Monilinia sp. – short communication

https://doi.org/10.17221/152/2017-PPSCitation:Polák J., Neubauerová T., Komínek P., Kundu J.K. (2019): Reaction of transgenic plum cv. HoneySweet to the Plum pox virus after a severe infection of Monilinia sp. – short communication. Plant Protect. Sci., 55: 8-10.
download PDF

Resistance to Plum pox virus (PPV) in transgenic Prunus domestica L., clone C5 (cv. HoneySweet) was evaluated in a regulated field in the Czech Republic for fifteen years (2002–2016). PPV mild symptoms appeared in C5 trees only in several leaves situated close to the point of inoculum grafting up to 2010. No symptoms of PPV were observed in the years 2011–2013 and results of ELISA and RT-PCR detection tests were negative. In the twelfth year (2013), there was a severe unusual natural attack of plum trees by Monilinia sp. This Monilinia sp. attack occurred only one time – in 2013. There was no Monilinia sp. infection in 2002–2012 and in 2014–2016. Mild PPV symptoms reappeared in several leaves of transgenic plum trees in the next two years (2014–2015) and the presence of PPV was proved by DAS-ELISA and confirmed by RT-PCR.

References:
Polák J., Pívalová J., Jokeš M., Svoboda J., Scorza R., Ravelonandro M. (2005): Preliminary results of interactions of Plum pox (PPV), Prune dwarf (PDV), and Apple chlorotic leafspot (ACLSV) viruses with transgenic plants of plum, Prunus domestica L. clone C-5 growed in an open field. Phytopathologia Polonica, 36: 115–122.
 
Polák J., Kumar J., Krška B., Ravelonandro M. (2012): Biotech/GM crops in horticulture: Plum cv. HoneySweet resistant to Plum pox virus. Plant Protection Science, 48: S43–S48.
 
Polák Jaroslav, Kundu Jiban K, Krška Boris, Beoni Eva, Komínek Petr, Pívalova Jitka, Jarošová Jana (2017): Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease. Journal of Integrative Agriculture, 16, 516-522 https://doi.org/10.1016/S2095-3119(16)61491-0
 
Ravelonandro M., Briard P., Scorza R. (2001): SIGNIFICANT RESISTANCE OF TRANSGENIC PLUMS AGAINST THE FOUR SEROTYPES OF PLUM POX POTYVIRUS. Acta Horticulturae, , 431-435 https://doi.org/10.17660/ActaHortic.2001.550.64
 
Scorza Ralph, Ravelonandro Michel, Callahan AnnM., Cordts JohnM., Fuchs Marc, Dunez Jean, Gonsalves Dennis (1994): Transgenic plums (Prunus domestica L.) express the plum pox virus coat protein gene. Plant Cell Reports, 14, - https://doi.org/10.1007/BF00233291
 
Scorza Ralph, Callahan Ann, Dardick Chris, Ravelonandro Michel, Polak Jaroslav, Malinowski Tadeusz, Zagrai Ioan, Cambra Mariano, Kamenova Ivanka (2013): Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum—from concept to product. Plant Cell, Tissue and Organ Culture (PCTOC), 115, 1-12 https://doi.org/10.1007/s11240-013-0339-6
 
Sochor J., Krška B., Polák J., Juriková T. (2015): The influence of virus infections on antioxidant levels in the GM plum variety HoneySweet (Prunus domestica L.). Potravinárstvo, 9: 195–200.
 
download PDF

© 2018 Czech Academy of Agricultural Sciences