Naturally occurring recombinant isolate of Pea seed-borne mosaic virus – Short Communication

https://doi.org/10.17221/155/2018-PPSCitation:Navrátil M., Šafářová D. (2019): Naturally occurring recombinant isolate of Pea seed-borne mosaic virus – Short Communication. Plant Protect. Sci., 55: 167-171.
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Whole genome sequences of three Czech Pea seed-borne mosaic virus isolates belonging to P1 pathotype and causing different symptom intensity were obtained. Using RDP4 analysis the natural recombinant isolate PSB204CZ bearing two breakpoints in nucleotide positions 4053 and 6080 was identified. The isolate was composed of fragment 2028 nt in length partially covering CI and 6K2 regions of the minor parent (PSB262CZ) incorporated into the major parent (PSB194CZ). The results suggest that the observed recombination in CI-6K2 region is responsible for severity of developed symptoms. This observation detected for the first time natural recombination within PSbMV isolates of an important pathogen of leguminous plants.

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