Gamma irradiation has been shown to be an effective method of controlling plant bacterial and fungal pathogens, but data on its effect against plant viruses is limited. A mechanism for the inactivation of plant viruses by gamma irradiation has not been proposed. Gamma irradiation was evaluated for the inactivation of Cucumber green mottle mosaic virus (CGMMV) in Nicotiana tabacum plants. CGMMV infectivity decreased gradually in a dose-dependent manner, and the virus was completely inactivated at over 40 kGy. Transmission electron microscopy revealed that gamma irradiation disrupts the virion structure and degrades viral protein and genomic RNA, suggesting that damage to viral constituents is the mechanism by which gamma irradiation inactivates the virus.
Asselin Alain, Zaitlin Milton (1978): Characterization of a second protein associated with virions of tobacco mosaic virus. Virology, 91, 173-181
https://doi.org/10.1016/0042-6822(78)90365-3
Asurmendi S., Berg R.H., Smith T.J., Bendahmane M., Beachy R.N. (2007): Aggregation of TMV CP plays a role in CP functions and in coat-protein-mediated resistance. Virology, 366, 98-106
https://doi.org/10.1016/j.virol.2007.03.014
Bawden F. C., Kleczkowski A. (1953): The Behaviour of some Plant Viruses after Exposure to Ultraviolet Radiation. Journal of General Microbiology, 8, 145-156
https://doi.org/10.1099/00221287-8-1-145
Blank G (1995): Comparison of resistance of fungal spores to gamma and electron beam radiation. International Journal of Food Microbiology, 26, 269-277
https://doi.org/10.1016/0168-1605(94)00129-T
Chu Eun-Hee, Shin Eun-Jung, Park Hae-Jun, Jeong Rae-Dong (2015): Effect of gamma irradiation and its convergent treatment for control of postharvest Botrytis cinerea of cut roses. Radiation Physics and Chemistry, 115, 22-29
https://doi.org/10.1016/j.radphyschem.2015.05.042
Dickson J.S. (2001): Radiation inactivation of microorganisms. In: Molins R. (ed.): Food Irradiation: Principles and Applications. New York, Wiley-Interscience: 23–35.
Feng K., Divers E., Ma Y., Li J. (2011): Inactivation of a Human Norovirus Surrogate, Human Norovirus Virus-Like Particles, and Vesicular Stomatitis Virus by Gamma Irradiation. Applied and Environmental Microbiology, 77, 3507-3517
https://doi.org/10.1128/AEM.00081-11
Golemboski D. B., Lomonossoff G. P., Zaitlin M. (1990): Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus.. Proceedings of the National Academy of Sciences, 87, 6311-6315
https://doi.org/10.1073/pnas.87.16.6311
Jeong R.-D., Chu E.-H., Shin E.-J., Lee E.-S., Kwak Y.-S., Park H.-J. (2015): Antifungal effect of gamma irradiation and sodium dichloroisocyanurate against
Penicillium expansum on pears. Letters in Applied Microbiology, 61, 437-445
https://doi.org/10.1111/lam.12466
kim Kyoung-Hee, Yook Hong-Sun (2009): Effect of gamma irradiation on quality of kiwifruit (Actinidia deliciosa var. deliciosa cv. Hayward). Radiation Physics and Chemistry, 78, 414-421
https://doi.org/10.1016/j.radphyschem.2009.03.007
Kim S.M., Lee J.M., Yin K.O., Oh M.H., Park J.W., Kim K.H. (2003): Nucleotide sequences of two Korean isolates of Cucumber green mottle mosaic virus. Molecules and Cells, 16: 407–412.
Predmore Ashley, Sanglay Gabriel C., DiCaprio Erin, Li Jianrong, Uribe R.M., Lee Ken (2015): Electron beam inactivation of Tulane virus on fresh produce, and mechanism of inactivation of human norovirus surrogates by electron beam irradiation. International Journal of Food Microbiology, 198, 28-36
https://doi.org/10.1016/j.ijfoodmicro.2014.12.024
Rae Chris, Koudelka Kristopher J., Destito Giuseppe, Estrada Mayra N., Gonzalez Maria J., Manchester Marianne, Geraghty Robert J. (2008): Chemical Addressability of Ultraviolet-Inactivated Viral Nanoparticles (VNPs). PLoS ONE, 3, e3315-
https://doi.org/10.1371/journal.pone.0003315
Smolko Eduardo E., Lombardo Jorge H. (2005): Virus inactivation studies using ion beams, electron and gamma irradiation. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 236, 249-253
https://doi.org/10.1016/j.nimb.2005.04.055
Stewart E.M. (2001): Food irradiation chemistry. In: Molins R. (ed.): Food Irradiation: Principles and Applications. New York, Wiley-Interscience: 37–76.
Urban M., Motteram J., Jing H.-C., Powers S., Townsend J., Devonshire J., Pearman I., Kanyuka K., Franklin J., Hammond-Kosack K.E. (2011): Inactivation of plant infecting fungal and viral pathogens to achieve biological containment in drainage water using UV treatment. Journal of Applied Microbiology, 110, 675-687
https://doi.org/10.1111/j.1365-2672.2010.04917.x
Varveri C., Vassilakos N., Bem F. (2002): Characterization and detection ofCucumber green mottle mosaic virus in Greece. Phytoparasitica, 30, 493-501
https://doi.org/10.1007/BF02979754
