Assessing BactoMix 5 efficacy for clubroot control in naturally infested soil

Loit K., Kiiker R., Puidet P., Soonvald L., Põldmets M., Mänd M. (2021): Assessing BactoMix 5 efficacy for clubroot control in naturally infested soil. Plant Protect. Sci., 57: 14–20.

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The cultivation of cruciferous crops is threatened by extensive yield losses caused by the soil-borne pathogen Plasmodiophora brassicae Woronin, 1877. The objective of the study was to assess the potential of the bacterial product BactoMix 5 for the control of clubroot on a naturally infested soil in growth chamber trials using a P. brassicae-specific qPCR methodology. The results did not show a significant decrease in the P. brassicae in the soil nor a reduction of the disease symptoms on the plants. The native soil microbiota may have exhibited an antagonistic activity against the bacterial species from BactoMix 5 and evoked the poor effect of the product. Therefore, potential biological control agents should be tested with native soil microbiota and the regional production should be advanced to increase the product efficacy in the environment

Almquist C. (2016): Monitoring important soil-borne plant pathogens in Swedish crop production using real-time PCR. [PhD Thesis]. Upsala, Swedish University of Agricultural Sciences: 78.
CABI (2019): Invasive Species Compendium. Datasheet: Plasmodiophora brassicae (club root). Available at (accesed March 28, 2019).
Cao T., Rennie D.C., Manolii V.P., Hwang S.F., Falak I., Strelkov S.E. (2014): Quantifying re-sistance to Plasmodiophora brassicae in Brassica hosts. Plant Pathology, 63: 715–726.
Castlebury, L.A., Maddox J.V., Glawe D.A. (1994): A technique for the extraction and purifica-tion of viable Plasmodiophora brassicae resting spores from host root tissue. Mycologia, 86: 458–460.
Dixon G.R., Page L.V. (1998): Calcium and nitrogen eliciting alterations to growth and repro-duction of Plasmodiophora brassicae (clubroot). Acta Horticulturae, 459: 343–349.
Dixon G.R. (2009): The occurrence and economic impact of Plasmodiophora brassicae and clubroot disease. Journal of Plant Growth Regulation, 28: 194–202.
Donald C., Lawrence J.M., Porter I. (2004): Influence of particle size and application method on the efficacy of calcium cyanamide for control of clubroot of vegetable brassicas. Crop Protec-tion, 23: 297–303.
Donald C., Porter I. (2009): Integrated control of clubroot. Journal of Plant Growth Regulation, 28: 289–303.
Einhorn G., Bochow H., Huber J., Krebs B. (1991): Methodological studies on the detection of antagonists of the clubroot pathogen Plasmodiophora brassicae Wor. Archive für Phytopa-thologie und Pflanzenschutz, 27: 205–208.
Faggian R., Strelkov S.E. (2009): Detection and measurement of Plasmodiophora brassicae. Journal of Plant Growth Regulation, 28: 282–288.
Gao Y., Xu, G. (2014): Development of an effective nonchemical method against Plasmodiopho-ra brassicae on Chinese cabbage. International Journal of Agronomy, 2014: 1–5.
Guetsky R., Shtienberg D., Elad Y., Fischer E., Dinoor A. (2002): Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phyto-pathology, 92: 976–985.
Handelsman J., Stabb E.V. (1996): Biocontrol of soilborne plant pathogens. The Plant Cell, 8: 1855–1869.
He P., Cui W., Munir S., He P., Li X., Wu Y., Yang X., Tang P., He Y. (2019): Plasmodiophora brassicae root hair interaction and control by Bacillus subtilis XF-1 in Chinese cabbage. Biologi-cal Control, 28: 56–63.
Hwang S., Strelkov S.E., Feng J., Gossen B. D., Howard R.J. (2012): Plasmodiophora brassicae: a review of an emerging pathogen of the Canadian canola (Brassica napus) crop. Molecular Plant Pathology, 13: 105–113.
Kuginuki Y., Hiroaki Y., Hirai M. (1999): Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. spp. pe-kinensis). European Journal of Plant Pathology, 105: 327–332.
Kumar S. (2012): Biopesticides: a need for food and environmental safety. Journal of Biofertilizers and Biopesticides, 3: e107. doi: 10.4172/2155-6202.1000e107
Kurowski T.P., Majchrzak B., Kowalska E. (2009): The effectiveness of the biological control of clubroot (Plasmodiophora brassicae) in Brassicaceae plants. Phytopathologia, 52: 5–12.
Lahlali R., Peng G., Gossen B.D., McGregor L., Yu F.Q., Hynes R.K., Hwang S.F., McDonald M.R., Boyetchko S.M. (2013): Evidence that the biofungicide Serenade (Bacillus subtilis) sup-presses clubroot on canola via antibiosis and induced host resistance. Phytopathology, 103: 245–254.
Lengai G., Muthomi J. (2018): Biopesticides and their role in sustainable agricultural production. Journal of Biosciences and Medicines, 6: 7–41.
Mehnaz S. (2013): Secondary metabolites of Pseudomonas aurantiaca and their role in plant growth promotion. In: Arora N.K. (ed.): Plant Microbe Symbiosis: Fundamentals and Advances. New Delhi, Springer: 373–393.
O'Brien P. (2017): Biological control of plant diseases. Australasian Plant Pathology, 46: 293–304.
Pal K.K., McSpadden Gardener B. (2006): Biological control of plant pathogens. The Plant Health Instructor. doi: 10.1094/PHI-A-2006-1117-02
Panda A.K , Bisht S.S., DeMondal S., Senthil Kumar N., Gurusubramanian G., Panigrahi A.K. (2014): Brevibacillus as a biological tool: a short review. Antonie Van Leeuwenhoek, 105: 623–639.
Peng G., Lahlali R., Hwang S.F., Pageau D., Hynes R.K., Mc-Donald M.R., Gossen B.D., Strelkov S.E. (2014): Crop rotation, cultivar resistance, and fungicides/biofungicides for managing clubroot (Plasmodiophora brassicae) on canola. Canadian Journal of Plant Patholo-gy, 36: 99–112.
Rennie D.C, Manolii V.P., Cao T., Hwang S.F., Howard R.J., Strelkov S.E. (2011): Direct evi-dence of surface infestation of seeds and tubers by Plasmodiophora brassicae and quantification of spore load. Plant Pathology, 60: 811–819.
Říčařová V., Kazda J., Singh K., Ryšánek P. (2016): Clubroot caused by Plasmodiophora brassi-cae Wor.: a review of emerging serious disease of oilseed rape in the Czech Republic. Plant Pro-tection Science, 52: 71–86.
Ruijter J.M., Ramakers C., Hoogaars W.M. H., Karlen Y., Bakker O., van den Hoff M.J.B., Moorman A.F.M. (2009): Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Research, 37: e45. doi: 10.1093/nar/gkp045
SCANDAGRA (2019): BactoMix 5. Available at https:// (accessed March 20, 2019).
Singh H.B. (2014): Management of plant pathogens with microorganisms. Proceedings of the Indian National Science Academy, 2: 443–454.
Todero I., Confortin T.C., Luft L., Brun T., Ugalde G.A., de Almeida T.C., Arnemann J.A., Zabot G.L., Mazutti M.A. (2018): Formulation of a bioherbicide with metabolites from Phoma sp. Scientia Horticulturae, 241: 285–292.
Vinale F., Sivasithamparam K., Ghisalberti E., Marra R., Barbetti M.J., Li H., Woo S., Lorito M. (2008): A novel role for The Trichoderma–plant interaction Trichoderma secondary metabolites in the interactions with plants. Physiological and Molecular Plant Pathology, 72: 80–86.
Walker R., Powell A.A., Seddon B. (1998): Bacillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species. Journal of Applied Microbiology, 84: 794–801.
Wallenhammar A.C. (1996): Prevalence of Plasmodiophora brassicae in a spring oilseed rape growing area in central Sweden and factors influencing soil infestation levels. Plant Pathology, 45: 710–719.
Wallenhammar A., Almquist C., Söderström M., Jonsson A. (2012): In-field distribution of Plasmodiophora brassicae measured using quantitative real-time PCR. Plant Pathology, 61: 16–28.
Wallenhammar A.C., Gunnarson A., Hansson F., Jonsson A. (2016): Quantification of Plasmodiophora brassicae using a DNA-based soil test facilitates sustainable oilseed rape pro-duction. Plants (Basel), 5:21. doi: 10.3390/plants5020021
Xu X.-M., Jeffries P., Pautasso M., Jeger M.J. (2011): Combined use of biocontrol agents to manage plant diseases in theory and practice. Phytopathology, 101: 1024–1031.
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