Fusarium culmorum Tri genes and barley Hvugt13248 gene transcription in infected barley cultivars

https://doi.org/10.17221/21/2018-PPSCitation:Faltusová Z., Vaculová K., Pavel J., Svobodová I., Hajšlová J., Ovesná J. (2019): Fusarium culmorum Tri genes and barley Hvugt13248 gene transcription in infected barley cultivars. Plant Protect. Sci., 55: 172-180.
download PDF

The transcription activities of genes somehow associated with the mycotoxin deoxynivalenol (DON) biosynthesis, namely Fusarium Tri genes, and the barley gene coding for UDP-glycosyltransferase (HvUGT13248) on different genetic backgrounds were compared. Determining the amount of the pathogen DNA was used as a useful tool for evaluating the infestation of barley cultivars. Amounts of the pathogen DNA differed in six barley cultivars infected by F. culmorum. Transcription of HvUGT13248 was related to DON content in the samples. Low pathogenic infection and low DON content were accompanied by increased Fusarium Tri10 transcription in resistant cv. Amulet. This finding confirmed our recent results and makes us propose using this change as a possible marker of barley resistance against Fusarium.

References:
Bai G.-H., Plattner R., Desjardins A., Kolb F. (2001): Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding, 120, 1-6 https://doi.org/10.1046/j.1439-0523.2001.00562.x
 
Berthiller Franz, Krska Rudolf, Domig Konrad J., Kneifel Wolfgang, Juge Nathalie, Schuhmacher Rainer, Adam Gerhard (2011): Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. Toxicology Letters, 206, 264-267 https://doi.org/10.1016/j.toxlet.2011.08.006
 
Boutigny Anne-Laure, Barreau Christian, Atanasova-Penichon Vessela, Verdal-Bonnin Marie-Noëlle, Pinson-Gadais Laëtitia, Richard-Forget Florence (2009): Ferulic acid, an efficient inhibitor of type B trichothecene biosynthesis and Tri gene expression in Fusarium liquid cultures. Mycological Research, 113, 746-753 https://doi.org/10.1016/j.mycres.2009.02.010
 
Chrpová J., Šíp V., Štočková L., Stemberková L., Tvarůžek L. (2011): Resistance to Fusarium head blight in spring barley. Czech Journal of Genetics and Plant Breeding, 47, 58-63 https://doi.org/10.17221/129/2010-CJGPB
 
Demeke Tigst, Gräfenhan Tom, Clear Randy M., Phan Anh, Ratnayaka Indira, Chapados Julie, Patrick Susan K., Gaba Don, Lévesque C. André, Seifert Keith A. (2010): Development of a specific TaqMan® real-time PCR assay for quantification of Fusarium graminearum clade 7 and comparison of fungal biomass determined by PCR with deoxynivalenol content in wheat and barley. International Journal of Food Microbiology, 141, 45-50 https://doi.org/10.1016/j.ijfoodmicro.2010.04.020
 
Edwards Simon G. (2004): Influence of agricultural practices on fusarium infection of cereals and subsequent contamination of grain by trichothecene mycotoxins. Toxicology Letters, 153, 29-35 https://doi.org/10.1016/j.toxlet.2004.04.022
 
Faltusová Zuzana, Chrpová Jana, Salačová Linda, Džuman Zbyněk, Pavel Jozef, Zachariášová Milena, Hajšlová Jana, Ovesná Jaroslava (2015): Effect of Fusarium culmorum Tri Gene Transcription on Deoxynivalenol and D3G Levels in Two Different Barley Cultivars. Journal of Phytopathology, 163, 593-603 https://doi.org/10.1111/jph.12359
 
Foroud Nora, Eudes François (2009): Trichothecenes in Cereal Grains. International Journal of Molecular Sciences, 10, 147-173 https://doi.org/10.3390/ijms10010147
 
Gang G., Miedaner T., Schuhmacher U., Shollenberger M., Geiger H.H. (1998): Deoxynivalenol and nivalenol production by Fusarium culmorum isolates differing in aggressiveness toward winter rye. Ecology and Population Biology, 88: 879–884.
 
Güldener Ulrich, Seong Kye-Yong, Boddu Jayanand, Cho Seungho, Trail Frances, Xu Jin-Rong, Adam Gerhard, Mewes Hans-Werner, Muehlbauer Gary J., Kistler H. Corby (2006): Development of a Fusarium graminearum Affymetrix GeneChip for profiling fungal gene expression in vitro and in planta. Fungal Genetics and Biology, 43, 316-325 https://doi.org/10.1016/j.fgb.2006.01.005
 
Hajšlová J., Lancová K., Sehnalová M., Krplová A., Zachariášová M., Moravcová H., Nedělník J., Marková J., Ehrenbergerová J. (2008): Occurrence of trichothecene mycotoxins in cereals harvested in the Czech Republic. Czech Journal of Food Sciences, 25, 339-350 https://doi.org/10.17221/745-CJFS
 
Hou Rui, Jiang Cong, Zheng Qian, Wang Chenfang, Xu Jin-Rong (2015): The AreA transcription factor mediates the regulation of deoxynivalenol (DON) synthesis by ammonium and cyclic adenosine monophosphate (cAMP) signalling in Fusarium graminearum. Molecular Plant Pathology, 16, 987-999 https://doi.org/10.1111/mpp.12254
 
Jiang Cong, Zhang Chengkang, Wu Chunlan, Sun Panpan, Hou Rui, Liu Huiquan, Wang Chenfang, Xu Jin-Rong (2016): TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum. Environmental Microbiology, 18, 3689-3701 https://doi.org/10.1111/1462-2920.13279
 
Kumaraswamy Kenchappa G., Kushalappa Ajjamada C., Choo Thin M., Dion Yves, Rioux Sylvie (2011): Mass Spectrometry Based Metabolomics to Identify Potential Biomarkers for Resistance in Barley against Fusarium Head Blight (Fusarium graminearum). Journal of Chemical Ecology, 37, 846-856 https://doi.org/10.1007/s10886-011-9989-1
 
Leišová L., Kučera L., Chrpová J., Sýkorová S., Šíp V., Ovesná J. (2006): Quantification of Fusarium culmorum in Wheat and Barley Tissues Using Real-Time PCR in Comparison with DON Content. Journal of Phytopathology, 154, 603-611 https://doi.org/10.1111/j.1439-0434.2006.01154.x
 
Li Xin, Shin Sanghyun, Heinen Shane, Dill-Macky Ruth, Berthiller Franz, Nersesian Natalya, Clemente Thomas, McCormick Susan, Muehlbauer Gary J. (2015): Transgenic Wheat Expressing a Barley UDP-Glucosyltransferase Detoxifies Deoxynivalenol and Provides High Levels of Resistance to Fusarium graminearum. Molecular Plant-Microbe Interactions, 28, 1237-1246 https://doi.org/10.1094/MPMI-03-15-0062-R
 
Lysoe E., Klemsdal S. S., Bone K. R., Frandsen R. J. N., Johansen T., Thrane U., Giese H. (2006): The PKS4 Gene of Fusarium graminearum Is Essential for Zearalenone Production. Applied and Environmental Microbiology, 72, 3924-3932 https://doi.org/10.1128/AEM.00963-05
 
MERHEJ JAWAD, URBAN MARTIN, DUFRESNE MARIE, HAMMOND-KOSACK KIM E., RICHARD-FORGET FLORENCE, BARREAU CHRISTIAN (2012): The velvet gene, FgVe1, affects fungal development and positively regulates trichothecene biosynthesis and pathogenicity in Fusarium graminearum. Molecular Plant Pathology, 13, 363-374 https://doi.org/10.1111/j.1364-3703.2011.00755.x
 
Nagl Veronika, Schwartz Heidi, Krska Rudolf, Moll Wulf-Dieter, Knasmüller Siegfried, Ritzmann Mathias, Adam Gerhard, Berthiller Franz (2012): Metabolism of the masked mycotoxin deoxynivalenol-3-glucoside in rats. Toxicology Letters, 213, 367-373 https://doi.org/10.1016/j.toxlet.2012.07.024
 
Nicolaisen Mogens, Supronienė Skaidrė, Nielsen Linda Kærgaard, Lazzaro Irene, Spliid Niels Henrik, Justesen Annemarie Fejer (2009): Real-time PCR for quantification of eleven individual Fusarium species in cereals. Journal of Microbiological Methods, 76, 234-240 https://doi.org/10.1016/j.mimet.2008.10.016
 
Peplow A. W., Tag A. G., Garifullina G. F., Beremand M. N. (2003): Identification of New Genes Positively Regulated by Tri10 and a Regulatory Network for Trichothecene Mycotoxin Production. Applied and Environmental Microbiology, 69, 2731-2736 https://doi.org/10.1128/AEM.69.5.2731-2736.2003
 
Pfaffl M. W. (): A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29, 45e-45 https://doi.org/10.1093/nar/29.9.e45
 
Ponts Nadia, Pinson-Gadais Laetitia, Barreau Christian, Richard-Forget Florence, Ouellet Thérèse (2007): Exogenous H 2 O 2 and catalase treatments interfere with Tri genes expression in liquid cultures of Fusarium graminearum. FEBS Letters, 581, 443-447 https://doi.org/10.1016/j.febslet.2007.01.003
 
Rocha O., Ansari K., Doohan F.M. (2005): Effects of trichothecene mycotoxins on eukaryotic cells: A review. Food Additives & Contaminants, 22: 369–378.
 
Schweiger Wolfgang, Boddu Jayanand, Shin Sanghyun, Poppenberger Brigitte, Berthiller Franz, Lemmens Marc, Muehlbauer Gary J., Adam Gerhard (2010): Validation of a Candidate Deoxynivalenol-Inactivating UDP-Glucosyltransferase from Barley by Heterologous Expression in Yeast. Molecular Plant-Microbe Interactions, 23, 977-986 https://doi.org/10.1094/MPMI-23-7-0977
 
Šíp V., Chrpová J., Leišová L., Sýkorová S., Kučera L., Ovesná J. (2008): Effects of genotype, environment and fungicide treatment on development of Fusarium head blight and accumulation of DON in winter wheat grain. Czech Journal of Genetics and Plant Breeding, 43, 16-31 https://doi.org/10.17221/1905-CJGPB
 
Vančo Bernard, Šliková Svetlana, Šudyova Valéria, Šrobárová Antónia (2007): Response to Fusarium culmorum inoculation in barley. Biologia, 62, - https://doi.org/10.2478/s11756-007-0011-x
 
download PDF

© 2019 Czech Academy of Agricultural Sciences