Electron microscopy of structures present in embryonic cells of plants infected with Plum pox virus
Jaroslav Polák, Milan Jokeš, Miloslava Ducháčová, Alena Hauptmanová, Petr Komínekhttps://doi.org/10.17221/28/2008-PPSCitation:Polák J., Jokeš M., Ducháčová M., Hauptmanová A., Komínek P. (2008): Electron microscopy of structures present in embryonic cells of plants infected with Plum pox virus . Plant Protect. Sci., 44: 81-84.
Electron microscopy was used to detect the presence of virus particles or inclusions in growth tips and parenchymatic cells of leaves of plum, apricot and peach trees artificially infected with Plum pox virus (PPV). Typical pinwheels were found in ultrathin sections of leaves of PPV infected plums, apricots and peaches. Filamentous particles or their aggregates approximately 750 nm long were found in ultrathin sections of growth tips of plum, apricot, and peach shoots with a diameter of 0.5 mm. Pinwheels were never present in embryonic cells. No virus particles were found in ultrathin sections of growth tips of PPV infected plum, apricot and peach shoots of 0.2 mm in diameter. Embryonic cells of growth tips up to 0.2 mm in diameter are PPV free. PPV particles are present in growth tips at a distance 0.2–0.5 mm from the top; the virus is probably multiplied in this part of the growth tips.
sharka; herbaceous and woody hosts; meristematic cells; growth tips; PPV aggregates; ultrathin sections