Control of rice blast disease caused by Magnaporthe oryzae by application of antifungal nanomaterials from Emericella nidulans
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Metabolites of Emericella nidulans (EN) were separated by chromatographic methods from crude hexane included emericellin and sterigmatocystin, while crude ethyl acetate found demethylsterigmatocystin. These metabolites proved to be antagonistic to Magnaporthe oryzae, the causal agent of rice blast. Crude extracts and nano-particles derived from EN inhibited M. oryzae. The ethyl acetate crude extract derived inhibited M. oryzae with an effective dose (ED50) of 66 μg/mL. The nanoparticles showed better inhibition of M. oryzae than crude extracts at low concentrations. Nanoparticles, namely from crude ethyl acetate, crude methanol and crude hexane of EN were active against M. oryzae with ED50 of 4.2 μg/mL, 4.5 μg/mL, 8.9 μg/mL, respectively. It detected sakuranetin (rate of flow value is 0.09) in nano-EN treated rice leaves. These nanoparticles inhibited M. oryzae and acted as a new elicitor to induce immunity.

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