Antifungal activities of Chaetomium spp. against Fusarium wilt of tea
N. Huu Phong, W. Pongnak, K. Soytonghttps://doi.org/10.17221/34/2015-PPSCitation:Huu Phong N., Pongnak W., Soytong K. (2016): Antifungal activities of Chaetomium spp. against Fusarium wilt of tea. Plant Protect. Sci., 52: 10-17.
An isolate of Fusarium denominated as NHP-Fusa-2 from tea wilt and root-rot diseased sample collected in Vietnam was identified as Fusarium oxysporum based on molecular analysis of translation elongation factor-1α sequence. Interestingly, it is the first time F. oxysporum is reported as a causal pathogen of wilt and root-rot disease of tea in Vietnam. Chaetomium spp. were investigated to control F. oxysporum NHP-Fusa-2 in in vitro test. Three antagonists (Ch. cupreum CC3003, Ch. globosum CG05, and Ch. lucknowense CL01) inhibited mycelial growth by 31.69–34.03% and reduced conidial production of the pathogen by 67.25–75.92% in the bi-culture antagonistic test after 30 days with non-significant difference in their effect. All the crude extracts of these antagonists significantly inhibited mycelial growth and conidial production of NHP-Fusa-2. MeOH extract of CC3003 was more effective in conidial inhibition of the pathogen than the others, the effective dose (ED50) was 85.30 μg/ml of which, it was hexane (49.32 μg/ml) and EtOAc extract (62.17 μg/ml) in the case of CG05 and CL01, respectively.Keywords:Chaetomium species; Fusarium oxysporum; growth; sporulation; inhibitionReferences:
Bhagat I., Chakraborty B. (2010): Defense response triggered by Sclerotium rolfsii in tea plant. ECOPRINT, 17: 69–76.Charoenporn C., Kanokmedhakul S., Lin F.C., Poeaim S., Soytong K. (2010): Evaluation of bio-agent formulations to control Fusarium wilt of tomato. African Journal of Biotechnology, 9: 5836–5844.Chen Tzong- Mao (1982): Diseases of Tea and Their Control in the People's Republic of China. Plant Disease, 66, 961- https://doi.org/10.1094/PD-66-961Pietro A. Di (1992): Role of Antibiotics Produced by Chaetomium globosum in Biocontrol of Pythium ultimum, a Causal Agent of Damping-Off. Phytopathology, 82, 131- https://doi.org/10.1094/Phyto-82-131Geiser David M., del Mar Jiménez-Gasco María, Kang Seogchan, Makalowska Izabela, Veeraraghavan Narayanan, Ward Todd J., Zhang Ning, Kuldau Gretchen A., O'donnell Kerry (2004): FUSARIUM-ID v. 1.0: A DNA Sequence Database for Identifying Fusarium. European Journal of Plant Pathology, 110, 473-479 https://doi.org/10.1023/B:EJPP.0000032386.75915.a0Hainsworth E. (1952): Tea Pests and Diseases, and Their Control: With Special Reference to North East India. Cambridge, W. Heffer: 20–39.Kanokmedhakul Somdej, Kanokmedhakul Kwanjai, Nasomjai Pitak, Louangsysouphanh Sysavad, Soytong Kasem, Isobe Minoru, Kongsaeree Palangpon, Prabpai Samran, Suksamrarn Apichart (2006): Antifungal Azaphilones from the Fungus Chaetomiumcupreum CC3003. Journal of Natural Products, 69, 891-895 https://doi.org/10.1021/np060051vLehmann-Danzinger H. (2000): Diseases and pests of tea: Overview and possibilities of integrated pest and disease management. Journal of Agriculture in the Tropics and Subtropics, 101: 13–38.Leslie J.F., Summerell B.A. (2006): The Fusarium Laboratory Manual. Iowa, Blackwell Publishing: 9–10.Liu D., Coloe S., Baird R., Pedersen J. (2000): Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology, 38: 471.Nguyen V.H. (2001): Integrated Pest Management of Green Leafhopper, Thrips, Red Spider Mite and Mosquito Bug on Tea. Vietnam, Hanoi Agicultural Publisher: 104–105 (in Vietnamese).Onsando J. M., Wargo P. M., Waudo S. W. (1997): Distribution, Severity, and Spread of Armillaria Root Disease in Kenya Tea Plantations. Plant Disease, 81, 133-137 https://doi.org/10.1094/PDIS.19188.8.131.52Park Joong-Hyeop, Choi Gyung Ja, Jang Kyoung Soo, Lim He Kyoung, Kim Heung Tae, Cho Kwang Yun, Kim Jin-Cheol (2005): Antifungal activity against plant pathogenic fungi of chaetoviridins isolated from Chaetomium globosum. FEMS Microbiology Letters, 252, 309-313 https://doi.org/10.1016/j.femsle.2005.09.013Sibounnavong P., Sibounnavong P.S., Kanokmedhakul S., Soytong K. (2012): Antifungal activities of Chaetomium brasilense CB01 and Chaetomium cupreum CC03 against Fusarium oxysporum f.sp. lycopersici race 2. Journal of Agricultural Technology, 8: 1029–1038.Soytong K., Kanokmedhakul S., Kukongviriyapa V., Isobe M. (2001): Application of Chaetomium species (Ketomium) as a new broad spectrum biological fungicide for plant disease control: A review article. Fungal Diversity, 7: 1–15.Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. (): MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725-2729 https://doi.org/10.1093/molbev/mst197von Arx J.A., Guarro J., Figueras M.J. (1986): The Ascomycete Genus Chaetomium. Nova Hedwigia Beihefte, Lubrecht & Cramer Ltd.Zeiss M.R., Braber K.D. (2001): Tea IPM Ecological Guide: A Trainer’s Reference Guide on Crop Development, Major Agronomic Practices, and Disease and Insect Management in Small-holders’ Tea Cultivation in Northern Vietnam. CIDSE/Vietnam. Available at http://www.adam-project-vietnam.net/index.php/projets_vi/content/download/7438/45698 /file/Tea%20IPM%20guide.pdfZhang HaiYan, Yang Qian (2007): Expressed sequence tags-based identification of genes in the biocontrol agent Chaetomium cupreum. Applied Microbiology and Biotechnology, 74, 650-658 https://doi.org/10.1007/s00253-006-0701-2Zhang Q., Li H.-Q., Zong S.-C., Gao J.-M., Zhang A.-L. (2012): Chemical and Bioactive Diversities of the Genus Chaetomium Secondary Metabolites. Mini-Reviews in Medicinal Chemistry, 12, 127-148 https://doi.org/10.2174/138955712798995066