Identification of rhizobacteria that increase yield and plant tolerance to angular leaf spot disease in cucumber

https://doi.org/10.17221/41/2017-PPSCitation:Akköprü A., Özaktan H. (2018): Identification of rhizobacteria that increase yield and plant tolerance to angular leaf spot disease in cucumber. Plant Protect. Sci., 54: 67-73.
download PDF

The biological control of angular leaf spot disease (ALS) of cucumbers (Cucumis sativus), caused by Pseudomonas syringae pv. lachrymans (Psl), using promising rhizobacteria (RB) and to compare RB efficacy to that of acibenzolar-S-methyl (ASM) was investigated. Effects of ASM and RB isolate Pseudomonas putida AA11/1 that was isolated from the healthy cucumber root surface on disease severity and plant growth were evaluated using ALS-susceptible and tolerant cucumber cultivars in a growth chamber and a soilless growing system. ASM and AA11/1 significantly reduced average disease severity of ALS by 69 and 34% in the susceptible cultivar and 92 and 21% in the tolerant cultivar, respectively. ASM treatment significantly reduced Psl populations, but AA11/1 did not inhibit Psl growth in either cultivar. In the soilless system, disease severity was limited by either ASM or AA11/1, whereas only AA11/1 treatments significantly increased cucumber yield by 68 and 33% in the susceptible and tolerant cultivar, respectively.

References:
Akköprü A. (2012): Investigations on biological control of angular leaf spot disease of cucumber (Pseudomonas syringae pv. lachrymans) by some rhizobacteria. [Doctoral Thesis.] İzmir, Ege University. (in Turkish)
 
Asghar H.N., Zahir Z.A., Arshad M., Khaliq A. (2002): Relationship between in vitro production of auxins by rhizobacteria and their growth-promoting activities in Brassica juncea L. Biology and Fertility of Soils., 35: 231–237.
 
Bakker Albert W., Schippers Bob (1987): Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas SPP-mediated plant growth-stimulation. Soil Biology and Biochemistry, 19, 451-457 https://doi.org/10.1016/0038-0717(87)90037-X
 
Block A. (2005): Systemic Acquired Tolerance to Virulent Bacterial Pathogens in Tomato. PLANT PHYSIOLOGY, 138, 1481-1490 https://doi.org/10.1104/pp.105.059246
 
Buonaurio R., Scarponi L., Ferrara M., Sidoti P., Bertona A. (2002): Induction of systemic acquired resistance in pepper plants by acibenzolar-S-methyl against bacterial spot disease. European Journal of Plant Pathology, 108: 41–49.https://doi.org/10.1023/A:1013984511233
 
Doss M., Hevisi M. (1981): Systemic acquired resistance of cucumber to Pseudomonas lachrymans as expressed in suppression of symptoms, but not in multiplication of bacteria. Acta Phytopathologica Academiae Scientiarum Hungaricae, 16: 269–272.
 
Durrant W.E., Dong X. (2004): SYSTEMIC ACQUIRED RESISTANCE. Annual Review of Phytopathology, 42, 185-209 https://doi.org/10.1146/annurev.phyto.42.040803.140421
 
Gül A., Tüzel İ.H., Okur B., Tuncay Ö., Aykut N., Engindeniz S. (2000): Cucumber cultivation with soilless growing technique. Tübitak, TAPAR yayınları. (in Turkish)
 
Gül Ayşe, Özaktan Hatice, Kıdoğlu Funda, Tüzel Yüksel (2013): Rhizobacteria promoted yield of cucumber plants grown in perlite under Fusarium wilt stress. Scientia Horticulturae, 153, 22-25 https://doi.org/10.1016/j.scienta.2013.01.004
 
Hammerschmidt R. (2009): Systemic acquired resistance. In: Van Loon L.C. (ed.): Advances in Botanical Research, Plant Innate Immunity. London, Elsevier: 174–222.
 
HUKKANEN ANNE, KOKKO HARRI, BUCHALA ANTONY, HÄYRINEN JUKKA, KÄRENLAMPI SIRPA (2008): Benzothiadiazole affects the leaf proteome in arctic bramble ( Rubus arcticus ). Molecular Plant Pathology, 9, 799-808 https://doi.org/10.1111/j.1364-3703.2008.00502.x
 
Jetiyanon Kanchalee, Kloepper Joseph W (2002): Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biological Control, 24, 285-291 https://doi.org/10.1016/S1049-9644(02)00022-1
 
Khalid A., Arshad M., Zahir Z.A. (2004): Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. Journal of Applied Microbiology, 96, 473-480 https://doi.org/10.1046/j.1365-2672.2003.02161.x
 
Louws F. J., Wilson M., Campbell H. L., Cuppels D. A., Jones J. B., Shoemaker P. B., Sahin F., Miller S. A. (2001): Field Control of Bacterial Spot and Bacterial Speck of Tomato Using a Plant Activator. Plant Disease, 85, 481-488 https://doi.org/10.1094/PDIS.2001.85.5.481
 
Manceau C., Brin C. (2003): Pathovars of Pseudomonas syringae are structured in genetic populations allowing the selection of specific markers for their detection in plant samples. In: Book of Abstracts. 6th International Conference on Pseudomonas syringae pathovars and related pathogens, Sept 15–19, 2003, Maratea, Italy.
 
Mandal B., Mandal S., Csinos A. S., Martinez N., Culbreath A. K., Pappu H. R. (2008): Biological and Molecular Analyses of the Acibenzolar-S-Methyl-Induced Systemic Acquired Resistance in Flue-Cured Tobacco Against Tomato spotted wilt virus. Phytopathology, 98, 196-204 https://doi.org/10.1094/PHYTO-98-2-0196
 
Mecey C., Hauck P., Trapp M., Pumplin N., Plovanich A., Yao J., He S. Y. (2011): A Critical Role of STAYGREEN/Mendel's I Locus in Controlling Disease Symptom Development during Pseudomonas syringae pv tomato Infection of Arabidopsis. PLANT PHYSIOLOGY, 157, 1965-1974 https://doi.org/10.1104/pp.111.181826
 
MEZIANE HAMID, VAN DER SLUIS IENTSE, VAN LOON LEENDERT C., HÖFTE MONICA, BAKKER PETER A. H. M. (2005): Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants. Molecular Plant Pathology, 6, 177-185 https://doi.org/10.1111/j.1364-3703.2005.00276.x
 
Nautiyal C.Shekhar (1999): An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, 170, 265-270 https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
 
Pieterse Corné M.J., Zamioudis Christos, Berendsen Roeland L., Weller David M., Van Wees Saskia C.M., Bakker Peter A.H.M. (2014): Induced Systemic Resistance by Beneficial Microbes. Annual Review of Phytopathology, 52, 347-375 https://doi.org/10.1146/annurev-phyto-082712-102340
 
Romero A. M., Kousik C. S., Ritchie D. F. (2001): Resistance to Bacterial Spot in Bell Pepper Induced by Acibenzolar- S -Methyl. Plant Disease, 85, 189-194 https://doi.org/10.1094/PDIS.2001.85.2.189
 
Saharan B., Nehra V. (2011). Plant growth promoting rhizobacteria: a critical review. Life Sciences and Medicine Research, 2011: 1–30.
 
Scheck Heather J. (1996): Copper and Streptomycin Resistance in Strains of Pseudomonas syringae from Pacific Northwest Nurseries. Plant Disease, 80, 1034- https://doi.org/10.1094/PD-80-1034
 
Schwyn Bernhard, Neilands J.B. (1987): Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160, 47-56 https://doi.org/10.1016/0003-2697(87)90612-9
 
van Loon L. C. (2007): Plant responses to plant growth-promoting rhizobacteria. European Journal of Plant Pathology, 119, 243-254 https://doi.org/10.1007/s10658-007-9165-1
 
WALTERS D. R., FOUNTAINE J. M. (2009): Practical application of induced resistance to plant diseases: an appraisal of effectiveness under field conditions. The Journal of Agricultural Science, 147, 523- https://doi.org/10.1017/S0021859609008806
 
Harayama Shigeaki, Arnold Dawn L., Jackson Robert W., Yamamoto Satoshi, Kasai Hiroaki, Vivian Alan (2000): Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology, 146, 2385-2394 https://doi.org/10.1099/00221287-146-10-2385
 
YANO Hiroshi, FUJII Hiroshi, MUKOO Hideo, SHIMURA Masaru, WATANABE Tetsuro, SEKIZAWA Yasuharu (1978): On the enzymic inactivation of dihydrostreptomycin by Pseudomonas lachrymans, cucumber angular leaf spot bacterium: Isolation and structural resolution of the inactivated product.. Japanese Journal of Phytopathology, 44, 413-419 https://doi.org/10.3186/jjphytopath.44.413
 
download PDF

© 2019 Czech Academy of Agricultural Sciences