Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast
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The Magnaporthe oryzae isolate PO2 was proven to cause rice blast var. PSL 2 in this study. Chaetomium elatum, Chaetomium lucknowense and Chaetomium brasiliense were antagonised to M. oryzae.  The hexane extract of C. brasiliense gave the highest inhibition of the spore production with an ED50 of 35 ppm, the EtOAC extract of C. lucknowense inhibited the spore production at 57 ppm and the EtOAC extracts of C. elatum inhibited the spore production at 106 ppm. The nano-CLM (C. lucknowense) inhibited the spore production at 5.24 ppm, the nano-CBH (C. brasiliense) inhibited the spore production at 6.86 ppm and the nano-CEE (C. elatum) inhibited the spore production at 7.89 ppm. The rice leaves treated with nano-CBH from C. brasiliense produced Sakuranertin and Oryzalexin B as seen on the thin layer chromatography where the Rf value was 0.08 assumed to be Sakuranertin, and the Rf value of 0.28 supposed to be Oryzalexin B. It was found that the nanoparticles act as elicitors to induce immunity in rice plants through the production of phytoalexin.

Akatsuka T., Kodama O., Kato H., Kono Y., Takeuchi S. (1983): 3-Hydroxy-7-oxo-sandaraco- pimaradiene (Oryzalexin A), a new phytoalexin isolated from rice blast leaves. Agricultural and Biological Chemistry, 77: 1141–1148.
Altschul S.F., Madden T.L., Schaffer A.A., Zhang J.H., Zhang Z., Miller W., Lipman D.J. (1997): Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25: 3389–3402.
Dar J., Soytong K. (2014): Construction and characterization of copolymer nanomaterials loaded with bioactive compounds from Chaetomium species. Journal of Agricultural Technology, 10: 823–831.
Devi S.J.S.R., Singh K., Umakanth B., Vishalakshi B., Renuka P., Sudhakar K.V., Prasad M. S., Viraktamath B.C., Babu V.R., Madhav M.S. (2015): Development and identification of novel rice blast resistant sources and their characterization using molecular markers. Rice Science, 22: 300–308.
Dillon V.M., Overton J., Grayer R.J., Harbone J. (1997): Differences in phytoalexin response among rice cultivars of different resistance to blast. Phytochemistry, 44: 559–603.
Elibol O.H., Morisette D.D., Denton J.P., Bashir R. (2003): Integrated nanoscale silicon sensors using top-down fabrication. Applied Physics Letters, 83: 4613–4615.
FAO (2009): FAO’s director-general on how to feed the world in 2050. Insights from an Expert Meeting at FAO, 1: 1–35.
Hasegawa M., Mitsuhara I., Seo S., Okada K., Yamane H., Iwai T., Ohashi Y. (2014): Analysis on blast fungus-responsive characters of a flavonoid phytoalexin sakuranetin; Accumulation in infected rice leaves, antifungal activity and detoxification by fungus. Molecules, 19: 11404–11418.
Jagadeesh D., Prasanna Kumar M.K., Chandrakanth R., Devaki N.S. (2018): Molecular diversity of internal transcribed spacer among the monoconidial isolates of Magnaporthe oryzae isolated from rice in Southern Karnataka, India. Journal of Genetic Engineering and Biotechnology, 16: 631–638.
Khumkomkhet P., Kanokmedhakul S., Kanokmedhakul K., Hahnvajanawong C., Soytong K. (2009): Antimalarial and cytotoxic depsidones from the fungus Chaetomium brasiliense. Journal of Natural Products, 72: 1 487–1491.
Kodama O., Miyakawa J., Akatsuka T., Kiyosawa S. (1992): Sakuranetin, a flavanone phytoalexin from ultraviolet-irradiated rice leaves. Phytochemistry, 31: 3807–3809.
Li M., Huang Q., Wu Y. (2011): A novel chitosan-poly (lactide) copolymer and its submicron particles as imidacloprid carriers. Pest Management Science, 67: 831–836.
Miah G., Rafill M.Y., Ismail M.R., Puteh A.B., Rahim A.B., Asfaliza R., Latif M.A. (2013): Blast resistance in rice:
a review of conventional breeding to molecular approaches. Molecular Biology Reports, 40: 2369–2388.
Ou S.H. (1985): Rice Diseases. UK, Common Wealth Mycological Institute.
Pornsuriya C., Soytong K., Poeaim S., Kanokmedhakul S., Khumkomkhet P., Lin F.C., Wang H.K., Hyde K.D. (2011): Chaetomium siamense sp. nov., a soil isolate from Thailand, produces a new chaetoviridin, G. Mycotaxon, 115: 17–19.
Rai M., Ingle A. (2012): Role of nanotechnology in agriculture with special reference to management of insect-pest. Applied Microbiology and Biotechnology, 94: 287–293.
Sibounnavong P., Kanokmedhakul S., Soytong K. (2011): Antifungal metabolites from antagonistic fungi used to control tomato wilt fungus Fusarium oxysporum f. sp. lycopersici. African Journal of Biotechnology, 10: 19714–19722.
Song J.J., Soytong K. (2016): Antifungal activity of Chaetomium elatum against Pyricularia oryze causing rice blast. International Journal of Agricultural Technology, 12: 1437–1447.
Song J.J., Soytong K. (2016): Antifungal activity of Chaetomium elatum against Pyricularia oryze causing rice blast. Journal of Agricultural Technology, 12: 1437–1447.
Song J.J., Soytong K., Kanokmedhakul S. (2017): Fungal metabolites of Chaetomium lucknowense for inhibition of a rice blast pathogen, Pyricularia oryzae. International Journal of Agricultural Technology, 13: 1621–1626.
Soutter W. (2013): Nanotechnology in agriculture. Available at ID=3141#1%204 (accessed May 25, 2013).
Soytong K. (1992): Antagonism of Chaetomium cupreum to Pyricularia oryzae. Journal of Plant Protection in the Tropics, 9: 17–23.
Soytong K. (2014): Bio-formulation of Chaetomium cochliodes for controlling brown leaf spot of rice. Journal of Agricultural Technology, 10: 321–337.
Soytong K., Charoenporn C., Kanokmedhakul S. (2013): Evaluation of microbial elicitors to induce plant immunity for tomato wilt. African Journal of Microbiology Research, 7: 1993–2000.
Soytong K., Kanokmedhakul S., Kukongviriyapan V., Isobe M. (2001): Application of Chaetomium species (Ketomium) as a new broad spectrum biological fungicide for plant disease control: a review article. Fungal Diversity, 7: 1–15.
Tamura K., Dudley J., Nei M., Kumar S. (2007): MEGA4: Molecular evolutionary genetics analysis (MEGA) software
version 4.0. Molecular Biology and Evolution, 24: 1596–1599.
Tann H., Soytong K. (2017): Bioformulations and nanoproduct from Chaetomium cupreum CC3003 to control leaf spot of rice var. Sen Pidoa in Cambodia. International Journal of Plant Biology, 7: 59–63.
Tann Y., Soytong K. (2016): Effects of nanoparticles loaded with Chaetomium globosum KMITL0805 extracts against leaf spot of rice var Sen Pidoa. Malaysian Applied Biology, 45: 1–7.
Thohinung S., Kanokmedhakul S., Kanokmedhakul K., Kukongviriyapan V., Tusskorn O., Soytong K. (2010): Cytotoxic 10-(indol-3-yl)-[13]cytochalasans from the fungus Chaetomium elatum ChE01. Archives of Pharmacal Research. 33: 1135–1141.
Uehara K. (1958): On the production of phytoalexin by the host plant as a result of interaction between the rice plant and the blast fungus (Pyricularia oryzae Cav.). Annals of the Phytopathological Society of Japan, 23: 127–130.
Vilavong S., Soytong K. (2017): Application of a new bio-formulation of Chaetomium cupreum for biocontrol of Colletotrichum gloeosporioides causing coffee anthracnose on arabica variety in Laos. AGRIVITA, Journal of Agricultural Science, 39: 303–310.
Yew S.M., Chan C.L., Lee K.W., Na S.L., Tan R., Hoh C.C., Yee W.Y., Ngeow Y.F., Ng K.P. (2014): A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. PLoS ONE 9(8): e104352.
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