Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants

https://doi.org/10.17221/42/2018-PPSCitation:Cejnar P., Ohnoutková L., Ripl J., Kundu J.K. (2019): Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants. Plant Protect. Sci., 55: 81-89.
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We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus. Infected plants were generated using both infectious clones, whereas the agroinoculation method was able to produce strong systemic infection in all three tested cultivars of wheat and Triticum monococcum, comparable to plants inoculated by viruliferous P. alienus. Infection was confirmed by DAS-ELISA, and WDV titres were quantified using qPCR. The levels of remaining bacterial plasmid DNA were also confirmed to be zero.

References:
Bendahmane M. (1996): Identification and Characterization of Wheat Dwarf Virus from France Using a Rapid Method for Geminivirus DNA Preparation. Phytopathology, 85, 1449- https://doi.org/10.1094/Phyto-85-1449
 
Benkovics A. H., Vida G., Nelson D., Veisz O., Bedford I., Silhavy D., Boulton M. I. (2010): Partial resistance to Wheat dwarf virus in winter wheat cultivars. Plant Pathology, 59, 1144-1151 https://doi.org/10.1111/j.1365-3059.2010.02318.x
 
Boulton M.I. (2008): Construction of infectious clones for DNA viruses: mastreviruses. Methods in Molecular Biology, 451: 503–523.
 
Boulton Margaret I., Buchholz Wallace G., Marks Melanie S., Markham Peter G., Davies Jeffrey W. (1989): Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. Plant Molecular Biology, 12, 31-40 https://doi.org/10.1007/BF00017445
 
Fang Chen Dong, Dale Philip J. (1992): A comparison of methods for delivering DNA to wheat: the application of wheat dwarf virus DNA to seeds with exposed apical meristems. Transgenic Research, 1, 93-100 https://doi.org/10.1007/BF02513026
 
Czosnek H., Kheyr-Pour A., Gronenborn B., Remetz E., Zeidan M., Altman A., Rabinowitch H. D., Vidavsky S., Kedar N., Gafni Y., Zamir D. (1993): Replication of tomato yellow leaf curl virus (TYLCV) DNA in agroinoculated leaf discs from selected tomato genotypes. Plant Molecular Biology, 22, 995-1005 https://doi.org/10.1007/BF00028972
 
Dale P.J., Marks M.S., Brown M.M., Woolston C.J., Gunn H.V., Mullineaux P.M., Lewis D.M., Kemp J.M., Chen D.F., Gilmour D.M., Flavell R.B. (1989): Agroinfection of wheat: Inoculation of in vitro grown seedlings and embryos. Plant Science, 63, 237-245 https://doi.org/10.1016/0168-9452(89)90249-5
 
Dekker Elise L., Woolston Crispin J., Xue Yongbiao, Cox Brian, Mullineaux Philip M. (1991): Transcript mapping reveals different expression strategies for the bicistronic RNAs of the geminivirus wheat dwarf virus. Nucleic Acids Research, 19, 4075-4081 https://doi.org/10.1093/nar/19.15.4075
 
Dinant Sylvie, Ripoll Christophe, Pieper Mark, David Chantal (2004): Phloem specific expression driven by wheat dwarf geminivirus V-sense promoter in transgenic dicotyledonous species. Physiologia Plantarum, 121, 108-116 https://doi.org/10.1111/j.0031-9317.2004.00296.x
 
Grimsley N., Hohn B., Hohn T., Walden R. (1986): "Agroinfection," an alternative route for viral infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences, 83, 3282-3286 https://doi.org/10.1073/pnas.83.10.3282
 
Grimsley Nigel, Hohn Thomas, Davies Jeffrey W., Hohn Barbara (1987): Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature, 325, 177-179 https://doi.org/10.1038/325177a0
 
Grimsley Nigel H., Ramos Cynthia, Hein Thomas, Hohn Barbara (1988): Meristematic Tissues of Maize Plants Are Most Susceptible to Agroinfection With Maize Streak Virus. Nature Biotechnology, 6, 185-189 https://doi.org/10.1038/nbt0288-185
 
Habekuß A., Riedel C., Schliephake E., Ordon F. (2009): Breeding for resistance to insect-transmitted viruses in barley – an emerging challenge due to global warming. Journal für Kulturpflanzen, 61: 53–61.
 
Hayes R. J., Macdonald H., Coutts R. H. A., Buck K. W. (1988): Agroinfection of Triticum aestivum with Cloned DNA of Wheat Dwarf Virus. Journal of General Virology, 69, 891-896 https://doi.org/10.1099/0022-1317-69-4-891
 
Helloco-Kervarrec Christine, Riault Gérard, Jacquot Emmanuel (2002): Biolistic-mediated inoculation of immature wheat embryos with Barley yellow dwarf virus-PAV. Journal of Virological Methods, 102, 161-166 https://doi.org/10.1016/S0166-0934(01)00446-3
 
Himmelbach A., Zierold U., Hensel G., Riechen J., Douchkov D., Schweizer P., Kumlehn J. (2007): A Set of Modular Binary Vectors for Transformation of Cereals. PLANT PHYSIOLOGY, 145, 1192-1200 https://doi.org/10.1104/pp.107.111575
 
Jones H.D., Shewry P.R. (eds) (2009): Transgenic Wheat, Barley and Oats. New York, Humana Press.
 
Kheyr-Pour A., Gronenborn B., Czosner H. (1994): Agroinoculation of Tomato Yellow Leaf Curl Virus (TYLCV) Overcomes the Virus Resistance of Wild Lycopersicon Species. Plant Breeding, 112, 228-233 https://doi.org/10.1111/j.1439-0523.1994.tb00675.x
 
Kis András, Tholt Gergely, Ivanics Milán, Várallyay Éva, Jenes Barnabás, Havelda Zoltán (2016): Polycistronic artificial miRNA-mediated resistance to Wheat dwarf virus in barley is highly efficient at low temperature. Molecular Plant Pathology, 17, 427-437 https://doi.org/10.1111/mpp.12291
 
Kundu J. K., Gadiou S., Červená G. (2009): Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic. Virus Genes, 38, 468-474 https://doi.org/10.1007/s11262-009-0352-3
 
Lapidot Moshe, Weil Galit, Cohen Lidya, Segev Limor, Gaba Victor (2007): Biolistic inoculation of plants with Tomato yellow leaf curl virus DNA. Journal of Virological Methods, 144, 143-148 https://doi.org/10.1016/j.jviromet.2007.04.011
 
Lindblad M., Waern P. (2002): Correlation of wheat dwarf incidence to winter wheat cultivation practices. Agriculture Ecosystems & Environment, 92: 115–122.
 
Lindsten K., Lindsten B., Abdelmoeti M., Junti N. (1980): Purification and some properties of Wheat dwarf virus. In: Proceeding 3rd Conference on Virus Diseases of Gramineae in Europe. Rothamsted, UK: 27–31.
 
Lindsten K., Vacke J. (1991): A possible barley adapted strain of wheat dwarf virus (WDV). Acta Phytopathologica et Entomologica Hungarica, 26: 175–180.
 
Liu Yan, Jin Wen, Wang Liang, Wang Xifeng (2014): Replication-associated proteins encoded by Wheat dwarf virus act as RNA silencing suppressors. Virus Research, 190, 34-39 https://doi.org/10.1016/j.virusres.2014.06.014
 
Marks M.S., Kemp J.M., Woolston C.J., Dale P.J. (1989): Agroinfection of wheat: A comparison of Agrobacterium strains. Plant Science, 63, 247-256 https://doi.org/10.1016/0168-9452(89)90250-1
 
Martin D. P., Rybicki E. P. (2000): Improved Efficiency of Zea mays Agroinoculation with Maize streak virus. Plant Disease, 84, 1096-1098 https://doi.org/10.1094/PDIS.2000.84.10.1096
 
Ramsell J. N. E., Boulton M. I., Martin D. P., Valkonen J. P. T., Kvarnheden A. (2009): Studies on the host range of the barley strain of Wheat dwarf virus using an agroinfectious viral clone. Plant Pathology, 58, 1161-1169 https://doi.org/10.1111/j.1365-3059.2009.02146.x
 
Redinbaugh M.G (2003): Transmission of Maize streak virus by vascular puncture inoculation with unit-length genomic DNA. Journal of Virological Methods, 109, 95-98 https://doi.org/10.1016/S0166-0934(03)00044-2
 
Ripl J., Kundu J.K. (2015): Cynosurus cristatus, a new host of Wheat dwarf virus in the Czech Republic. Journal of Plant Pathology, 97: 547–547.
 
Schubert Jörg, Habekuß Antje, Kazmaier Katja, Jeske Holger (2007): Surveying cereal-infecting geminiviruses in Germany—Diagnostics and direct sequencing using rolling circle amplification. Virus Research, 127, 61-70 https://doi.org/10.1016/j.virusres.2007.03.018
 
Širlová L., Vacke J., Chaloupková M. (2010): Reaction of selected winter wheat varieties to autumnal infection with Wheat dwarf virus. Plant Protection Science, 41, 1-7 https://doi.org/10.17221/2732-PPS
 
Tholt G., Kis A., Medzihradszky A., Szita É., Tóth Z., Havelda Z., Samu F. (2018): Could vectors’ fear of predators reduce the spread of plant diseases?. Scientific Reports, 8, - https://doi.org/10.1038/s41598-018-27103-y
 
Tobias I., Shevchenko O., Kiss B., Bysov A., Snihur H., Polischuk V., Palkovics L. (2011): Comparison of the nucleotide sequences of Wheat dwarf virus (WDV) isolates from Hungary and Ukraine. Polish Journal of Microbiology, 60: 125–131.
 
Vacke Josef (1961): Wheat dwarf virus disease. Biologia Plantarum, 3, 228-233 https://doi.org/10.1007/BF02933566
 
Vacke J. (1972): Host plants range and symptoms of wheat dwarf virus. Vědecké práce Výzkumného ústavu rostlinné výroby, Praha-Ruzyně, 17: 151–162.
 
Vacke J Cibulka R. (1999): Silky bent grass (Apera spica-venti [L.] Beauv.) – a new host and reservoir of wheat dwarf virus. Plant Protection Science, 35, 47-50 https://doi.org/10.17221/9674-PPS
 
Vacke J., Cibulka R. (2000): Comparison of DAS-ELISA and enzyme amplified ELISA for detection of Wheat dwarf virus in host plants and leafhopper vectors. Plant Protection Science, 36, 41-45 https://doi.org/10.17221/9620-PPS
 
Woolston Crispin J., Barker Richard, Gunn Helen, Boulton Margaret I., Mullineaux Philip M. (1988): Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA. Plant Molecular Biology, 11, 35-43 https://doi.org/10.1007/BF00016012
 
ZADOKS J. C., CHANG T. T., KONZAK C. F. (1974): A decimal code for the growth stages of cereals. Weed Research, 14, 415-421 https://doi.org/10.1111/j.1365-3180.1974.tb01084.x
 
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