Using leaf chlorophyll fluorescence for in-season diagnosing herbicide resistance in Echinochloa species at reproductive growth stage C., Kim D. (2018): Using leaf chlorophyll fluorescence for in-season diagnosing herbicide resistance in Echinochloa species at reproductive growth stage. Plant Protect. Sci., 54: 194-202.
download PDF

The chlorophyll fluorescence measurement to diagnose herbicide resistant Echinochloa species at the reproductive (late) growth stage was applied. The significant correlation between Fv/Fm (chlorophyll fluorescence measurements) and fresh weight (whole plant test) and the statistical similarity of R/S ratios between the two tests demonstrated that the chlorophyll fluorescence test could be reliably used to diagnose herbicide resistant Echinochloa spp. at the reproductive growth stage in a shorter period of time (within 10 days) compared with the conventional whole plant test.

Abbaspoor Majid, Streibig Jens C. (2005): Clodinafop changes the chlorophyll fluorescence induction curve. Weed Science, 53, 1-9
ABBASPOOR M, TEICHER HB, STREIBIG JC (2006): The effect of root-absorbed PSII inhibitors on Kautsky curve parameters in sugar beet. Weed Research, 46, 226-235
Ahrens W.H., Arntzen C.J., Stoller E.W. (1981): Chlorophyll fluorescence assay for the determination of triazine resistance. Weed Science, 29: 316–322.
Azmi M., Mortimer M. (2000): Weed species shift in response to serial herbicide application in wet-seeded rice in Malaysia. In: Pandey S. (ed.): Direct Seeding Research Strategies and Opportunities. Philippines, International Rice Research Institute: 357–367.
Beckie Hugh J., Friesen Lyle F., Nawolsky Ken M., Morrison Ian N. (1990): A Rapid Bioassay to Detect Trifluralin-Resistant Green Foxtail (Setaria viridis). Weed Technology, 4, 505-508
BOUTSALIS PETER (2001): Syngenta Quick-Test: A Rapid Whole-Plant Test for Herbicide Resistance 1. Weed Technology, 15, 257-263[0257:SQTARW]2.0.CO;2
Burton James D., Gronwald John W., Somers David A., Gengenbach Burle G., Wyse Donald L. (1989): Inhibition of corn acetyl-CoA carboxylase by cyclohexanedione and aryloxyphenoxypropionate herbicides. Pesticide Biochemistry and Physiology, 34, 76-85
Dayan Franck E., Zaccaro Maria Leticia de M. (2012): Chlorophyll fluorescence as a marker for herbicide mechanisms of action. Pesticide Biochemistry and Physiology, 102, 189-197
Elahifard E., Ghanbari A., Mohassel M.H.R., Zand E., Mirshamsi A., Abbaspoor M. (2013): Measuring chlorophyll fluorescence parameters for rapid detection of ametryn resistant junglerice [Echinochloa colona (L.) Link.]. Plant Knowledge Journal, 2: 76–82.
Fischer Albert J., Ateh Comfort M., Bayer David E., Hill James E. (2000): Herbicide-resistant Echinochloa oryzoides and E. phyllopogon in California Oryza sativa fields. Weed Science, 48, 225-230[0225:HREOAE]2.0.CO;2
Focke M., Lichtenthaler H.K. (1987): Inhibition of the Acetyl-CoA carboxylase of barley chloroplasts by cycloxydim and sethoxydim. Zeitschrift für Naturforschung, 42c: 1361–1363.
Heap I. (2017): The international survey of herbicide resistant weeds. Available at (accessed April 15, 2017).
Dan Hess F. (2000): Light-dependent herbicides: an overview. Weed Science, 48, 160-170[0160:LDHAO]2.0.CO;2
HRAC (2016): Confirming resistance. Available at (accessed Dec 15, 2016).
Im S.H., Park M.W., Yook M.J., Kim D.S. (2009): Resistance to ACCase inhibitor cyhalofop-butyl in Echinochloa crus-galli var. crus-galli collected in Seosan, Korea. Korean Journal of Weed Science, 29: 178–184.
Kaiser Y I, Menegat A, Gerhards R, Kudsk Per (2013): Chlorophyll fluorescence imaging: a new method for rapid detection of herbicide resistance in Alopecurus myosuroides. Weed Research, 53, 399-406
KAUNDUN S S, HUTCHINGS S-J, DALE R P, BAILLY G C, GLANFIELD P (2011): Syngenta ‘RISQ’ test: a novel in-season method for detecting resistance to post-emergence ACCase and ALS inhibitor herbicides in grass weeds. Weed Research, 51, 284-293
Kaundun Shiv S., Hutchings Sarah-Jane, Harris Suzanne C., Jackson Lucy V., Shashi-Kiran Rekha, Dale Richard P., McIndoe Eddie (2014): A Simple In-Season Bioassay for Detecting Glyphosate Resistance in Grass and Broadleaf Weeds Prior to Herbicide Application in the Field. Weed Science, 62, 597-607
Kim J.W. (2016): Geographical distribution and mechanism of ALS inhibitor herbicide resistant E. chinochloa spp. in Korea. [PhD Thesis.] Seoul, Seoul National University.
Kim Do-Soon, Caseley John C., Brain Philip, Riches Charles R., Valverde Bernal E. (2000): Rapid detection of propanil and fenoxaprop resistance in Echinochloa colona. Weed Science, 48, 695-700[0695:RDOPAF]2.0.CO;2
Knezevic Stevan Z., Streibig Jens C., Ritz Christian (2007): Utilizing R Software Package for Dose-Response Studies: The Concept and Data Analysis. Weed Technology, 21, 840-848
LaRossa R.A., Schloss J.V. (1984): The sulfonylurea herbicide sulfometuron methyl is an extremely potent and selective inhibitor of acetolactate synthase in Salmonella typhimurium. Journal of Biological Chemistry, 259: 8753–8757.
Moon Byeong Chul, Cho Seung Hyun, Kwon Oh Do, Lee Sun Gye, Lee Byun Woo, Kim Do Soon (2010): Modelling rice competition with Echinochloa crus-galli and Eleocharis kuroguwai in transplanted rice cultivation. Journal of Crop Science and Biotechnology, 13, 121-126
Norsworthy J.K., Talbert R.E., Hoagland R.E. (1998): Chlorophyll fluorescence for rapid detection of propanil-resistant barnyardgrass (Echinochloa crus-galli). Weed Science, 46: 163–169.
R Development Core Team (2011): R: A language and Environment for Statistical Computing. Vienna, R Foundation for Statistical Computing.
SØBYE K W, STREIBIG J C, CEDERGREEN N (2011): Prediction of joint herbicide action by biomass and chlorophyll a fluorescence. Weed Research, 51, 23-32
Song J.S., Im S.H., Kang S.W., Yook M.J., Kim D.S. (2011): Acetolactate synthase (ALS) inhibitor resistant Echinochloa species evolved in Korea and its mechanism. In: Proceedings Asian Pacific Weed Science Society Conference, Sept 26–29, 2011, Cairns, Queensland, Australia, Vol. 2: 149–155.
Streibig J.C. (1980): Models for curve-fitting herbicide dose response data. Acta Agriculturae Scandinavica, Section B – Soil & Plant Science, 30: 59–63.
STREIBIG J. C., WALKER A., BLAIR A. M., ANDERSON-TAYLOR G., EAGLE D. J., FRIEDLÄNDER H., HACKER E., IWANZIK W., KUDSK P., LABHART C., LUSCOMBE B. M., MADAFIGLIO G., NEL P. C., PESTEMER W., RAHMAN A., RETZLAFF G., ROLA J., STEFANOVIC L., STRAATHOF H. J. M., THIES E. P. (1995): Variability of bioassays with metsulfuron-methyl in soil. Weed Research, 35, 215-224
Tal A., Kotoula-Syka E., Rubin B. (2000): Seed-bioassay to detect grass weeds resistant to acetyl coenzyme A carboxylase inhibiting herbicides. Crop Protection, 19, 467-472
Talbert Ronald E., Burgos Nilda R. (2007): History and Management of Herbicide-resistant Barnyardgrass (Echinochloa Crus-galli) in Arkansas Rice. Weed Technology, 21, 324-331
OORSCHOT J. L. P., LEEUWEN P. H. (1992): Use of fluorescence induction to diagnose resistance of Alopecurus myosuroides Huds. (black-grass) to chlorotoluron. Weed Research, 32, 473-482
Wang Pei, Peteinatos Gerassimos, Li Hui, Gerhards Roland (2016): Rapid in-season detection of herbicide resistant Alopecurus myosuroides using a mobile fluorescence imaging sensor. Crop Protection, 89, 170-177
Zhang C.J., Kim D.S. (2017): Diagnosing ACCase inhibitor – cyhalofop-butyl resistance in Echinochloa crus-galli at various growth stages. Canadian Journal of Plant Science, 97: 286–297.
Zhang C. J., Lim S. H., Kim J. W., Song J. S., Yook M. J., Nah G., Valverde B. E., Kim D. S. (2015): Quantifying herbicide dose–response and resistance in Echinochloa spp. by measuring root length in growth pouches. Canadian Journal of Plant Science, 95, 1181-1192
Zhang C J, Lim S H, Kim J W, Nah G, Fischer A, Kim D S, Kudsk Per (2016): Leaf chlorophyll fluorescence discriminates herbicide resistance in Echinochloa species. Weed Research, 56, 424-433
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti