Susceptibility of field and laboratory strains of Cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) to spinosad pesticide under laboratory conditions M.A.I., Temerak S.A.H., Abdel-Galil F.A.-., Manna S.H.M. (2016): Susceptibility of field and laboratory strains of Cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) to spinosad pesticide under laboratory conditions. Plant Protect. Sci., 52: 128-133.
download PDF
The susceptibility of field and laboratory strains against all instars larvae of S. littoralis to spinosad pesticide after a 24- and 48-h exposure and under laboratory conditions was investigated. As a result against 1st instar larvae, the LC50 values after 24 h were 12 and 0.275 µg/ml for laboratory and field strain, respectively. In addition, the resistance ratio (RR) of 1st instar was 43.64-fold. In this interim, the 48 h LC50 values were 8.7 and 0.18 µg/ml for laboratory and field strain, respectively and the RR was 48.33-fold, which revealed the field strain was more susceptible to spinosad than the laboratory strain. Distinctly similar trend was shown for later instar larvae stages. For instance, in 6th instar larvae, the LC50 values after a 24-h exposure to spinosad were 1100 and 105 µg/ml for the laboratory and field strain, respectively, and the RR value was 10.48-fold. Furthermore, after a 48-h exposure, the LC50 values for laboratory and field strains were 500 and 42 µg/ml, respectively, with RR value being 11.90-fold. On the other hand, according to relative tolerance values, the 6th instar larvae were the most tolerant instar of all the instars tested. The susceptibility of 6th, 5th, and 4th instar larvae was comparable and significantly lower than that of 3rd, 2nd, and 1st instar larvae. However, the 1st instar was the least tolerant. The results implied that spinosad may play a potential role in the control of S. littoralis and, therefore, it is considered a promising tool in integrated pest management program to control Cotton leafworm which is becoming resistant to conventional pesticides in Egypt.
Abbott W. S. (1925): A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology, 18, 265-267
Abo El-Ghar G.E.S., Radwan H.S.A., El-Bermawy Z.A., Zidan L.T.M. (1994): Histopatholgical effects of abamectin, thuringiensin and diflubenzuron on the midgut of Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. Bulletin of the Entomological Society of Egypt, 21: 41–52.
Ahmed M.A.I. (2014): Evaluation of novel neonicotinoid pesticides against cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) under laboratory conditions. Advances in Environmental Biology, 8: 1002–1007.
Ahmed M.A.I., Abdel-Galil F.A., Temerak S.A.S., Manna S.H.M. (2015a): Bio-residual activity of selected biopesticides in comparison with the conventional insecticide Dursban against Cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). American-Eurasian Journal of Sustainable Agriculture, 9 (1): 9–13.
Ahmed M.A.I., Temerak S.A.S., Abdel-Galil F.A., Manna S.H.M. (2015b): Effect of selected host plants on the efficacy of spinosad pesticide on Cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) under laboratory conditions. Advances in Environmental Biology, 9: 372–375.
Arora R.K. (2003): Baseline susceptibility and quantification of resistance in Plutella xylostella (L.) to spinosad. Resistant of Pest Management Newsletter, 12 (2): 27–30.
Aydin H., Gürkan M.O. (2006): The efficacy of spinosad on different strains of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Turkish Journal of Biology, 30: 5–9.
El-Geddawy A.M.H., Ahmed M.A.I., Mohamed S.H. (2014): Toxicological evaluation of selected biopesticides and one essential oil in comparison with Indoxacarb pesticide on cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) under laboratory conditions. American-Eurasian Journal of Sustainable Agriculture, 8 (2): 58–64.
El-Sheikh T.A.A. (2012): Biological, biochemical and histological effects of spinosad, Bacillus thuringiensis var. kurstaki and cypermethrin on the Cotton leafworm, Spodoptera littoralis (Boisd.). Egyptian Academic Journal of Biological Sciences, 4: 113–124.
Funderburk J., Higley L., Buntin G.D. (1993): Concepts and directions in arthropod pest management. Advances in Agronomy, 51: 126–172.
Hendrix W.H., Huckaba R., Nead B., Peterson L., Porteous D., Thompson G. (1997): Tracer insect control. In: Proceedings Beltwide Cotton Conferences, Jan 6–10, 1997, New Orleans, USA, Vol. 2: 1086–1087.
Litchfield J.J., Wilcoxon F. (1949): A simplified method of evaluation dose-effect experiments. Journal of Pharmacology and Experimental Therapeutics, 96: 99–113.
Mascarenhas R. N., Boethel D. J. (1997): Responses of Field-Collected Strains of Soybean Looper (Lepidoptera: Noctuidae) to Selected Insecticides Using an Artificial Diet Overlay Bioassay. Journal of Economic Entomology, 90, 1117-1124
Mascarenhas V. J., Graves J. B., Leonard B. R., Burris E. (1998): Susceptibility of Field Populations of Beet Armyworm (Lepidoptera: Noctuidae) to Commercial and Experimental Insecticides. Journal of Economic Entomology, 91, 827-833
Mesbah H.A., Saad A.S., Mourad A.K., Taman F.A., Mohamed I. (2007): Biological performance of quercetin on the Cotton leafworm larvae, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) and prevailing natural enemies in the Egyptian cotton fields. Communications in Agricultural and Applied Biological Sciences, 72: 611–622.
Moulton J.K., Pepper D.A., Dennehy T.J., Dugger P., Richter D. (1999): Studies of resistance of beet armyworm (Spodoptera exigua) to spinosad in field populations from the southern USA and Southeast Asia. In: Proceedings Beltwide Cotton Conferences, Jan 3–7, 1999, Orlando, Florida, USA. Vol. 2: 884–887.
Liu Nannan, Yue Xin (2000): Insecticide Resistance and Cross-Resistance in the House Fly (Diptera: Muscidae). Journal of Economic Entomology, 93, 1269-1275
Pedigo L.P. (1996): Entomology and Pest Management. 2nd Ed. Englewood Cliffs, Prentice-Hall.
Pineda Samuel, Schneider Marcela-Inés, Smagghe Guy, Martínez Ana-Mabel, Del Estal Pedro, Viñuela Elisa, Valle Javier, Budia Flor (2007): Lethal and Sublethal Effects of Methoxyfenozide and Spinosad on <I>Spodoptera littoralis</I> (Lepidoptera: Noctuidae). Journal of Economic Entomology, 100, 773-780[773:LASEOM]2.0.CO;2
Redding K.D., Nead B.A. (1998): Improving the deposition and coverage of tracer applied to cotton. Down to Earth, 53 (1): 18–21.
Saunders D.G., Bret B.L. (1997): Fate of spinosad in the environment. Down to Earth, 52 (1): 14–20.
Swaroop S., Gilory A.B., Umeura K. (1966): Susceptibility Tests. Statistical Methods in Malaria Eradication. WHO Monograph Seris, No. 5. Geneva, WHO: 118–129.
Temerak S.A. (2002): Historical records of cotton leafworm (Spodoptera littoralis) resistance to conventional insecticides in the field as influenced by the resistance programs in Egypt from 1950–2002. Resistant Pest Management Newsletter, 12 (1): 7–10.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti