Study on the antifungal effect and mycolytic activity of the biocontrol agent Chaetomium subaffine LB-1

Caiyun L., Zhao J., Cao H. (2021): Study on the antifungal effect and mycolytic activity of the biocontrol agent Chaetomium subaffine LB-1. Plant Protect. Sci., 57: 289–296.

download PDF

The antifungal effect and mucolytic activity of a newly screened biocontrol strain Chaetomium subaffine LB-1 were researched in this study. The results found that LB-1 has good antifungal effects on the test plant pathogenic fungi Botrytis cinerea Pers. ex Fr., Fusarium oxysporum f. sp. cucumerinum Owen and Alternaria solani (Ellis & Martin) Sorauer in dual culture assay, with the inhibition rates of 61.39, 50.34 and 51.22%, respectively. Flocculated and dissolved hyphae of the phytopathogenic fungi were observed at the interaction zone on a dual-cultured PDA plate, but the hyphae of LB-1 were normal. The LB-1 cell-free filtrate has significant inhibitory effects on the three tested fungi in the poison plate assay; dissolved colonies, vesiculated and flocculated hyphae of the test pathogenic fungi were also found on the PDA plates supplemented with the LB-1 cell-free filtrate. Clear halo zones around the LB-1 colonies were found on the protease test plate, pectinase test plate and cellulose test plate, indicating that LB-1 could produce mucolytic enzymes of protease, pectinase and cellulase. However, the activities of chitinase and β-l,3-glucase were not detected on their test plates from LB-1. An obvious oil-displaced circle was formed in the oil spreading test, indicating that a surface-active substance might be contained in the LB-1 cell-free filtrate. These results proved that the biocontrol agent of C. subaffine LB-1 could exert its antifungal effects via living competition and mycolysis, and the latter may be obtained by production of mycolytic enzymes and surface-active substances.


Abdallah R.A.B., Mejdoub-Trabelsi B., Nefzi A., Jabnoun-Khiareddine H., Daami-Remadi M. (2016): Isolation of endophytic bacteria from Withania somnifera and assessment of their ability to suppress Fusarium wilt disease in tomato and to promote plant growth. Journal of Plant Pathology and Microbiology, 7: 1–11.
Abro M.A., Sun X., Li X., Jatoi G.H., Guo L.D. (2019): Biocontrol potential of fungal endophytes against Fusarium oxysporum f. sp. cucumerinum causing wilt in cucumber. The Plant Pathology Journal, 35: 598–608.
Aggarwal R., Tewari A.K., Srivastava K.D, Singh D.V. (2004): Role of antibiosis in the biological control of spot blotch (Cochliobolus sativus) of wheat by Chaetomium globosum. Mycopathologia, 157: 369–377.
Aktuganov G.E., Melent'ev A.I., Galimzyanova N.F., Shirokov A.V. (2008): The study of mycolytic properties of aerobic spore-forming bacteria producing extracellular chitinases. Microbiology, 77: 700–709.
Al-Askar A.A., Baka Z.A., Rashad Y.M., Ghoneem K.M., Abdulkhair W.M., Hafez E.E., Shabana Y.M. (2015): Evaluation of Streptomyces griseorubens E44G for the biocontrol of Fusarium oxysporum f. sp. lycopersici: Ultrastructural and cytochemical investigations. Annals of Microbiology, 65: 1815–1824.
Alfonso C., Santamar F.A., Nuerol O.M., Prleto A., Leal J.A., Reyes F. (1994): Biochemical studies on the cell wall degradation of Fusarium oxysporum f. sp. lycopersici race 2 by lytic enzymes from Mucorales for its biocontrol. Letters in Applied Microbiology, 18: 152–155.
Ashwini N., Srividya S. (2014): Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1. Biotech, 4: 127–136.
Chen Q., Liu B., Wang J., Che J., Liu G., Guan X. (2017): Antifungal lipopeptides produced by Bacillus sp. FJAT-14262 isolated from rhizosphere soil of the medicinal plant Anoectochilus roxburghii. Applied Biochemistry and Biotechnology, 182: 155–167.
Cook R.J. (1993): Making greater use of introduced microorganism for biological control of plant pathogens. Annual Review of Phytopathology, 31: 53–80.
Debono M., Gordee R.S. (1994): Antibiotics that inhibit fungal cell wall development. Annual Review of Microbiology, 48: 471–497.
Flores A., Chet I., Herrera-Estrella A. (1997): Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase-encoding gene prb1. Current Genetics, 31: 30–37.
Folman L.B., Postma J., van Veen J.A. (2003): Characterisation of Lysobacter enzymogenes (Christensen and Cook 1978) strain 3.1T8, a powerful antagonist of fungal diseases of cucumber. Microbiological Research, 158: 107–115.
Gao K., Liu X., Kang Z., Mendgen K. (2005): Mycoparasitism of Rhizoctonia solani by endophytic Chaetomium spirale ND35: Ultrastructure and cytochemistry of the interaction. Journal of Phytopathology, 153: 280–290.
Georgiou G., Lin S.C., Sharma M.M. (1992): Surface-active compounds from microorganism. Nature Biotechnology, 10: 60–65.
Guo X., Gao K.X., Yin J.M., Bai F.Q., Ma Y.X., Yu D., Liu X.G. (2005): Induction and characterization of β-1,3-glucanase from the mycoparasite Chaetomium spirale. Acta Phytopathologica Sinica, 35: 493–503 (in Chinese).
Hernandez-Montiel L.G., Gutiérrez-Pérez E.D., Murillo-Amador B., Verob S., Chiquito-Contreras R.G., Rincon-Enriquez G. (2018): Mechanisms employed by Debaryomyces hansenii in biological control of anthracnose disease on papaya fruit. Postharvest Biology and Technology, 139: 31–37.
Kikot G.E., Hours R.A., Alconada T.M. (2009): Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: A review. Journal of Basic Microbiology, 49: 231–241.
Liu C.Y., Xu R.R., Ji H.L., Chang Z. L. (2015): Isolation, screening and identification of an endophytic fungus and the detection of its antifungal effects. Journal of Plant Protection, 42: 806–812 (in Chinese).
Liu C.Y., Ji H.L., Wang R., Liu C.X. (2018): Antagonistic effects against several plant pathogenic fungi and the growth adaptability of a biocontrol strain LB-1. Journal of Plant Protection, 45: 332–339 (in Chinese).
Lu D., Ma Z., Xu X., Yu X. (2016): Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum. Journal of Basic Microbiology, 56: 929–933.
Maget-Dana R., Peypoux F. (1994): Iturins, a special class of pore-forming lipopeptides: Biological and physicochemical properties. Toxicology, 87: 151–174.
Markovich N., Kononova G. (2003): Lytic enzymes of Trichoderma and their role in plant defense from fungal diseases: A review. Applied Biochemistry and Microbiology, 39: 341–351.
McMullin D.R., Sumarah M.W., Blackwell B.A., Miller J.D. (2013): New azaphilones from Chaetomium globosum isolated from the built environment. Tetrahedron Letters, 54: 568–572.
Mitchell R., Alexander M. (1963): Lysis of soil fungi by bacteria. Canadian Journal of Microbiology, 9: 169–177.
Monte E. (2001): Understanding Trichoderma: Between biotechnology and microbial ecology. International Microbiology, 4: 1–4.
Narasimhan A., Shivakumar S. (2016): Biocontrol of Rhizoctonia solani root rot of chilli by Bacillus subtilis formulations under pot conditions. Journal of Biological Control, 30: 109–118.
Parafati L., Vitale A., Restuccia C., Cirvilleri G. (2015): Biocontrol ability and action mechanism of food-isolated yeast strains against Botrytis cinerea causing post-harvest bunch rot of table grape. Food Microbiology, 47: 85–92.
Park J.H., Choi G.J., Jang K.S., Lim H.K., Kim H.T., Cho K.Y., Kim J.C. (2005): Antifungal activity against plant pathogenic fungi of chaetoviridins isolated from Chaetomium globosum. FEMS Microbiology Letters, 252: 309−313.
Parmar H.J., Bodar N.P., Lakhani H.N., Patel S.V., Umrania V.V., Hassan M.M. (2015): Production of lytic enzymes by Trichoderma strains during in vitro antagonism with Sclerotium rolfsii, the causal agent of stem rot of groundnut. African Journal of Microbiology Research, 9: 365−372.
Phong N.H., Pongnak W., Soytong K. (2016): Antifungal activities of Chaetomium spp. against Fusarium wilt of tea. Plant Protection Science, 52: 10–17.
Prasad R.D., Navaneetha T., Rao L.V. (2016): Plant growth promotion and induced defense response in safflower (Carthamus tinctorius L.) by Trichoderma. Journal of Biological Control, 30: 40–48.
Ramot O., Cohen-Kupiec R., Chet I. (2000): Regulation of β-1,3-glucanase by carbon starvation in the mycoparasite Trichoderma harzianum. Mycological Research, 104: 415–420.
Ramzan N., Noreen N., Perveen Z., Shahzad S. (2016): Evaluation of enzymatic activities and degradation abilities of antagonistic microorganisms associated with compost. International Journal of Biological Biotechnology, 13: 135–141.
Roberts W.K., Selitrennikoff C.P. (1988): Plant and bacterial chitinases differ in antifungal activity. Microbiology, 134: 169–176.
Sahai A.S., Manocha M.S. (1993): Chitinases of fungi and plants: Their involvement in morphogenesis and host-parasite interaction. FEMS Microbiological Review, 11: 317–338.
Shanthiyaa V., Saravanakumar D., Rajendran L., Karthikeyan G., Prabakar K., Raguchander T. (2013): Use of Chaetomium globosum for biocontrol of potato late blight disease. Crop Protection, 52: 33–38.
Siameto E.N., Okoth S., Amugune N.O., Chege N.C. (2010): Antagonism of Trichoderma harzianum isolates on soil borne plant pathogenic fungi from Embu District, Kenya. Journal of Yeast and Fungal Research, 1: 47–54.
Sivan A., Chet I. (1989): Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. Journal of General Microbiology, 135: 675–682.
Song B., Rong Y. J., Zhao M. X., Chi Z. M. (2013): Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic. Applied Microbiology and Biotechnology, 97: 7141–7150.
Soytong K., Kanokmedhakul S., Kukongviriyapa V., Isobe M. (2001): Application of Chaetomium species (Ketomium®) as a new broad spectrum biological fungicide for plant disease control: A review article. Fungal Diversity, 7: 1–15.
Stephan D., Da Silva A.P.M., Bisutti I.L. (2016): Optimization of a freeze-drying process for the biocontrol agent Pseudomonas spp. and its influence on viability, storability and efficacy. Biological Control, 94: 74–81.
Vazquez-Garciduenas S., Leal-Morales C.A., Herrera-Estrella A. (1998): Analysis of the β-1,3-glucanolytic system of the biocontrol agent Trichoderma harzianum. Applied and Environmental Microbiology, 64: 1442–1446.
Viterbo A., Ramot O., Chernin L., Chet I. (2002): Significance of lytic enzymes from Trichoderma spp. in the biocontrol of fungal plant pathogens. Antonie van Leeuwenhoek, 81: 549–556.
Wang F., Lv S., Liu W.Q., Zeng L.S., Du C.X., Zhou J.K., Han X.X., Liu J.P. (2014): Screening of antagonistic bacteria against Fusarium oxysporum f. sp. cubense and analysis of the substances associated with biological control. Journal of Jiangxi Agriculture University, 36: 1264–1269 (in Chinese).
Wang S., Liang Y., Shen T., Yang H., Shen B. (2016): Biological characteristics of Streptomyces albospinus CT205 and its biocontrol potential against cucumber Fusarium wilt. Biocontrol Science and Technology, 26: 951–963.
Xu X.L., Huang X.L., Zhang C., Xu M.Q., Luo J., Liu Y.G. (2014): Selection of predominant endophytic Chaetomium spp. as biocontrol agents from spruce needles. Chinese Journal of Biological Control, 30: 511–519 (in Chinese).
Yamada T., Muroga Y., Jinno M., Kajimoto T., Usami Y., Numata A., Tanaka R. (2011): New class azaphilone produced by a marine fish-derived Chaetomium globosum. The stereochemistry and biological activities. Bioorganic & Medicinal Chemistry, 19: 4106−4113.
Youssef N.H., Duncan K.E., Nagle D.P., Savage K.N., Knapp R.M., Mclnerney M.J. (2004): Comparison of methods to detect biosurfactant production by diverse microorganisms. Journal of Microbiological Methods, 56: 339–347.
Zhang C., Liu Y., Zhu G. (2010): Detection and characterization of benzimidazole resistance of Botrytis cinerea in greenhouse vegetables. European Journal of Plant Pathology, 126: 509–515.
Zhu Z., Luo Y., Zhang P., Yang X.M., Ran W., Shen Q.R. (2011): Screening a surfactin and iturin A producing strain and characterization of its lipopeptide products. Microbiology China, 38: 1488–1498 (in Chinese).
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti