Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways

https://doi.org/10.17221/67/2014-PPSCitation:Degani O. (2015): Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways. Plant Protect. Sci., 51: 53-60.
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The role of G-protein and mitogen-activated protein kinase (MAPK) in filamentous fungi has been studied for over a decade, but downstream elements are less known. Here, we used microarray and Northern blot analysis to examine the involvement of these signalling pathways in controlling the knownCochliobolus heterostrophus T-toxin biosynthetic gene, DEC1, whose control is important in epidemic prevention. Comparison of the expression profile in wild-type strains and in G-protein and MAPK signalling deficient mutants revealed a unique, environmental-dependent control mechanism for the DEC1 gene. The results suggest, for the first time, a possible role in pathogenicity for the G-protein α2 subunit in this organism, and hint to a common role of G-protein α1 and β1 subunits and MAPK in maintaining accurate levels of this toxin during pathogenesis.
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