Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways O. (2015): Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways. Plant Protect. Sci., 51: 53-60.
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The role of G-protein and mitogen-activated protein kinase (MAPK) in filamentous fungi has been studied for over a decade, but downstream elements are less known. Here, we used microarray and Northern blot analysis to examine the involvement of these signalling pathways in controlling the knownCochliobolus heterostrophus T-toxin biosynthetic gene, DEC1, whose control is important in epidemic prevention. Comparison of the expression profile in wild-type strains and in G-protein and MAPK signalling deficient mutants revealed a unique, environmental-dependent control mechanism for the DEC1 gene. The results suggest, for the first time, a possible role in pathogenicity for the G-protein α2 subunit in this organism, and hint to a common role of G-protein α1 and β1 subunits and MAPK in maintaining accurate levels of this toxin during pathogenesis.
Agrios G.N. (2005): Plant Pathology. 5th Ed. London, Academic Press Inc., Ltd.
Altschul S. (): Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402
Ausubel F., Brent R., Kingston R., Moore D., Seidman J.G., Smith J.A., Struhl K. (1987): Current Protocols in Molecular Biology. New York, John Wiley Sons.
Bölker Michael (1998): Sex and Crime: Heterotrimeric G Proteins in Fungal Mating and Pathogenesis. Fungal Genetics and Biology, 25, 143-156
Carson M. L. (1998): Aggressiveness and Perennation of Isolates of Cochliobolus heterostrophus from North Carolina. Plant Disease, 82, 1043-1047
Church G. M., Gilbert W. (1984): Genomic sequencing.. Proceedings of the National Academy of Sciences, 81, 1991-1995
Clapham David E., Neer Eva J. (1993): New roles for G-protein (βγ-dimers in transmembrane signalling. Nature, 365, 403-406
Crespo Piero, Xu Ningzhi, Simonds William F., Gutkind J. Silvio (1994): Ras-dependent activation of MAP kinase pathway mediated by G-protein βγ subunits. Nature, 369, 418-420
Degani O. (2013a): Cochliobolus heterostrophus G-protein alpha and beta subunit double mutant reveals shared and distinct roles in development and virulence. Physiological and Molecular Plant Pathology, 82: 35–45.
Degani O. (2013b): Construction of a constitutively activated Gα mutant in the maize pathogen Cochliobolus heterostrophus. American Journal of Plant Sciences, 4: 2394–2399.
Degani O. (2014a): G protein and MAPK signaling pathways control the ability of Cochliobolus heterostrophus to exploit different carbon sources. Advances in Biological Chemistry, 4: 40–50.
Degani O. (2014b): Gene expression modulation of two biosynthesis pathways via signal transduction in Cochliobolus heterostrophus. Advances in Bioscience and Biotechnology, 5: 340-352.
Degani O., Maor R., Hadar R., Sharon A., Horwitz B. A. (): Host Physiology and Pathogenic Variation of Cochliobolus heterostrophus Strains with Mutations in the G Protein Alpha Subunit, CGA1. Applied and Environmental Microbiology, 70, 5005-5009
Degani Ofir, Lev Sophie, Ronen Mordechai (2013): Hydrophobin gene expression in the maize pathogen Cochliobolus heterostrophus. Physiological and Molecular Plant Pathology, 83, 25-34
Eliahu N., Igbaria A., Rose M. S., Horwitz B. A., Lev S. (): Melanin Biosynthesis in the Maize Pathogen Cochliobolus heterostrophus Depends on Two Mitogen-Activated Protein Kinases, Chk1 and Mps1, and the Transcription Factor Cmr1. Eukaryotic Cell, 6, 421-429
Ganem S., Lu S.-W., Lee B.-N., Chou D. Y.-T., Hadar R., Turgeon B. G., Horwitz B. A. (): G-Protein   Subunit of Cochliobolus heterostrophus Involved in Virulence, Asexual and Sexual Reproductive Ability, and Morphogenesis. Eukaryotic Cell, 3, 1653-1663
Gudermann T. (2008): Multiple pathways of ERK activation by G protein-coupled receptors. In: Bock G.R., Goode J.A.: Complexity in Biological Information Processing: Novartis Foundation Symposium, 239: 68–79; discussion 80–64, 150–159.
Horwitz Benjamin A., Sharon Amir, Lu Shun-Wen, Ritter Vladimir, Sandrock Tanya M., Yoder O.C., Turgeon B.Gillian (1999): A G Protein Alpha Subunit fromCochliobolus heterostrophusInvolved in Mating and Appressorium Formation. Fungal Genetics and Biology, 26, 19-32
Kodama M., Rose M.S., Yang G., Yun S.H., Yoder O.C., Turgeon B.G. (1999): The translocation-associated tox1 locus of Cochliobolus heterostrophus is two genetic elements on two different chromosomes. Genetics, 151: 585–596.
Leach J., Lang B. R., Yoder O. C. (1982): Methods for Selection of Mutants and In Vitro Culture of Cochliobolus heterostrophus. Microbiology, 128, 1719-1729
Lev S. (): A Mitogen-Activated Protein Kinase Pathway Modulates the Expression of Two Cellulase Genes in Cochliobolus heterostrophus during Plant Infection. THE PLANT CELL ONLINE, 15, 835-844
Lev S., Sharon A., Hadar R., Ma H., Horwitz B. A. (1999): A mitogen-activated protein kinase of the corn leaf pathogen Cochliobolus heterostrophus is involved in conidiation, appressorium formation, and pathogenicity: Diverse roles for mitogen-activated protein kinase homologs in foliar pathogens. Proceedings of the National Academy of Sciences, 96, 13542-13547
Matthews D. E., Gregory P., Gracen V. E. (1979): Helminthosporium maydis Race T Toxin Induces Leakage of NAD+ from T Cytoplasm Corn Mitochondria. PLANT PHYSIOLOGY, 63, 1149-1153
Rhoads David M., Levings Charles S., Siedow James N. (1995): URF13, a ligand-gated, pore-forming receptor for T-toxin in the inner membrane ofcms-T mitochondria. Journal of Bioenergetics and Biomembranes, 27, 437-445
Rose Mark S., Yun Sung-Hwan, Asvarak Thipa, Lu Shun-Wen, Yoder O. C., Turgeon B. Gillian (2002): A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize. Molecular Plant-Microbe Interactions, 15, 883-893
Siedow James N., Rhoads David M., Ward Gerty C., Levings Charles S. (1995): The relationship between the mitochondrial gene T-urf 13 and fungal pathotoxin sensitivity in maize. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1271, 235-240
Wildsmith S E (): Microarrays under the microscope. Molecular Pathology, 54, 8-16
Yang G. (): A Polyketide Synthase Is Required for Fungal Virulence and Production of the Polyketide T-Toxin. THE PLANT CELL ONLINE, 8, 2139-2150
Yoder O.C., Macko V., Wolpert T.J., Turgeon B.G. (1997): Cochliobolus spp. and their host-specific toxins. Berlin, Springer-Verlag.
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