Laboratory evaluation of the effect of the entomopathogenic fungi, Hirsutella thompsonii and Paecilomyces fumosoroseus, against the citrus brown mite, Eutetranychus orientalis (Acari: Tetranychidae

https://doi.org/10.17221/72/2014-PPSCitation:El-Sharabasy H.M. (2015): Laboratory evaluation of the effect of the entomopathogenic fungi, Hirsutella thompsonii and Paecilomyces fumosoroseus, against the citrus brown mite, Eutetranychus orientalis (Acari: Tetranychidae. Plant Protect. Sci., 51: 39-45.
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Formulations of the entomopathogenic fungi Hirsutella thompsonii (Fisher) and Paecilomyces fumosoroseus (Wize) Brown and Smith were tested against all stages of this pest under laboratory conditions. Three concentrations: 0.5 × 109, 1.0 × 109, and 2.0 × 10conidia/ml were sprayed on leaf discs containing larvae, nymphs, and adults, while a single dosage of 1.0 × 10conidia/ml wassprayed on eggs as ovicide. All the life stages were susceptible to both fungal pathogens at the tested concentrations. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis, H. thompsonii was the highest virulent with LC50 and LT50 (358, 290, and 146 conidia/ml and 7.78, 7.11, and 6.92 days) and P. fumosoroseus (597, 589, and 339 conidia/ml and 7.49, 7.14, and 4.31 days) for larvae, nymphs, and adults, respectively. However the efficacy of the two pathogens evaluated clearly differs from that of the controls. Compared with controls, egg hatchability was reduced at the different pathogen concentrations used. H. thompsonii at LC50 was more effective against eggs. Also, females in the control laid more eggs than those treated with the fungi. The entomopathogenic fungi H. thompsonii and P. fumosoroseus could be considered as an environmentally friendly alternative for biocontrol of E. orientalis.
References:
Abbott W. S. (1925): A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology, 18, 265-267  https://doi.org/10.1093/jee/18.2.265a
 
Afifi A.A.M., Mabrouk A.M., Asran A.A. (2010): Effect of the entomopathogenic fungus Beauveria bassiana on three acarine pests. In: Proceedings 12th International Congress of Acarology, Amsterdam, 21–26 Aug 2006: 439–440.
 
Alves Sérgio Batista, Rossi Luciana Savoi, Lopes Rogério Biaggioni, Tamai Marco Antonio, Pereira Roberto M (2002): Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea saccharalis (Lepidoptera: Crambidae) and Tetranychus urticae (Acari: Tetranychidae). Journal of Invertebrate Pathology, 81, 70-77  https://doi.org/10.1016/S0022-2011(02)00147-7
 
Amjad M., Bashir M.H., Afzal M., Sabri M.A., Javed N. (2012): Synergistic effect of some entomopathogenic fungi and synthetic pesticides, against two spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Pakistan Journal of Zoology, 44: 977–984.
 
El-Hady M.M. (2004): Susceptibility of the citrus brown mite, Eutetranychus orientalis (Klein) to the entomopathogenic fungi Verticillium lecanii and Metahrrizium anisopliae. Egyptian Journal of Biological Pest Control, 14: 409–410.
 
Finney D.J. (1971): Probit Analysis. 3rd Ed. Cambridge, Cambridge University Press.
 
Ghosh S.K., Shivaprakash T.H., Kaderr-Khan H. (2007): Susceptibility of two spotted red spider mites, Tetranychus urticae (Koch) (Acarina: Tetranychidae) against entomofungal pathogens. Journal of Biological Control, 21: 183–186.
 
Gupta Subhash C., Leathers Timothy D., El-Sayed Galal N., Ignoffo Carlo M. (1992): Insect cuticle-degrading enzymes from the entomogenous fungusBeauveria bassiana. Experimental Mycology, 16, 132-137  https://doi.org/10.1016/0147-5975(92)90019-N
 
Hanna A.I., Heikal I.H. (1995): Biological control of Tetranychus urticae Koch by the fungus Hirsutella thompsonii var. synnematosa on kidney bean plants in a greenhouse. Egyptian Journal of Applied Science, 10: 221–224.
 
Iskander N.G., Ibrahim S.M., Heikal I.H. (1993): Effect of certain chemicals on different developmental stages of some phytophagous and predaceous mite species. Egyptian Journal of Agricultural Research, 71: 453–461.
 
Kandeel M.M.H., Rakha M.A., El-Halaeany M.E. (1986): Citrus mites in Egypt. Agricultural Research Review (Cairo), 64(1): 132–127.
 
Kim J.S., Roh J.Y., Choi J.Y., Shin S.C., Jeon M.J., Je Y.H. (2008): Insecticidal activity of Paecilomyces fumosoroseus SFP-198 as a multi targeting biological control agent against the greenhouse whitefly and the two spotted spider mite. Internaltional Journal of Industrial Entomology, 17: 181–187.
 
Latgé J. P., Cabrera R. L. Cabrera, Prévost M. C. (1988): Microcycle conidiation in Hirsutella thompsonii. Canadian Journal of Microbiology, 34, 625-630  https://doi.org/10.1139/m88-103
 
Maniania Nguya K., Bugeme David M., Wekesa Vitalis W., Delalibera Italo, Knapp Markus (2008): Role of entomopathogenic fungi in the control of Tetranychus evansi and Tetranychus urticae (Acari: Tetranychidae), pests of horticultural crops. Experimental and Applied Acarology, 46, 259-274  https://doi.org/10.1007/s10493-008-9180-8
 
McCoy C.W. (1996): Pathogens of eriophyoid mites. In: Lindquist E.E., Sabelis M.W., Bruin J. (eds): Eriophyoid Mites – Their Biology. Natural Enemies and Control. Amsterdam, Elsevier: 481–490.
 
Pena J.E., Osborne L.S., Duncan R.E. (1996): Potential of fungi as biocontrol agents of Polyphagotarsonemus latus (Acari: Tarsonemidae). Entomophaga, 41: 27–36.
 
Rasmy A.H. (1978): Biology of citrus brown mite, Eute-tranychus orientalis (Klein) as affected by some citrus species. Acarologia, 19: 222–224.
 
Rodrigues-Reudan D., Fargues J. (1980): Pathogenicity of entomopathogenic hyphomycetes. Paecilomyces fumosoroseus and Nomuraea rileyi, to eggs of noctuids, Mamestra brassicae and Spodoptera littoralis. Journal of Invertebrate Pathology, 36: 339–408.
 
Samish Michael, Gindin Galina, Alekseev Evgeny, Glazer Itamar (2001): PATHOGENICITY OF ENTOMOPATHOGENIC FUNGI TO DIFFERENT DEVELOPMENTAL STAGES OF RHIPICEPHALUS SANGUINEUS (ACARI: IXODIDAE). Journal of Parasitology, 87, 1355-1359  https://doi.org/10.1645/0022-3395(2001)087[1355:POEFTD]2.0.CO;2
 
SAS Institute (1990): SAS/STAT User’s Guide, Version 6. 4th Ed. Vol. 2. Cary, SAS Institute.
 
Shaw Katie E, Davidson Gillian, Clark Suzanne J, Ball Brenda V, Pell Judith K, Chandler David, Sunderland Keith D (2002): Laboratory bioassays to assess the pathogenicity of mitosporic fungi to Varroa destructor (Acari: Mesostigmata), an ectoparasitic mite of the honeybee, Apis mellifera. Biological Control, 24, 266-276  https://doi.org/10.1016/S1049-9644(02)00029-4
 
Shi W.B., Feng M.G. (2004a): Lethal effect of Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces fumosoroseus on the eggs of Tetranychus cinnabarinus (Acari: Tetranychidae) with a description of a mite egg bioassay system. Biological Control, 30: 165–173.
 
Shi W.B., Feng M.G. (2004b): Ovicidal activity of two fungal pathogens (Hyphomycetes) against Tetranychus cinnabarinus (Acarina: Tetranichidae). Chinese Science Bulletin, 49: 263–267
 
Shi Wei-Bing, Feng Ming-Guang (2009): Effect of fungal infection on reproductive potential and survival time of Tetranychus urticae (Acari: Tetranychidae). Experimental and Applied Acarology, 48, 229-237  https://doi.org/10.1007/s10493-009-9238-2
 
Sewify G.H., Mabrouk A.M. (1991): The susceptibility of different stages of the citrus brown mite Eutetranychus orientalis (Klein) (Acarina: Tetranychidae) to the entomopathogenic fungus Verticillium lecanii (Zimm) Viegas. Egyptian Journal of Applied Biology and Control, 1: 89–92.
 
Wekesa V.W., Maniania N.K., Knapp M., Boga H.I. (2005): Pathogenicity of Beauveria bassiana and Metarhizium anisoplae to the tobacco spider mite Tetranychus evansi. Experimental & Applied Acarology, 36: 41–50.
 
Wekesa V. W., Knapp M., Maniania N. K., Boga H. I. (2006): Effects of Beauveria bassiana and Metarhizium anisopliae on mortality, fecundity and egg fertility of Tetranychus evansi. Journal of Applied Entomology, 130, 155-159  https://doi.org/10.1111/j.1439-0418.2006.01043.x
 
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