A new disease of strawberry, fruit rot, caused by Geotrichum candidum in China.

https://doi.org/10.17221/76/2017-PPSCitation:Ma W., Zhang Y., Wang C., Liu S., Liao X. (2018): A new disease of strawberry, fruit rot, caused by Geotrichum candidum in China. Plant Protect. Sci., 54: 92-100.
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A new disease of strawberry (Fragaria ananassa Duch.) was discovered in the Lianqiao strawberry planting base in Shaodong County, in Hunan Province, China. In the early disease stage, leaves showed small black spots surrounded by yellow halos, while in the late stage, a white fluffy layer of mold appeared. Fruits were covered with a white layer of mold. The symptoms were observed using in vitro inoculation experiments. After the spray-inoculation of stabbed leaves, small black spots surrounded by yellow halos occurred on leaves, with no clear boundary between diseased and healthy areas. In the late stage, disease spots gradually expanded and a white fluffy layer of mold formed under humid conditions. Unstabbed leaves had almost no disease occurrence after spray-inoculation. After the spray-inoculation of stabbed fruits, by the late stage, a dense white layer of mold formed. According to Koch’s postulates, the isolated strain was verified as a pathogen. The pathogenic strain, designated SDLQ16, was isolated from diseased fruit by dilution method and tentatively identified as G. candidum based on the culture characteristics, morphologies, physio-biochemical analysis, and phylogenetic analysis of the rDNA-ITS sequence. The fungus was able to grow on different culture mediums, with a broad range of nutrition. The colonies on PDA medium were raised and pale white, with a neat edge and visible hyphae. The hyphae were friable but the spores were developing. Basal hyphae rapidly grew close to the medium to 3.2–4.2 µm in diameter, with septa and forked branches at acute angles. The solitary or beaded spores with smooth surfaces were 3.5–7.5 µm in length and 3.5–4.5 µm in width. This strain was able to gelatin liquefaction, proteolysis, grease, peptonised milk, urea, and so on. The pathogenicity on strawberry from strong to weak was: fruit > leaf > stem. A BLAST algorithm was used to query SDLQ16’s rDNA-ITS sequence (cloned and deposited as GenBank number KU373122) against the NCBI database, and it was located in the Acinetobacter sp. branch of a phylogenetic tree. SDLQ16 was most closely related to Geotrichum candidum ATCC34614 (GQ4580314.1), with a sequence similarity of 99%.

Asad-Uz-Zaman Md, Bhuiyan Mohammad Rejwan, Khan Mohammad Ashik Iqbal, Alam Bhuiyan Md Khurshed, Latif Mohammad Abdul (2015): Integrated options for the management of black root rot of strawberry caused by Rhizoctonia solani Kuhn. Comptes Rendus Biologies, 338, 112-120 https://doi.org/10.1016/j.crvi.2014.11.006
Barnett H.L., Hunter B.B. (1998): Illustrated Genera of Imperfect Fungi. 4th Ed. Minnesota, Burgess Publishing Co.: 34–35.
Behrouz E.M., Marie T.C., Odile C. (2006): Superoxide dismutase responses of strawberry cultivars to infection by Mycosphaerella fragariae. Journal of Plant Physiology, 2: 147–153.
Blair Jaime E., Coffey Michael D., Park Sook-Young, Geiser David M., Kang Seogchan (2008): A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences. Fungal Genetics and Biology, 45, 266-277 https://doi.org/10.1016/j.fgb.2007.10.010
Bourret T.B., Kramer E.K., Rogers J.D., Glawe D.A. (2013): Isolation of Geotrichum candidum pathogenic to tomato (Solanum lycopersicum) in Washington State. North American Fungi, 14: 1–7.
Campos-Requena Víctor H., Rivas Bernabé L., Pérez Mónica A., Figueroa Carlos R., Sanfuentes Eugenio A. (2015): The synergistic antimicrobial effect of carvacrol and thymol in clay/polymer nanocomposite films over strawberry gray mold. LWT - Food Science and Technology, 64, 390-396 https://doi.org/10.1016/j.lwt.2015.06.006
ECKERT JOSEPH W. (1978): PATHOLOGICAL DISEASES OF FRESH FRUITS AND VEGETABLES. Journal of Food Biochemistry, 2, 243-250 https://doi.org/10.1111/j.1745-4514.1978.tb00619.x
Eikemo Håvard, Stensvand Arne (2015): Resistance of strawberry genotypes to leather rot and crown rot caused by Phytophthora cactorum. European Journal of Plant Pathology, 143, 407-413 https://doi.org/10.1007/s10658-015-0685-9
Fang Z.D. (1998): The Research Methods of Plant Pathology. 3rd Ed. Beijing, China Agriculture Press: 45–50.
Fang Xianping, Chen Wenyue, Xin Ya, Zhang Hengmu, Yan Chengqi, Yu Hong, Liu Hui, Xiao Wenfei, Wang Shuzhen, Zheng Guizhen, Liu Hongbo, Jin Liang, Ma Huasheng, Ruan Songlin (2012): Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae. Journal of Proteomics, 75, 4074-4090 https://doi.org/10.1016/j.jprot.2012.05.022
FAOSTAT (2013): Searchable online statistical database from Food and Agriculture Division of the United Nations. Available at http://faostat.fao. org/site/567/DesktopDefault.aspx?PageID=567#ancor (accessed June 24, 2014).
Gao B.-D., Huang W., Xia H. (2012): A New Rice Disease, Black Sheath Spot, Caused by Curvularia fallax in China. Plant Disease, 96, 1224-1224 https://doi.org/10.1094/PDIS-01-12-0021-PDN
Horita Harukuni, Hatta Yo (2016): Sour rot of carrot caused by Geotrichum candidum in Japan. Journal of General Plant Pathology, 82, 65-68 https://doi.org/10.1007/s10327-015-0638-3
Lachhab N., Sanzani S.M., Bahouaoui M.A., Boselli M., Ippolito A. (2015): Effect of some protein hydrolysates against gray mould of table and wine grapes. European Journal of Plant Pathology, 9: 1–10.
Li T.H., Wang L. (2004): The situation of production and trade and strategy analysis of the sustainable development of chinese strawberry industry. Chinese Agricultural Science Bulletin, 6: 372–375.
Li Y., Jin W., Huang J.W. (2005): Identification and biological characteristics of Sclerotium rolfsii Sacc causing southern blight on strawberry. Journal of Huazhong Agricultural University, 3: 250–253
Liu S.H., Lu J.P., Zhu R.L., Dai F.M. (2005): A rapid and simple extraction method for plant pathogenic fungi. Acta Phytopathologica Sinica, 4: 362–365.
Liu T.M., Wang H.H., Wang J.J. (2011): Relationship analysis between genetic diversity of Geotrichum candidum and geographical regions. Biotechnology Bulletin, 5: 121–125.
Lv R.L., Xie J.T., Fu Y.P., Jiang D.H. (2010): Identification of pathogen that causes strawberry brown blotch disease and observation of biological characteristics. Journal of Huazhong Agricultural University, 4: 427–430.
Ma K., Liu G.Q., Li J.X., Yao S., Cheng C. (2007): The phylogenetic analysis of 15 Geotrichum strains based on 26S rRNA gene D1/D2 region sequencing. Acta Microbiologica Sinica, 2: 359–362.
Moreno-Garcia S., Grey O. (eds) (2012): Fungi. Vancouver, Innsmouth Free Press.
Šamec Dunja, Maretić Marina, Lugarić Ivana, Mešić Aleksandar, Salopek-Sondi Branka, Duralija Boris (2016): Assessment of the differences in the physical, chemical and phytochemical properties of four strawberry cultivars using principal component analysis. Food Chemistry, 194, 828-834 https://doi.org/10.1016/j.foodchem.2015.08.095
Sun D.W., Cao J.F., Pei W.H., Yin G.F., Ma J.Q., Pan K.H., Wu K., Zhao Q., Duan Z.Q., Yang M.Y., Wang L., Zhao Z.J. (2015): Identification of a new phytophthora blight disease on konjac in Yunnan. Acta Phytopathologica Sinica, 1: 84–87.
Sylla Justine, Alsanius Beatrix W., Krüger Erika, Becker Dorit, Wohanka Walter (2013): In vitro compatibility of microbial agents for simultaneous application to control strawberry powdery mildew (Podosphaera aphanis). Crop Protection, 51, 40-47 https://doi.org/10.1016/j.cropro.2013.04.011
Talibi I., Askarne L., Boubaker H., Boudyach E.H., Msanda F., Saadi B., Ait Ben Aoumar A. (2012): Antifungal activity of Moroccan medicinal plants against citrus sour rot agent Geotrichum candidum. Letters in Applied Microbiology, 55, 155-161 https://doi.org/10.1111/j.1472-765X.2012.03273.x
Takahashi H., Yamasaki A., Shoji K., Kawagishi K., Taguchi T., Yoshida Y., Morishita M. (2009): PRESENT STATUS AND PROSPECTS OF STRAWBERRY BREEDING AND CROPPING TYPE IN NORTHERN JAPAN. Acta Horticulturae, , 475-478 https://doi.org/10.17660/ActaHortic.2009.842.96
Tan C.H., Dai H.P., Lei J.J. (2003): Development trend of strawberry production and trade in the world. World Agriculture, 5: 10–12.
Wang G., Zhang Y.T., Dong J., Zhang L.X. (2008a): Retrospection and prospect of strawberry breeding in China. Journal of Plant Genetic Resources, 2: 272–276.
Wang F., Ma Y., Gao X.Y., Zhang Z.H. (2008b): Study on the identification techniques for determining strawberry cultivar’ s resistance to anthracnose. Journal of Fruit Science, 4: 542–547.
Walker Anne-Sophie, Gautier Angélique, Confais Johann, Martinho Daniel, Viaud Muriel, Le Pêcheur Pascal, Dupont Joelle, Fournier Elisabeth (2011): Botrytis pseudocinerea , a New Cryptic Species Causing Gray Mold in French Vineyards in Sympatry with Botrytis cinerea. Phytopathology, 101, 1433-1445 https://doi.org/10.1094/PHYTO-04-11-0104
Wei J.C. (1979): Determinative Manual of Common Fungi System. Shanghai, Science Press: 15–20. (in Chinese)
Yaghmour Mohammad A., Bostock Richard M., Morgan David P., Michailides Themis J. (2012): Biology and Sources of Inoculum of Geotrichum candidum Causing Sour Rot of Peach and Nectarine Fruit in California. Plant Disease, 96, 204-210 https://doi.org/10.1094/PDIS-05-11-0391
Zhang J., Zhang L., Li G.Q., Yang L., Jiang D.H., Zhuang W.Y., Huang H.C. (2010a): Botrytis sinoallii: a new species of the grey mould pathogen on Allium crops in China. Mycoscience, 6: 421–431.
Zhang J., Wu M.D., Li G.Q., Yang L., Yu L., Jiang D.H. (2010b): Botrytis fabiopsis, a new species causing chocolate spot of broad bean in central China. Mycologia, 5: 1114–1126.
Zhao M.Z., Wang J., Wang Z.W., Qian Y.M., Wu W.M. (2012): Countermeasures of strawberry sustainable development in Sanghai Zhejiang Jiangsu province and development status of strawberry industry in the world. Jiangsu Academy of Agricultural Sciences, 2: 1–3.
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