Bioassay and phylogeny of five Iranian isolates of Cucumber mosaic virus from different hosts based on CP gene sequence A., Dizadji A., Rastgou M., Koohi Habibi M. (2015): Bioassay and phylogeny of five Iranian isolates of Cucumber mosaic virus from different hosts based on CP gene sequence. Plant Protect. Sci., 51: 200-207.
download PDF
Using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), Cucumber mosaic virus (CMV) was detected in 31 out of 132 symptomatic leaf samples collected from different hosts of Urmia province of Iran, during 2011–2012. In biological assays, five different host isolates caused severe mosaic in Nicotiana species and Capsicum annum without significant difference in severity. Based on phylogenetic analysis of coat protein nucleotide and deduced amino acid sequence, two isolates were clustered into subgroup IA, while other three isolates were grouped into IB subgroup of CMV. All Urmian isolates shared a common MspI, and no EcoRI and BsuRI restriction sites. In contrast to S-IA isolates, the second MspI site was found at 473–476 position of only S-IB isolates, which could be used to differentiate two S-IA and S-IB subgroups. Here, we report the first case of Abutilon theophrasti infection, as a new reservoir weed host for CMV in the world.
Arafati Nazanin, Farzadfar Shirin, Pourrahim Reza (2013): Characterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran. Iranian Journal of Biotechnology, 11, x-x
Bashir Nemat Sokhandan, Kalhor Mohammad Rasaei, Zarghani Shaheen Nourinejhad (2006): Detection, Differentiation and Phylogenetic Analysis of Cucumber Mosaic Virus Isolates from Cucurbits in the Northwest Region of Iran. Virus Genes, 32, 277-288
Bashir N.S, Nematollahi S., Torabi E. (2008): Cucumber mosaic virus subgroup IA frequently occurs in the Northwest Iran. Acta Virologica, 52: 237–242.
Berniak Hanna, Kamińska Maria, Malinowski Tadeusz (2010): Cucumber mosaic virus groups IA and II are represented among isolates from naturally infected lilies. European Journal of Plant Pathology, 127, 305-309
Clark M. F., Adams A. N. (1977): Characteristics of the Microplate Method of Enzyme-Linked Immunosorbent Assay for the Detection of Plant Viruses. Journal of General Virology, 34, 475-483
Deyong Zhang, Willingmann Peter, Heinze Cornelia, Adam Günter, Pfunder Monica, Frey Beatrice, Frey Jürg E. (2005): Differentiation of Cucumber mosaic virus isolates by hybridization to oligonucleotides in a microarray format. Journal of Virological Methods, 123, 101-108
Haase Anita, Richter J., Rabenstein F. (1989): Monoclonal Antibodies for Detection and Serotyping of Cucumber Mosaic Virus. Journal of Phytopathology, 127, 129-136
Hayakawa T., Mizukami M., Nakajima M., Suzuki M. (1989): Complete Nucleotide Sequence of RNA 3 from Cucumber Mosaic Virus (CMV) Strain O: Comparative Study of Nucleotide Sequences and Amino Acid Sequences among CMV Strains O, Q, D and Y. Journal of General Virology, 70, 499-504
Hellwald K.H., Glenewinkle D., Hauber S., Pfanzenk Z. (2000): Increased symptom severity in pepper plants after co-inoculation with two Cucumber mosaic virus subgroup I strains in comparison to single inoculations of the corresponding wildtype strains. Journal of Plant Disease and Protection, 107: 368–375.
King A.M.Q., Adams M.J., Carstens E.B., Lefkowitz E.J. (2012): Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses. San Diego, Elsevier Academic Press.
Lin H.-X., Rubio L., Smythe A. B., Falk B. W. (): Molecular Population Genetics of Cucumber Mosaic Virus in California: Evidence for Founder Effects and Reassortment. Journal of Virology, 78, 6666-6675
Nematollahi Sevil, Sokhandan-Bashir Nemat, Rakhshandehroo Farshad, Zamanizadeh Hamid Reza (2012): Phylogenetic Analysis of New Isolates of Cucumber mosaic virus from Iran on the Basis of Different Genomic Regions. The Plant Pathology Journal, 28, 381-389
Nolasco G., De Blas C., Torres V., Ponz F. (1993): A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. Journal of Virological Methods, 45, 201-218
Palukaitis P, Garcia-Arenal F. (2003): Cucumber mosaic virus. Description of Plant Viruses. Availabe at
Palukaitis P., Roossinck M.J., Dietzgen R.G., Francki R.I.B. (1992): Cucumber mosaic virus. Advances in Virus Research, 41: 281–348.
Pares R.D., Gillings M.R., Gunn L.V. (1992): Differentiation of biologically distinct Cucumber mosaic virus isolates by PAGE of double-stranded RNA. Intervirology, 34: 23–29.
Rizos H., Gunn L. V., Pares R. D., Gillings M. R. (1992): Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Virology, 73, 2099-2103
Roossinck M. J. (2002): Evolutionary History of Cucumber Mosaic Virus Deduced by Phylogenetic Analyses. Journal of Virology, 76, 3382-3387
Roossinck M.J., Zhang L., Hellwald K.H. (1999): Rearrangements in the 5' nontranslated region and phylogenetic analyses of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. Journal of Virology, 73: 6752–6758.
Saitou N., Nei M. (1987): The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406–425.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (): MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739
Wilson A. D. (1985): Characterization and Field Studies of a Cucumber Mosaic Virus Isolate from Spinach in the Winter Garden Area of Texas. Plant Disease, 69, 751-
Yu Cui, Wu Jianxiang, Zhou Xueping (2005): Detection and subgrouping of Cucumber mosaic virus isolates by TAS-ELISA and immunocapture RT-PCR. Journal of Virological Methods, 123, 155-161
Zhang L., Hanada K., Palukaitis P. (1994): Mapping Local and Systemic Symptom Determinants of Cucumber Mosaic Cucumovirus in Tobacco. Journal of General Virology, 75, 3185-3191
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti