Synergism of entomopathogenic fungus, Metarhizium anisopliae incorporated with fipronil against oil palm pest subterranean termite, Coptotermes curvignathus

https://doi.org/10.17221/82/2015-PPSCitation:Yii J.E., Bong C.F.J., King J.H.P., Kadir J. (2016): Synergism of entomopathogenic fungus, Metarhizium anisopliae incorporated with fipronil against oil palm pest subterranean termite, Coptotermes curvignathus. Plant Protect. Sci., 52: 35-44.
download PDF
The compatibility and synergy in efficacy of the termiticide fipronil with a biocontrol agent Metarhizium anisopliae, alone or in combination, against the subterranean termite, Coptotermes curvignathus were investigated. Sublethal doses of fipronil were found relatively less detrimental to fungal growth of M. anisopliae local isolates in a compatibility test. The fungus–insecticide bait formulation showed the greatest synergistic effect that increased termite mortality as well as reduced the lethal time at a sublethal dose of 0.05 mg a.i./l fipronil with conidia concentrations of 107 conidia/g bait (χ² = 48.80) at LT50 value of 6.46 days, followed by 108 conidia/gbait (χ² = 5.09) at LT50 value of 4.89 days compared to the use of these control agents alone. The insecticidal stress caused by sublethal fipronil in the formulated bait may weaken the termites and reduce their defence mechanism, which facilitates fungus infection on termites. The observed synergism treatments show the potential for integrated fungus–insecticide control method and need to be further investigated on termite infested oil palm trees.
References:
Abbott W. S. (1925): A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology, 18, 265-267  https://doi.org/10.1093/jee/18.2.265a
 
Alves S.B., Moino Jr. A., Almeida J.E.M. (1998): Produtos fitossanitários e entomopatógenos. In: Alves S.B. (ed.): Controle microbiano de insetos. São Paulo, Fealq: 269–289.
 
Anderson T. E., Roberts D. W. (1983): Compatibility of Beauveria bassiana Isolates with Insecticide Formulations Used in Colorado Potato Beetle (Coleoptera: Chrysomelidae) Control. Journal of Economic Entomology, 76, 1437-1441  https://doi.org/10.1093/jee/76.6.1437
 
Anderson T. E., Hajek A. E., Roberts D. W., Preisler H. K., Robertson J. L. (1989): Colorado Potato Beetle (Coleoptera: Chrysomelidae): Effects of Combinations of Beauveria bassiana with Insecticides. Journal of Economic Entomology, 82, 83-89  https://doi.org/10.1093/jee/82.1.83
 
Asi M.R., Bashir M.H., Afzal M., Ashfaq M., Sahi S.T. (2010): Compatibility of entomopathogenic fungi, Metarhizium anisopliae and Paecilomyces fumosoroseus with selective insecticides. Pakistan Journal of Botany, 42: 4207–4214.
 
Chouvenc Thomas, Su Nan-Yao (2010): Apparent Synergy Among Defense Mechanisms in Subterranean Termites (Rhinotermitidae) Against Epizootic Events: Limits and Potential for Biological Control. Journal of Economic Entomology, 103, 1327-1337  https://doi.org/10.1603/EC09407
 
Chouvenc Thomas, Su Nan-Yao, Hughes William (2012): When Subterranean Termites Challenge the Rules of Fungal Epizootics. PLoS ONE, 7, e34484-  https://doi.org/10.1371/journal.pone.0034484
 
Chouvenc T., Su N.-Y., Elliott M. L. (2008): Interaction Between the Subterranean Termite Reticulitermes flavipes (Isoptera: Rhinotermitidae) and the Entomopathogenic Fungus Metarhizium anisopliae in Foraging Arenas. Journal of Economic Entomology, 101, 885-893  https://doi.org/10.1093/jee/101.3.885
 
Chouvenc T., Efstathion C.A., Elliott M.L., Su N.Y. (2012a): Resource competition between two fungal parasites in subterranean termites. Naturwissenschaften, 99: 949–958.
 
Chouvenc T., Efstathion C. A., Elliott M. L., Su N.-Y. (): Extended disease resistance emerging from the faecal nest of a subterranean termite. Proceedings of the Royal Society B: Biological Sciences, 280, 20131885-20131885  https://doi.org/10.1098/rspb.2013.1885
 
Chouvenc T., Robert A., Sémon E., Bordereau C. (2012b): Burial behaviour by dealates of the termite Pseudacanthotermes spiniger (Termitidae, Macrotermitinae) induced by chemical signals from termite corpses. Insectes Sociaux, 59: 119–125.
 
Chouvenc Thomas, Su Nan-Yao, Kenneth Grace J. (2011): Fifty years of attempted biological control of termites – Analysis of a failure. Biological Control, 59, 69-82  https://doi.org/10.1016/j.biocontrol.2011.06.015
 
Chouvenc T., Su N.Y., Robert A. (2009a): Cellular encapsulation in the eastern subterranean termite, Reticulitermes flavipes (Isoptera), against infection by the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, 101: 234–241.
 
Chouvenc T., Su N.Y., Robert A. (2009b): Inhibition of Metarhizium anisopliae in the alimentary tract of the eastern subterranean termite Reticulitermes flavipes. Journal of Invertebrate Pathology, 101: 130–136.
 
Chouvenc T., Su N.Y., Robert A. (2009c): Susceptibility of seven termite species (Isoptera) to the entomopathogenic fungus Metarhizium anisopliae. Sociobiology, 54: 723–748.
 
Dayakar S., Kanaujia K.R., Rathore R.R.S. (2000): Compatibility of entomogenous fungi with commonly used insecticides for the management of Spodoptera litura (Fab.). In: Ignacimuthu S., Sen A. (eds): Microbials in Insect Pest Management. Oxford and IBH Publishing Co. Delhi, Kolkata: 47–52.
 
Ericsson J. D., Todd Kabaluk J., Goettel M. S., Myers J. H. (2007): Spinosad Interacts Synergistically with the Insect Pathogen Metarhizium anisopliae Against the Exotic Wireworms Agriotes lineatus and Agriotes obscurus (Coleoptera: Elateridae). Journal of Economic Entomology, 100, 31-38  https://doi.org/10.1093/jee/100.1.31
 
Hiromori Hajime, Nishigaki Jojiro (2001): Factor analysis of synergistic effect between the entomopathogenic fungus Metarhizium anisopliae and synthetic insecticides.. Applied Entomology and Zoology, 36, 231-236  https://doi.org/10.1303/aez.2001.231
 
Hoe Pik-Kheng, J. Bong Choon-Fah, Jugah Kadir, Rajan Amartalingam (2009): Evaluation of Metarhizium anisopliae var. anisopliae (Deuteromycotina: Hyphomycete) Isolates and their Effects on Subterranean Termite Coptotermes curvignathus (Isoptera: Rhinotermitidae). American Journal of Agricultural and Biological Sciences, 4, 289-297  https://doi.org/10.3844/ajabssp.2009.289.297
 
Inglis G.D., Goettel M.S., Butt T.M., Strasser H. (2001): Use of hyphomycetous fungi for managing insect pests. In: Butt T.M., Jackson C., Magan N. (eds): Fungi as Biocontrol Agents: Progress, Problems and Potential. Wallingford, CAB International: 23–69.
 
Lim K.H., Silek B. (2001): Termite infestations on oil palms planted on deep peat in Sarawak: Tradewinds experience. In: Proceedings 2001 PIPOC International Palm Oil Congress, Agriculture Conference, Kuala Lumpur 2001 – Cutting-edge Technology for Sustained Competitiveness. MPOB, Kuala Lumpur: 355–368.
 
Luan Fenggang, Zhang Shengli, Wang Bin, Huang Bo, Li Zengzhi (2013): Genetic diversity of the fungal pathogen Metarhizium spp., causing epizootics in Chinese burrower bugs in the Jingting Mountains, eastern China. Molecular Biology Reports, 40, 515-523  https://doi.org/10.1007/s11033-012-2088-8
 
Mburu D.M., Ochola L., Maniania N.K., Njagi P.G.N., Gitonga L.M., Ndung’u M.W., Wanjoya A.K., Hassanali A. (2009): Relationship between virulence and repellency of entomopathogenic isolates of Metarhizium anisopliae and Beauveria bassiana to the termite Macrotermes michaelseni. Journal of Insect Physiology, 55, 774-780  https://doi.org/10.1016/j.jinsphys.2009.04.015
 
Milner R.J, Staples J.A, Lutton G.G (1998): The Selection of an Isolate of the Hyphomycete Fungus,Metarhizium anisopliae,for Control of Termites in Australia. Biological Control, 11, 240-247  https://doi.org/10.1006/bcon.1997.0574
 
Moino Jr. Alcides, Alves Sérgio B. (1998): Efeito de imidacloprid e fipronil sobre Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) Sorok. e no comportamento de limpeza de Heterotermes tenuis (Hagen). Anais da Sociedade Entomológica do Brasil, 27, 611-619  https://doi.org/10.1590/S0301-80591998000400014
 
NEVES PEDRO M.O.J., HIROSE EDSON, TCHUJO PAULO T., MOINO JR ALCIDES (2001): Compatibility of entomopathogenic fungi with neonicotinoid insecticides. Neotropical Entomology, 30, 263-268  https://doi.org/10.1590/S1519-566X2001000200009
 
Pell J.K., Eilenberg J., Hajek A.E., Steinkraus D.C. (2001): Biology, ecology and pest management potential of Entomophthorales. In: Butt T.M., Jackson C., Magan N. (eds): Fungi as Biocontrol Agents: Progress, Problems and Potential. Wallingford, CAB International: 71–153.
 
Ramakrishnan R., Suiter D. R., Nakatsu C. H., Humber R. A., Bennett G. W. (1999): Imidacloprid-Enhanced Reticulitermes flavipes (Isoptera: Rhinotermitidae) Susceptibility to the Entomopathogen Metarhizium anisopliae. Journal of Economic Entomology, 92, 1125-1132  https://doi.org/10.1093/jee/92.5.1125
 
Rath Andrew C. (2000): The Use of Entomopathogenic Fungi for Control of Termites. Biocontrol Science and Technology, 10, 563-581  https://doi.org/10.1080/095831500750016370
 
Schumacher Verona, Poehling Hans-Michael (2012): In vitro effect of pesticides on the germination, vegetative growth, and conidial production of two strains of Metarhizium anisopliae. Fungal Biology, 116, 121-132  https://doi.org/10.1016/j.funbio.2011.10.007
 
Shaw Katie E, Davidson Gillian, Clark Suzanne J, Ball Brenda V, Pell Judith K, Chandler David, Sunderland Keith D (2002): Laboratory bioassays to assess the pathogenicity of mitosporic fungi to Varroa destructor (Acari: Mesostigmata), an ectoparasitic mite of the honeybee, Apis mellifera. Biological Control, 24, 266-276  https://doi.org/10.1016/S1049-9644(02)00029-4
 
Sierotzki H., Camastral F., Shah P.A., Aebi M., Tuor U. (2000): Biological characteristics of selected Erynia neoaphidis isolates. Mycological Research, 104, 213-219  https://doi.org/10.1017/S0953756299001392
 
Silva Rodrigo Alves da, Quintela Eliane Dias, Mascarin Gabriel Moura, Barrigossi José Alexandre Freitas, Lião Luciano Moraes (2013): Compatibility of conventional agrochemicals used in rice crops with the entomopathogenic fungus Metarhizium anisopliae. Scientia Agricola, 70, 152-160  https://doi.org/10.1590/S0103-90162013000300003
 
Wakil W., Yasin M., Qayyuan M.A., Asim M. (2012): Combined toxicity of Metarhizium anisopliae with sublethal doses of chlorpyrifos, fipronil and chlorantraniliprole against Periplaneta americana (Dictyoptera: Blattidae). Pakistan Entomologist, 34: 59–63.
 
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti