Distribution and molecular identification of Meloidogyne spp. parasitising flue-cured tobacco in Yunnan, China

https://doi.org/10.17221/82/2017-PPSCitation:Zeng J., Zhang Z., Li M., Wu X., Zeng Y., Li Y. (2018): Distribution and molecular identification of Meloidogyne spp. parasitising flue-cured tobacco in Yunnan, China. Plant Protect. Sci., 54: 183-189.
download PDF

Twenty-one populations of root-knot nematodes (RKNs) were recovered from diseased roots collected from flue-cured tobacco in 21 locations in Yunnan (China) during 2014–2015. Molecular diagnosis on species was performed based on characteristics of sequences of D2-D3 expansion domains of the 28S rDNA and sequence characterised amplified regions (SCAR). SCAR results identified 13 populations as Meloidogyne arenaria, six as M. javanica and two as M. incognita. Meloidogyne arenaria is the predominant species with a detection rate of 61.9%, followed by M. javanica (28.5%) and M. incognita (9.5%). The tobacco RKNs were mainly distributed in western, central, and southwestern Yunnan, particularly in the western areas. Meloidogyne arenaria and M. javanica mainly occurred in Lincang (southwestern Yunnan) and Yuxi (central Yunnan), and M. incognita only in Zhaotong (northeastern Yunnan). Phylogenetic analysis based on inferences using LSU D2-D3 sequence data revealed that all 21 populations of Meloidogyne in this study are in a monophyletic clade in relation to other RKNs.

References:
Adam M.A.M., Phillips M.S., Blok V.C. (2005). Identification of Meloidogyne spp. from North East Libya and comparison of their inter- and intra-specific genetic variation using RAPDs. Nematology, 7: 599–609.
 
Adam M. A. M., Phillips M. S., Blok V. C. (2007): Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathology, 56, -  https://doi.org/10.1111/j.1365-3059.2006.01455.x
 
Berry Shaun D., Fargette Mireille, Spaull Vaughan W., Morand Serge, Cadet Patrice (2008): Detection and quantification of root-knot nematode (Meloidogyne javanica), lesion nematode (Pratylenchus zeae) and dagger nematode (Xiphinema elongatum) parasites of sugarcane using real-time PCR. Molecular and Cellular Probes, 22, 168-176  https://doi.org/10.1016/j.mcp.2008.01.003
 
Chen Y., Yu S., Chen H., Zhang S. (1997): Studies on the comparative identification and distribution of root-knot (Meloidogyne spp.) on tobacco in Yunnan Province. Journal of Chinese Tobacco, 3: 33–40. (In Chinese)
 
Blok V.C., Powers T.O. (2009): Biochemical and molecular identification. In: Perry R.N., Moens M., Starr J.L. (eds): Root-knot Nematodes. Chapter 4. Wallingford, CAB International: 98–118.
 
Filho Jerônimo Vieira de Araújo, Machado Andressa Cristina Zamboni, Dallagnol Leandro José, Aranha Camargo Luís Eduardo (2016): Root-Knot Nematodes ( Meloidogyne spp.) Parasitizing Resistant Tobacco Cultivars in Southern Brazil. Plant Disease, 100, 1222-1231  https://doi.org/10.1094/PDIS-03-15-0341-RE
 
De Ley I.T., Karssen G., De Ley P., Vierstraete A., Waeyenberge L., Moens M., Vanfleteren J. (1999): Phylogenetic analyses of internal transcribed spacer region sequences within Meloidogyne. Journal of Nematology, 31: 530–531.
 
Díaz-Silveira M.F., Herrera J.O. (1998): An overview of nematological problems in Cuba. Nematologica, 28: 151–164.
 
Eisenback J.D. (1985): Diagnostic characters useful in the identification of the four most common species of root-knot nematodes (Meloidogyne spp.). In: Sasser J.N., Carter C.C. (eds): An Advanced Treatise on Meloidogyne, Vol. I: Biology and Control. Raleigh, North Carolina State University: 95–112.
 
Esbenshade P., Triantaphyllou A. (1985): Use of enzyme phenotypes for identification of Meloidogyne species. Journal of Nematology, 17: 6–20.
 
Esser R.P., Perry V.G., Taylor A.L. (1976): A diagnostic compendium of the genus Meloidogyne (Nematoda: Heteroderidae). Proceedings of Helminthological Society of Washington, 43: 138–150.
 
Hu X., Yu S., Bai W. (1998): Studies on resistance of flue-cured and sun-cured tobacco varieties to common root-knot nematodes. In: Symposium on 6th Congress and Academic Annual Meeting of Chinese Society of Plant Pathology. Mar. 1, 1998, Peking, China: 268–271. (in Chinese)
 
Hu M. X., Zhuo K., Liao J. L. (2011): Multiplex PCR for the Simultaneous Identification and Detection of Meloidogyne incognita, M. enterolobii , and M. javanica Using DNA Extracted Directly from Individual Galls. Phytopathology, 101, 1270-1277  https://doi.org/10.1094/PHYTO-04-11-0095
 
Huelsenbeck J. P., Ronquist F. (2001): MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754-755  https://doi.org/10.1093/bioinformatics/17.8.754
 
Hugall A., Stanton J., Moritz C. (1999): Reticulate evolution and the origins of ribosomal internal transcribed spacer diversity in apomictic Meloidogyne. Molecular Biology and Evolution, 16, 157-164  https://doi.org/10.1093/oxfordjournals.molbev.a026098
 
Hunt D.J., Handoo Z.A. (2009): Taxonomy, Identification and principal species. In: Perry R.N., Moens M., Starr J.L. (eds): Root-knot Nematodes. Chapter 3. Wallingford, CAB International: 55–97.
 
Janssen Toon, Karssen Gerrit, Verhaeven Myrtle, Coyne Danny, Bert Wim (2016): Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports, 6, -  https://doi.org/10.1038/srep22591
 
Kiewnick S., Holterman M., van den Elsen S., van Megen H., Frey J.E., Helder J. (2014): Comparison of two short DNA barcoding loci (COI and COII) and two longer ribosomal DNA genes (SSU & LSU rRNA) for specimen identification among quarantine root-knot nematodes (Meloidogyne spp.) and their close relatives. European Journal of Plant Pathology, 140: 97–110.
 
Larget B., Simon D. L. (1999): Markov Chasin Monte Carlo Algorithms for the Bayesian Analysis of Phylogenetic Trees. Molecular Biology and Evolution, 16, 750-759  https://doi.org/10.1093/oxfordjournals.molbev.a026160
 
Meng Q., Long H., Xu J. (2004): PCR assays for rapid and sensitive identification of three major root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria. Acta Phytopathologica Sinica, 34: 204–210. (in Chinese).
 
Moens M., Perry R.N., Starr J.L. (2009): Meloidogyne species – a diverse group of novel and important plant parasites. In: Perry R.N., Moens M., Starr J.L. (eds): Root-knot Nematodes. Chapter 1. Wallingford, CAB International: 1–17.
 
Posada David, Buckley Thomas R., Thorne Jeffrey (2004): Model Selection and Model Averaging in Phylogenetics: Advantages of Akaike Information Criterion and Bayesian Approaches Over Likelihood Ratio Tests. Systematic Biology, 53, 793-808  https://doi.org/10.1080/10635150490522304
 
Powers Tom (2004): NEMATODE MOLECULAR DIAGNOSTICS: From Bands to Barcodes. Annual Review of Phytopathology, 42, 367-383  https://doi.org/10.1146/annurev.phyto.42.040803.140348
 
Powers T., Harris T. (1993): A polymerase chain reaction method for the identification of five major Meloidogyne species. Journal of Nematology, 25: 1–6.
 
Powers T.O., Mullin P.G., Harris T. S., Sutton L.A., Higgins R.S. (2005): Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey. Journal of Nematology, 37: 226–235.
 
Qin X., Duan Y., Yang M. (1991): Primary identification of tobacco root-knot nematodes in Yunnan. Yunnan Agricultural Science and Technology, 1991 (4): 22–23. (in Chinese)
 
Shew H.D., Lucas G.B. (1991): Compendium of Tobacco Diseases. St. Paul, American Phytopathological Society.
 
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (2011): MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739  https://doi.org/10.1093/molbev/msr121
 
Wang N., Shi J., Kong F., Wang C., Guo Y. (1998): Identification and distribution of tobacco root-knot nematodes in China. In: Prospect of Plant Protection in the 21st Century, and the 3rd National Symposium on Plant Protection Science and Technology for Young Plant Protection Workers. Oct. 8, 1998, Peking, China. (in Chinese)
 
Williams B.D., Schrank B., Huynh C., Shownkeen R., Waterston R.H. (1992): A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics, 131: 609–624.
 
Ye Weimin, Giblin-Davis Robin M., Braasch Helen, Morris Krystalynne, Thomas W. Kelley (2007): Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution, 43, 1185-1197  https://doi.org/10.1016/j.ympev.2007.02.006
 
Ye Weimin, Zeng Yongsan, Kerns James, Castagnone-Sereno Philippe (2015): Molecular Characterisation and Diagnosis of Root-Knot Nematodes (Meloidogyne spp.) from Turfgrasses in North Carolina, USA. PLOS ONE, 10, e0143556-  https://doi.org/10.1371/journal.pone.0143556
 
Zeng Yongsan, Ye Weimin, Kerns James, Tredway Lane, Martin Samuel, Martin Matt (2015): Molecular Characterization and Phylogenetic Relationships of Plant-Parasitic Nematodes Associated with Turfgrasses in North Carolina and South Carolina, United States. Plant Disease, 99, 982-993  https://doi.org/10.1094/PDIS-10-14-1060-RE
 
Zijlstra C., Uenk B.J., van Silfhout C.H. (1997): A reliable, precise method to differentiate species of root-knot nematodes in mixtures on the basis of ITS-RFLPs. Fundamental and Applied Nematology, 20: 59–63.
 
Fargette Mireille, Zijlstra Carolien, Donkers-Venne Dorine T.H.M. (2000): Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterised amplified region (SCAR) based PCR assays. Nematology, 2, 847-853  https://doi.org/10.1163/156854100750112798
 
download PDF

© 2020 Czech Academy of Agricultural Sciences