Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress

https://doi.org/10.17221/86/2014-PPSCitation:Koç E. (2015): Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress. Plant Protect. Sci., 51: 127-135.
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The effect of exogenous spermidine – Spd (0.1 and 1 mM) on the relation between polyaminoxidase (PAO), diaminoxidase (DAO), H2O2, and malondialdehyde (MDA) in three cultivars of pepper (Capsicum annum L.) exhibiting different tolerance to P. capsici stress: KM-Hot (P. capsici-tolerant), PM-217 (P. capsici-resistant), and CM-334 (P. capsici-highly resistant) was investigated. The 0.1 mM Spd pre-treatment led to an increase in DAO activity on the third day in three pepper cultivars under the stress of P. capsici, 1 mM Spd + P. capsici led to an increase in DAO and PAO activities on the fifth day if compared to P. capsici treatment alone. P. capsici alone caused an increase in the amounts of H2O2 at all times in all cultivars and in the amounts of MDA on the third and fifth days in all cultivars. Conversely; under the stress of P. capsici, pre-application of 0.1 mM Spd at all times in KM-Hot and CM-334 cultivars decreased the amount of MDA and H2O2 and on the first and third days in PM-217 cultivar decreased the amount of MDA and H2O2. This data indicates that exogenous Spd application before inoculation decreases the plasma membrane injury by decreasing the level of H2O2 and regulating the activities of amine oxidasein both P. capsici-sensitiveand P. capsici-resistant cultivars of peppers, so it may increase the tolerance of pepper cultivars against P. capsici.
Alcázar Rubén, Cuevas Juan C., Planas Joan, Zarza Xavier, Bortolotti Cristina, Carrasco Pedro, Salinas Julio, Tiburcio Antonio F., Altabella Teresa (2011): Integration of polyamines in the cold acclimation response. Plant Science, 180, 31-38  https://doi.org/10.1016/j.plantsci.2010.07.022
Angelini Riccardo, Cona Alessandra, Federico Rodolfo, Fincato Paola, Tavladoraki Paraskevi, Tisi Alessandra (2010): Plant amine oxidases “on the move”: An update. Plant Physiology and Biochemistry, 48, 560-564  https://doi.org/10.1016/j.plaphy.2010.02.001
Bestwick C. S. (): Localization of Hydrogen Peroxide Accumulation during the Hypersensitive Reaction of Lettuce Cells to Pseudomonas syringae pv phaseolicola. THE PLANT CELL ONLINE, 9, 209-221  https://doi.org/10.1105/tpc.9.2.209
Cona A. (): Polyamine Oxidase, a Hydrogen Peroxide-Producing Enzyme, Is Up-Regulated by Light and Down-Regulated by Auxin in the Outer Tissues of the Maize Mesocotyl. PLANT PHYSIOLOGY, 131, 803-813  https://doi.org/10.1104/pp.011379
Cona Alessandra, Rea Giuseppina, Angelini Riccardo, Federico Rodolfo, Tavladoraki Paraskevi (2006): Functions of amine oxidases in plant development and defence. Trends in Plant Science, 11, 80-88  https://doi.org/10.1016/j.tplants.2005.12.009
Cowley T., Walters D. R. (2002): Polyamine metabolism in barley reacting hypersensitively to the powdery mildew fungus Blumeria graminis f. sp. hordei. Plant, Cell and Environment, 25, 461-468  https://doi.org/10.1046/j.0016-8025.2001.00819.x
Gupta Kamala, Dey Abhijit, Gupta Bhaskar (2013): Plant polyamines in abiotic stress responses. Acta Physiologiae Plantarum, 35, 2015-2036  https://doi.org/10.1007/s11738-013-1239-4
Harrıgan W.F., McCane M.E. (1966): Laboratory Methods in Microbiology, Recipes to Stains, Reagents and Media. London and New York, Academic Press.
Heath R.L., Packer L. (1968): Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 180–98.
Holmstedt B., Larsson L., Tham R. (1961): Further studies of a spectrophotometric method for the determination of diamine oxidase activity. Biochimica et Biophysica Acta, 48, 182-186  https://doi.org/10.1016/0006-3002(61)90530-3
Hu Xiaohui, Zhang Yi, Shi Yu, Zhang Zhi, Zou Zhirong, Zhang Hao, Zhao Jiuzhou (2012): Effect of exogenous spermidine on polyamine content and metabolism in tomato exposed to salinity–alkalinity mixed stress. Plant Physiology and Biochemistry, 57, 200-209  https://doi.org/10.1016/j.plaphy.2012.05.015
Hussain Syed Sarfraz, Ali Muhammad, Ahmad Maqbool, Siddique Kadambot H.M. (2011): Polyamines: Natural and engineered abiotic and biotic stress tolerance in plants. Biotechnology Advances, 29, 300-311  https://doi.org/10.1016/j.biotechadv.2011.01.003
Jones D. R. (1975): Capsidiol Induction in Pepper Fruit during Interactions with Phytophthora capsici and Monilinia fructicola. Phytopathology, 65, 1417-  https://doi.org/10.1094/Phyto-65-1417
Kim Young Jin (1989): Expression of Age-Related Resistance in Pepper Plants Infected with Phytophthora capsici. Plant Disease, 73, 745-  https://doi.org/10.1094/PD-73-0745
Koç Esra, Üstün Ayşen Sülün, İşlek Cemil, Arıcı Yeliz Kaşko (2011): Defence responses in leaves of resistant and susceptible pepper (Capsicum annuum L.) cultivars infected with different inoculum concentrations of Phytophthora capsici Leon. Scientia Horticulturae, 128, 434-442  https://doi.org/10.1016/j.scienta.2011.02.008
Kongkıattıkajorn J. (2009): Effect of salinity stress on degradation of polyamines and amine oxidase activity in maize seedlings. Kasetsart Journal (Natural Science), 43: 28–33.
Kubi Jan (2005): The effect of exogenous spermidine on superoxide dismutase activity, H2O2 and superoxide radical level in barley leaves under water deficit conditions. Acta Physiologiae Plantarum, 27, 289-295  https://doi.org/10.1007/s11738-005-0005-7
Moschou P. N., Sarris P. F., Skandalis N., Andriopoulou A. H., Paschalidis K. A., Panopoulos N. J., Roubelakis-Angelakis K. A. (2009): Engineered Polyamine Catabolism Preinduces Tolerance of Tobacco to Bacteria and Oomycetes. PLANT PHYSIOLOGY, 149, 1970-1981  https://doi.org/10.1104/pp.108.134932
Palloix A., Daubeze A. M., Pochard E. (1988): Phytophthora Root Rot of Pepper Influence of Host Genotype and Pathogen Strain on the Inoculum Density-Disease Severity Relationships. Journal of Phytopathology, 123, 25-33  https://doi.org/10.1111/j.1439-0434.1988.tb01033.x
Paschalidis K. A. (2005): Sites and Regulation of Polyamine Catabolism in the Tobacco Plant. Correlations with Cell Division/Expansion, Cell Cycle Progression, and Vascular Development. PLANT PHYSIOLOGY, 138, 2174-2184  https://doi.org/10.1104/pp.105.063941
Rea G. (2004): Ectopic Expression of Maize Polyamine Oxidase and Pea Copper Amine Oxidase in the Cell Wall of Tobacco Plants. PLANT PHYSIOLOGY, 134, 1414-1426  https://doi.org/10.1104/pp.103.036764
Rodríguez-Kessler Margarita, Ruiz Oscar A., Maiale Santiago, Ruiz-Herrera José, Jiménez-Bremont Juan Francisco (2008): Polyamine metabolism in maize tumors induced by Ustilago maydis. Plant Physiology and Biochemistry, 46, 805-814  https://doi.org/10.1016/j.plaphy.2008.05.012
Roychoudhury Aryadeep, Basu Supratim, Sengupta Dibyendu N. (2011): Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. Journal of Plant Physiology, 168, 317-328  https://doi.org/10.1016/j.jplph.2010.07.009
Sujkowska-Rybkowska Marzena, Borucki Wojciech (2014): Localization of hydrogen peroxide accumulation and diamine oxidase activity in pea root nodules under aluminum stress. Micron, 57, 13-22  https://doi.org/10.1016/j.micron.2013.09.007
Velikova V, Yordanov I, Edreva A (2000): Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Science, 151, 59-66  https://doi.org/10.1016/S0168-9452(99)00197-1
Walters Dale (2003): Resistance to plant pathogens: possible roles for free polyamines and polyamine catabolism. New Phytologist, 159, 109-115  https://doi.org/10.1046/j.1469-8137.2003.00802.x
Wang Xue, Shi Guoxin, Xu Qinsong, Hu Jinzhao (2007): Exogenous polyamines enhance copper tolerance of Nymphoides peltatum. Journal of Plant Physiology, 164, 1062-1070  https://doi.org/10.1016/j.jplph.2006.06.003
Ward E.W.B., Stoessl A. (1974): Isolation of the phyoalexin capsidiol from pepper leaves and stems. In: 66th Annual Meeting American Phytopathological Society, Aug 11–15, 1974, Vancouver, Canada: 11–15.
Xu Xiaoying, Shi Guoxin, Ding Chunxia, Xu Ye, Zhao Juan, Yang Haiyan, Pan Qiuhong (2011): Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress. Plant Growth Regulation, 63, 251-258  https://doi.org/10.1007/s10725-010-9522-5
Yoda H. (2003): Induction of Hypersensitive Cell Death by Hydrogen Peroxide Produced through Polyamine Degradation in Tobacco Plants. PLANT PHYSIOLOGY, 132, 1973-1981  https://doi.org/10.1104/pp.103.024737
Yoda H. (2006): Polyamine Oxidase Is One of the Key Elements for Oxidative Burst to Induce Programmed Cell Death in Tobacco Cultured Cells. PLANT PHYSIOLOGY, 142, 193-206  https://doi.org/10.1104/pp.106.080515
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