Insight into epidemiological importance of phytoplasma vectors in vineyards in South Moravia, Czech RepublicŠafářová D., Lauterer P., Starý M., Válová P., Navrátil M. (2018): Insight into epidemiological importance of phytoplasma vectors in vineyards in South Moravia, Czech Republic. Plant Protect. Sci., 54: 234-239.
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Bois noir (BN), caused by ‘Candidatus Phytoplasma solani’, is a serious disease of grapevines in Europe. During the 2010–2012 survey in Perná vineyard (South Moravia, Czech Republic) a total of 4854 insect individuals were collected and among these, 95 insect species belonging to Auchenorrhyncha (77 species), Heteroptera (12), and Psylloidea (62) were indentified. The nested polymerase chain reaction-restriction fragment length polymorphism analyses confirmed Hyalesthes obsoletus as the main BN vector with 43.8% of phytoplasma positive individuals on average. A significant role of Anaceratagallia ribauti (22.6% of phytoplasma positive specimens) should be taken into account based on its occurrence and incidence of infected individuals. Eleven insect species were identified as new carriers of ‘Ca. P. solani’ or suggested as potential BN vectors in this work.

Aryan A., Brader G., Mörtel J., Pastar M., Riedle-Bauer M. (2014): An abundant ‘Candidatus Phytoplasma solani’ tuf b strain is associated with grapevine, stinging nettle and Hyalesthes obsoletus. European Journal of Plant Pathology, 140, 213-227
Atanasova Biljana, Jakovljević Miljana, Spasov Dušan, Jović Jelena, Mitrović Milana, Toševski Ivo, Cvrković Tatjana (2015): The molecular epidemiology of bois noir grapevine yellows caused by ‘Candidatus Phytoplasma solani’ in the Republic of Macedonia. European Journal of Plant Pathology, 142, 759-770
Batlle A., Martinez M.A., Lavina A. (2000): Occurrence, distribution and epidemiology of grapevine yellows in Spain. European Journal of Plant Pathology, 106: 811–816.
Batlle A., Altabella N., Sabaté J., Laviña A. (2008): Study of the transmission of stolbur phytoplasma to different crop species, by Macrosteles quadripunctulatus. Annals of Applied Biology, 152, 235-242
Chuche J., Danet J.-L., Salar P., Foissac X., Thiéry D. (2016): Transmission of ‘ Candidatus Phytoplasma solani’ by Reptalus quinquecostatus ( Hemiptera : Cixiidae ). Annals of Applied Biology, 169, 214-223
Cvrković A., Jović J., Mitrović M., Krstić O., Krnjalić S., Toševski I. (2011): Potential new hemipteran vectors of stolbur phytoplasma in Serbian vineyards. Bulletin of Insectology, 64 (Supplement): S129–S130.
Duduk B., Bulajić A., Duduk N., Calari A., Paltrinieri S., Krstić B., Bertaccini A. (2007): Identification of phytoplasmas belonging to aster yellows ribosomal group in vegetables in Serbia. Bulletin of Insectology, 60: 341–342.
Fialová R., Válová P., Balakishiyeva G., Danet J.-L., Šafárová D., Foissac X., Navrátil M. (2009): Genetic variability of stolbur phytoplasma in annual crop and wild plant species in south Moravia. Journal of Plant Pathology, 91: 411–416.
Fos A. (1992): Use of a Monoclonal Antibody to Detect the Stolbur Mycoplasmalike Organism in Plants and Insects and to Identify a Vector in France. Plant Disease, 76, 1092-
Galetto L., Marzachì C., Demichelis S., Bosco D. (2011): Host Plant Determines the Phytoplasma Transmission Competence of Empoasca decipiens (Hemiptera: Cicadellidae). Journal of Economic Entomology, 104, 360-366
Ge Q., Maixner M. (2003): Comparative experimental transmission of grapevine yellows phytoplasmas to plants and artificial feeding medium. In: Extended Abstracts – 14th Meeting of the International Council for the Study of Virus and Virus-Like Diseases of the Grapevine (ICVG), Sept 12–17, 2003, Locorotondo, Italy: 109–110. Available at (accessed Dec 5, 2017).
Kostadinovska E., Quaglino F., Mitrev S., Casati P., Bulgari D., Bianco P.A. (2014): Multiple gene analyses identify distinct Bois noir phytoplasma genotypes in the Republic of Macedonia. Phytopathologia Mediterranea, 53: 300–310.
Kuntzmann K., Bogen E., Renel C. (2008): Bois noir disease of the grapevine in Alsace: field transmission, observations made on symptomatology and reduction of transmission risk by the vector Hyalesthes obsoletus Signoret. IOBC/WPRS Bulletin, 36: 127–136.
Landi L., Isidoro N., Riolo P. (2013): Natural Phytoplasma Infection of Four Phloem-Feeding Auchenorrhyncha Across Vineyard Agroecosystems in Central-Eastern Italy. Journal of Economic Entomology, 106, 604-613
Langer M., Maixner M. (2004): Molecular characterisation of grapevine yellows associated phytoplasmas of the stolbur group based on RFLP analysis of non-ribosomal DNA. Vitis, 43: 191–199.
Maixner M. (1994): Transmission of German grapevine yellows (Vergilbungskrankheit) by the planthopper Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae). Vitis, 33: 103–104.
Mehle Nataša, Ravnikar Maja, Seljak Gabrijel, Knapic Vlasta, Dermastia Marina (2011): The most widespread phytoplasmas, vectors and measures for disease control in Slovenia. Phytopathogenic Mollicutes, 1, 65-
Mitrović Milana, Jović Jelena, Cvrković Tatjana, Krstić Oliver, Trkulja Nenad, Toševski Ivo (2012): Characterisation of a 16SrII phytoplasma strain associated with bushy stunt of hawkweed oxtongue (Picris hieracioides) in south-eastern Serbia and the role of the leafhopper Neoaliturus fenestratus (Deltocephalinae) as a natural vector. European Journal of Plant Pathology, 134, 647-660
Mori N., Quaglino F., Tessari F., Pozzebon A., Bulgari D., Casati P., Bianco P.A. (2015): Investigation on ‘bois noir’ epidemiology in north-eastern Italian vineyards through a multidisciplinary approach. Annals of Applied Biology, 166, 75-89
ORENSTEIN S, ZAHAVI T, NESTEL D, SHARON R, BARKALIFA M, WEINTRAUB P G (2003): Spatial dispersion patterns of potential leafhopper and planthopper (Homoptera) vectors of phytoplasma in wine vineyards. Annals of Applied Biology, 142, 341-348
Orságová H., Březíková M., Schlesingerová G. (2011): Presence of phytoplasmas in hemipterans in Czech vineyards. Bulletin of Insectology, 64 (Supplement): S119–S120.
Riedle-Bauer M., Tiefenbrunner W., Otreba J., Hanak K., Schildberger B., Regner F. (2006): Epidemiological observations on Bois Noir in Austrian vineyards. Mitteilungen Klosterneuburg, 56: 177–181.
Riedle-Bauer Monika, Sára Anita, Regner Ferdinand (2008): Transmission of a Stolbur Phytoplasma by the Agalliinae Leafhopper Anaceratagallia ribauti (Hemiptera, Auchenorrhyncha, Cicadellidae). Journal of Phytopathology, 156, 687-690
Riolo P., Landi L., Nardi S., Isidoro N. (2007): Relationships between Hyalesthes obsoletus, its herbaceous host plants and “bois noir” phytoplasma strains in vineyard ecosystems in the Marche region (central-eastern Italy). Bulletin of Insectology, 60: 353–354.
Sabaté J., Laviña A., Legorburu J., Fortanete J., Perez de Obanos J.J., Perez Marín J.L., Reyes J., Batlle A. (2007): Incidence of “bois noir” phytoplasma in different wine-growing regions of Spain and its relation to Hyalesthes obsoletus. Bulletin of Insectology, 60: 367–368.
Safarova D., Lauterer P., Korbasova Z., Stary M., Valova P., Navratil M. (2011): Hyalesthes obsoletus, a vector of stolbur phytoplasma: current situation in South Moravia, Czech Republic. Bulletin of Insectology, 64: S127–S128.
Sforza R., Clair D., Daire X., Larrue J., Boudon-Padieu E. (1998): The Role of Hyalesthes obsoletus (Hemiptera: Cixiidae) in the Occurrence of Bois noir of Grapevines in France. Journal of Phytopathology, 146, 549-556
Starý M., Válová P., Šafářová D., Lauterer P., Ackermann P., Navrátil M. (2013): Survey and molecular detection of Bois noir in vineyards of the Czech Republic– Short communication. Horticultural Science, 40, 83-87
Ján Tancik, Gabriel Seljak (2017): Occurrence of Scaphoideus titanus Ball and some other Auchenorrhyncha in the vineyards of western Slovakia. Plant Protection Science, 53, 96-100
Tishechkin D.Y. (1998): Acoustic signals and morphological characters of leafhoppers belonging to Aphrodes bicinctus group (Homoptera, Cicadellidae) from European Russia. Entomological Review, 78: 370–377.
Valenta V., Musil M., Mišiga S. (1961): Investigations on European Yellows-type Viruses I. The Stolbur Virus. Journal of Phytopathology, 42, 1-38
Weintraub P.G. (2007): Insect vectors of phytoplasmas and their control – an update. Bulletin of Insectology, 60: 169–173.
Weintraub Phyllis G., Beanland LeAnn (2006): INSECT VECTORS OF PHYTOPLASMAS. Annual Review of Entomology, 51, 91-111
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