Genetic diversity analysis in blackgram (Vigna mungo L. Hepper) genotypes using microsatellite markers for resistance to Yellow Mosaic Virus

One hundred and two black gram genotypes were evaluated for Yellow Mosaic Virus (YMV) resistance based on disease scoring, molecular characterization, yield and morphological observation. Nine biometrical traits were studied for the analysis of variance and correlation. The genotypes showed highest genetic variation for single plant yield (Genotypic Coefficient Variation 42.72%). The genotypes were grouped into 18 clusters based on the morphological data. Genetic divergence among black gram genotypes was differentiated by 60 alleles using 14 polymorphic simple sequence repeat markers. The Polymorphism Information Content value varied from 0.37 to 0.79. Multivariate analyses of Simple Sequence Repeat (SSR) marker scorings divided the germplasm into five divergent clusters wherein, clusters viz., II, III and V accommodated 61 genotypes and all these genotypes were resistant to moderately resistant for YMV.  Tolerant accessions identified from this present investigation can directly be used for further crop improvement programmes

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