Gene flow was not detected from a field trial of transgenic plum cv. HoneySweet – Short Communicationínek P., Polák J., Komínková M., Scorza R. (2019): Gene flow was not detected from a field trial of transgenic plum cv. HoneySweet – Short Communication. Plant Protect. Sci., 55: 90-92.
supplementary materialdownload PDF

A field trial with plum cv. HoneySweet was established in 2003 in an experimental plot near Crop Research Institute in Prague-Ruzyně, Czech Republic, on the basis of a permission issued by the Ministry of the Environment of the Czech Republic. In addition to the evaluation of resistance of plum cv. HoneySweet to Plum pox virus, the field trial was used to evaluate gene flow of the inserted transgene. Sampling of blackthorn and myrobalan trees outside the field test site occurred at distances ranging from 544 m to 845 m from the test site and showed no gene flow, testing both plants and seeds collected from blackthorns and myrobalans. Similarly, seeds from plums cv. Jojo growing directly at the field test place did not show any presence of the transgene after seven years of evaluation. 

Chittka Lars, Gumbert Andreas, Kunze Jan (1997): Foraging dynamics of bumble bees: correlates of movements within and between plant species. Behavioral Ecology, 8, 239-249
HILL PEGGY S.M, WELLS PATRICK H, WELLS HARRINGTON (1997): Spontaneous flower constancy and learning in honey bees as a function of colour. Animal Behaviour, 54, 615-627
Jefferson Richard A. (1987): Assaying chimeric genes in plants: The GUS gene fusion system. Plant Molecular Biology Reporter, 5, 387-405
Minev I. (2007): Biological characteristics of perspective interspecific hybrids from genus Prunus. Journal of Pomology, 41: 157–158.
Nielson J., Olrik D.C. (2001): A morphometric analysis of Prunus spinosa, P. domestica ssp. insititia and their putative hybrids in Denmark. Nordic Journal of. Botany, 21: 349–363.
OECD (Organisation for Economic Co-operation and Development) (2002): Series on Harmonization of Regulatory Oversight in Biotechnology, No. 24. Consensus document on the biology of Prunus sp. (stone fruits). Paris.
Polák Jaroslav, Kundu Jiban K, Krška Boris, Beoni Eva, Komínek Petr, Pívalova Jitka, Jarošová Jana (2017): Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease. Journal of Integrative Agriculture, 16, 516-522
Scorza Ralph, Ravelonandro Michel, Callahan AnnM., Cordts JohnM., Fuchs Marc, Dunez Jean, Gonsalves Dennis (1994): Transgenic plums (Prunus domestica L.) express the plum pox virus coat protein gene. Plant Cell Reports, 14, -
Scorza R., Georgi L., Callahan A., Petri C., Hily J.M., Dardick C., Damsteegt V., Ravelonandro M. (2010): Hairpin Plum pox virus coat protein (hpPPV-CP) structure in 'HoneySweet' C5 plum provides PPV resistance when genetically engineered into plum (Prunus domestica) seedlings. Julius-Kühn Archiv, 427: 141–146.
Scorza R., Callahan A., Dardick C., Ravelonandro M., Polak J., Malinowski T., Zagrai I., Cambra M., Kamenova I. (2013a): Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum – from concept to product. Plant Cell, Tissue and Organ Culture, 115: 1–12.
Scorza Ralph, Kriss Alissa B., Callahan Ann M., Webb Kevin, Demuth Mark, Gottwald Tim, Vinatzer Boris Alexander (2013): Spatial and Temporal Assessment of Pollen- and Seed-Mediated Gene Flow from Genetically Engineered Plum Prunus domestica. PLoS ONE, 8, e75291-
Scorza Ralph, Ravelonandro Michel, Callahan Ann, Zagrai Ioan, Polak Jaroslav, Malinowski Tadeuz, Cambra Mariano, Levy Laurene, Damsteegt Vern, Krška Boris, Cordts John, Gonsalves Dennis, Dardick Chris (2016): ‘HoneySweet’ (C5), the First Genetically Engineered Plum pox virus–resistant Plum (Prunus domestica L.) Cultivar. HortScience, 51, 601-603
Stout Jane C., Allen John A., Goulson Dave (1998): The influence of relative plant density and floral morphological complexity on the behaviour of bumblebees. Oecologia, 117, 543-550
supplementary materialdownload PDF

© 2020 Czech Academy of Agricultural Sciences