Selection of AFLP markers linked with crown rust resistance in Lolium: efficiency of bulk segregant analysis in an Allogamous species

https://doi.org/10.17221/10345-PPSCitation:Muylle H., van Bockstaele E., Roldan-Ruiz I. (2002): Selection of AFLP markers linked with crown rust resistance in Lolium: efficiency of bulk segregant analysis in an Allogamous species. Plant Protect. Sci., 38: 161-164.
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Ryegrasses are important forage species. An important foliar disease in Lolium is crown rust, caused by the fungus Puccinia
coronata. This cosmopolitan biotrophic fungus causes forage and seed losses, and decoloration of amenity grasslands.
In breeding towards resistant cultivars, it is important to know the mode of inheritance of crown rust resistance. A pair
cross between a susceptible and a resistant plant resulted in a F1 population segregating for rust resistance. According to
the segregation data, major genes were assumed to encode for crown rust resistance. In a bulk segregant analysis (BSA)
187 primer combinations revealed AFLP markers linked with resistance. Two groups of genetically unlinked markers
were identified, explaining together 35% of the phenotypic variation.
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