Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosum

https://doi.org/10.17221/10351-PPSCitation:Germundsson A., Sandgren M., Barker H., Savenkov E.I., Valkonen J.P.T. (2002): Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosum. Plant Protect. Sci., 38: 183-185.
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Resistance to Potato mop-top virus (PMTV) was studied in Nicotiana benthamiana and potato (Solanum tuberosum cv.
Saturna) transformed with the coat protein (CP) gene of PMTV. In N. benthamiana plants mechanically inoculated with
PMTV, RNA2 was detected in leaves and roots in several plants in the absence of RNA3. When N. benthamiana was
grown in infested soil, viral RNA was detected in roots, but no systemic movement of PMTV to above-ground parts was
observed. The incidence of PMTV infections was reduced in the CP-transgenic potato plants grown in an infested field
in Sweden. However, in infected tubers, all three virus RNAs were detected.
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