A study of resistant response of Raphanus sativus cv. Siletina hairy roots obtained after transformation with Agrobacterium rhizogenes to infection with Heterodera schachtii (abstract only)

Citation:Lichocka M., Grundler F., Golinowski W. (2002): A study of resistant response of Raphanus sativus cv. Siletina hairy roots obtained after transformation with Agrobacterium rhizogenes to infection with Heterodera schachtii (abstract only). Plant Protect. Sci., 38: 241-241.
Nematode susceptible oilseed radish (R. sativus) cv. Siletina was transformed with A. rhizogenes containing barnase-barstar gene construct. This construct, due to specific gene regulation, is supposed to cause the breakdown of the initial syncytial cell induced by H. schachtii. Hairy roots containing barnase-barstar gene construct and roots obtained after transformation with wild-type bacteria were inoculated with H. schachtii juveniles. Only a limited number of adult females developed that indicates resistant response of transformed roots. Similarly as observed in roots of resistant cv. Pegletta. This effect was independent from the nature of the transferred gene and was apparently caused by the transformation itself. Plant growth factors: kinetin, IAA, 2.4-D and methyl jasmonate externally applied before inoculation with H. schachtii did not influence the nematode sex ratio. Glucosinolate/myrosinase system is considered as a possible mechanism of plant resistance. However, HPLC analysis of glucosinolates composition of transformed and non-transformed roots indicated that the former had less complex glucosinolates pattern than the latter. It seems that this system is not a basic mechanism of observed resistant reaction.
The work is a part of the EU-project QLRT-1999-1501 (NONEMA).

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