Expression pattern of Le-Exp5 gene in syncytia induced by Globodera rostochiensisin in roots of susceptible tomato (abstract only)

Citation:Sobczak M., Fudali S., Golecki B., Janakowski S., Grymaszewska G., Kurek W., Lichocka M., Grundler F., Golinowski W. (2002): Expression pattern of Le-Exp5 gene in syncytia induced by Globodera rostochiensisin in roots of susceptible tomato (abstract only). Plant Protect. Sci., 38: 242-242.
The expression pattern of the tomato expansin gene Le-Exp5 was examined on resin and agar embedded sections of syncytia induced by Ro1 pathotype of G. rostochiensis in roots of susceptible tomato cvar. Moneymaker. In 1 and 3 day-old syncytia strong expression of Le-Exp5 took place in cells incorporated into syncytium and in some neighbouring cells. In 7 day-old syncytia the older parts of syncytia contained relatively less Le-Exp5 mRNA than cells situated at distal parts of syncytia that had been recently incorporated into it. Strong expression of Le-Exp5 took place also in proliferating and dividing parenchymatous cells surrounding syncytia. These results were gained in experiments where in situ RT-PCR was followed by in situ hybridisation, which confirmed the RT-PCR data.
The work is a part of the EU-project QLRT-1999-1501 (NONEMA).

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti