Detection of AFLP markers linked to Plum pox virus resistance genes in apricot (abstract only)
J. Salava, E. Wang, B. Krška, J. Polák, J. Komínek, A.G. AbbottCitation:Salava J., Wang E., Krška B., Polák J., Komínek J., Abbott A.G. (2002): Detection of AFLP markers linked to Plum pox virus resistance genes in apricot (abstract only). Plant Protect. Sci., 38: 246-246.
Plum pox virus (PPV) is one of the most important problems to fruit production worldwide. We combined “amplified fragment length polymorphism (AFLP) technology” and “bulked segregant analysis” in order to detect AFLP bands as markers linked to PPV resistance genes in apricot (Prunus armeniaca L.). A backcross between an apricot cultivar Stark Early Orange and a selection LE-3218 was made at Faculty of Horticulture Mendel University of Agriculture and Forestry in Lednice na Moravě in 1998. “Stark Early Orange” is resistant to PPV, LE-3218 is susceptible to PPV. The apricot seedlings obtained from the cross were grown in a greenhouse. They were inoculated with PPV by aphids and by chip budding. PPV infection was evaluated through ELISA, and by observation of symptom expression in three consecutive growth periods. Bulked segregant analysis using 271 primer combinations revealed 11 markers to be polymorphic between both the bulks as well as the parents. Two (EAT/MCCT, ETC/MCCT) of the polymorphic markers were highly reproducible and were detected in all resistant BC1 individuals. These markers will be mapped on different apricot molecular marker maps to determine the number of marked resistance loci. Using BAC libraries of apricot and peach, these markers will be converted to microsatellite markers to facilitate marker-assisted selection in breeding programs.