Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta
A.M. Hietala, M. Eikenes, M. Kvaalen, H. Solheim, C.G. Fossdalhttps://doi.org/10.17221/10507-PPSCitation:Hietala A.M., Eikenes M., Kvaalen M., Solheim H., Fossdal C.G. (2002): Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta. Plant Protect. Sci., 38: 406-407.
A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and 1 ng of host DNA in multiplex conditions. As a comparative method for quantifying fungal colonization, we applied the ergosterol assay. There was a very high correlation between the results obtained with the two methods, this strengthening the credibility of both assays. The advantages and disadvantages of these assays are discussed.Keywords:
Heterobasidion; Norway spruce; infection; quantification