Characterization of two closely linked soybean pgip genes and transcript regulation following pathogen infection and wounding
D. Ovidio R, S. Roberti, M. Melaragni, C. Capodicasa, L.S. Sella, F. Favaronhttps://doi.org/10.17221/10529-PPSCitation:Ovidio R D., Roberti S., Melaragni M., Capodicasa C., Sella L.S., Favaron F. (2002): Characterization of two closely linked soybean pgip genes and transcript regulation following pathogen infection and wounding. Plant Protect. Sci., 38: 480-482.
Polygalacturonase-inhibiting protein (PGIP) is a plant cell wall protein that regulates the action of fungal endopolygalacturonases (PG) favouring the formation of oligogalacturonides active as elicitors of plant defense responses. We have isolated two novel soybean PGIP genes (Gmpgip1 and Gmpgip2) and their recognition specificities against fungal PGs have been tested by expressing them in Nicotiana benthamiana using the Potato virus X (PVX) as vector and in Escherichia coli. In both systems GmPGIP1 and GmPGIP2 accumulate but showed no activity against PGs. Transcript regulation of GmPGIP1 and GmPGIP2 showed that these genes undergo a differential regulation following Sclerotinia sclerotiorum infection and wounding.Keywords:
Sclerotinia sclerotiorum; Glycine max; PGIP; polygalacturonase; gene family; transcript regulation