Isolation of differentially expressed genes involved in clubroot disease
A. Schuller, J. Ludwig-Müllerhttps://doi.org/10.17221/10530-PPSCitation:Schuller A., Ludwig-Müller J. (2002): Isolation of differentially expressed genes involved in clubroot disease. Plant Protect. Sci., 38: 483-486.
The interaction between Plasmodiophora brassicae and its host Brassica rapa is investigated by two strategies. (1) IAA-conjugate hydrolases: Root hypertrophy in club root disease is dependent on increased auxin levels and these could result from auxin-conjugate hydrolysis. So far we isolated 5 different cDNA fragments out of various tissues which revealed high identity to IAR3/ILL5, ILL2, ILL3, ILL6 and ILR1 genes from Arabidopsis by comparison with database entries. (2) Random priming: Using this method, we have so far obtained 26 clones from clubroot tissue, from which several sequences may be components of plant signal transduction chains, metabolic pathways and transcriptional regulation.Keywords:
Plasmodiophora brassicae; Brassica rapa; IAA-amidohydrolases; random priming