Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein
M. Paape, S. Nell, S. von Bargen, J.-W. Kellmannhttps://doi.org/10.17221/10331-PPSCitation:Paape M., Nell S., von Bargen S., Kellmann J.-. (2002): Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein. Plant Protect. Sci., 38: S108-S111.
To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.Keywords:
Tomato spotted wilt virus (TSWV); Arabidopsis thaliana; Nicotiana rustica; movement protein; J-domain protein; yeast two-hybrid system