Improved diagnostic tools for the certification of strawberry propagation material – the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)

https://doi.org/10.17221/10313-PPSCitation:Vašková D., Špak J. (2002): Improved diagnostic tools for the certification of strawberry propagation material – the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV). Plant Protect. Sci., 38: S24-S27.
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Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBV-NASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR.
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