Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography
J. Lochman, O. Šerý, L. Jankovský, V. Mikeshttps://doi.org/10.17221/10316-PPSCitation:Lochman J., Šerý O., Jankovský L., Mikes V. (2002): Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography. Plant Protect. Sci., 38: S31-S34.
The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. We analysed about 40 isolates of Armillaria collected in Czech Republic by PCR and restriction analysis using gel electrophoresis and ion-exchange HPLC. Restrictase Hinf I was able to discriminate all investigated Armillaria species. The sensitivity and resolution of HPLC method was better than that performed by gel electrophoresis. HPLC was able to detect some heterozygous. The results prove the similarity of the species A. borealis, A. cepistipes, A. gallica, A. ostoyae in difference of A. mellea and A. tabescens.Keywords:
Armillaria; PCR; forest pathogen; basidiomycete; root rot disease