We analysed scientific papers published in the “Plant Protection Science” (PPS), former “Ochrana Rostlin” which is the only international scientific journal covering the problematic of the plant protection in the Czech Republic (CZ). The aim of this study was to explore general trends in the plant protection research in CZ during the five past decades (i.e. 1950–2002). During the period studied, 1633 articles and 2425 authors appeared in PPS. The peak of the annual publishing quantity was in 1970s and 1980s. The number of papers per year declined in 1990s reflecting (i) a decrease of scientific institutes and restriction of agricultural research in the CZ in early 1990s, and (ii) increasing demands on the quality of PPS in this period. The publication proportion of various disciplines in PPS were as follows: mycology (34.3%), entomology (20.9%), virology (20.9%), weed science (13.7%), bacteriology (4.9%), agroecology (3.2%), stored-product protection (1.7%), rodent control (0.2%), air-pollution derived injuries (0.1%). The relative contributions of the individual disciplines were fairly steady across the period studied except for the increased publishing share of the stored product protection. We found a decreasing trend in the publishing of pesticide papers, and an increasing trend to publish papers by more than one author. The global process of integration and internationalisation of applied sciences was reflected by PPS via (i) replacement of the national (OR) title with the English title (PPS) of the journal, (ii) increasing number of foreign authors, and (iii) increasing proportion of scientific papers in English, reaching 100% in 1999. Most of the changes leading to internationalisation of the journal PPS were traceable after 1989s with the termination of a “cold war” inEurope.