Development of PCR for specific determination of root-knot nematode Meloidogyne incognita
Blanka Tesařová, Miloslav Zouhar, Pavel Ryšánekhttps://doi.org/10.17221/3823-PPSCitation:Tesařová B., Zouhar M., Ryšánek P. (2003): Development of PCR for specific determination of root-knot nematode Meloidogyne incognita. Plant Protect. Sci., 39: 23-28.
PCR primers designed from the gene sequence for the SEC 1 oesophageal gland protein were used to specifically detect and differentiate the root-knot nematode Meloidogyne incognita from other species from the genus Meloidogyne. Amplification products were obtained from five M. incognita populations from different origins whereas DNA from M. fallax, M. javanica, M. arenaria, M. chitwoodi and M. hapla was not amplified. DNA extracted from different materials (females, root galls and spiked soil) could easily be used for M. incognita detection. One female gave sufficient amount of DNA for detection. Together with mitochondrial DNA this is one of the first attempts to use a gene outside of ITS regions for species specific PCR in the genus Meloidogyne.Keywords:
root-knot nematodes; Meloidogyne incognita; PCR; diagnostics; oesophageal gland protein gene