Phytoplasma occurrence in apple trees in the Czech Republic
Renata Fialová, Milan Navrátil, Pavla Válováhttps://doi.org/10.17221/3820-PPSCitation:Fialová R., Navrátil M., Válová P. (2003): Phytoplasma occurrence in apple trees in the Czech Republic. Plant Protect. Sci., 39: 7-12.
The presence of phytoplasmas in apple trees with proliferation symptoms, rubbery wood symptoms and no symptoms was determined by using polymerase chain reaction assays with primers amplifying phytoplasma 16S rRNA gene. Phytoplasmas were detected in all trees with proliferation symptoms. Positive tests for phytoplasma in the group of trees with rubbery wood symptoms and of those without symptoms revealed a relatively high incidence of latent phytoplasma infection. Using restriction fragment length polymorphism analysis, phytoplasma of the same identity – apple proliferation phytoplasma (subgroup 16SrX-A) – was recorded in all positively tested trees.Keywords:
proliferation symptoms; rubbery wood symptoms; non-symptomatic infection; PCR; RFLP